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Establishment of Evaluation of Endothelial Function in Clinical Laboratory Medicine.

临床检验医学内皮功能评价的建立。

基本信息

批准号：
13470521
负责人：
OSHIMA Tetsuya
金额：
$ 6.98万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470521/
关键词：
endothelium atherosclerosis hyperemia nitric oxide platelets hypertension ultrasound aging 高脂血症

项目摘要

1. The methods of evaluation of vascular endothelial function in humans are different among different laboratories. In order to establish the evaluation method of endothelial function, we have standardized the measurement of endothelium-dependent and -independent vasodilation, evaluated the reference value, and studied the effects of several risk factors for atherosclerosis.2. Using high resolution ultrasound, we determined the response of brachial artery to hyperemia (Flow mediated vasodilation : FMD) as endothelium-dependent vasodilation and the response to sublingual spray of nitroglycerine (NTG) as endothelium-independent vasocilation in healthy subjects and patients with risk factors for atherosclerosis. Reactive hyperemia was evoked by release of cuff inflated to 200 mmHg for 5 minutes on the upper arm.3. The peak response was achieved about 1 minute after hyperemia and about 2〜3 minutes after NTG. As in 90% of healthy subjects, both FMD and the response to NTG ranged between 10 and 15%, these values are considered as the reference range.4. In healthy subjects, there was no gender difference in FMD or the response to NTG. Age was significantly and negatively correlated with FMD and the response to NTG, but had no relation to the index of endothelial function (the ratio of FMD to NTG).5. In comparison with healthy subjects, FMD and EFI were decreased in patients with essential hypertension, diabetes and hyperlipidemia, but the response to NTG were similar in all groups.6. We have established the method to evaluate the endothelial function of brachial artery in humans as the routine clinical laboratory examination. We clarified that the vascular response is decreased with aging, and endothelial function was disturbed in risk factors for atherosclerosis.

1.不同的实验室评价人体血管内皮功能的方法不同。为建立内皮功能的评价方法，我们对内皮依赖性和非依赖性血管舒张功能的测定方法进行了标准化，评估了其参考值，并研究了几种危险因素对动脉粥样硬化的影响.使用高分辨率超声，我们确定了健康受试者和有动脉粥样硬化危险因素的患者肱动脉对充血的反应（血流介导的血管舒张：FMD）作为内皮依赖性血管舒张和对硝酸甘油（NTG）舌下喷雾的反应作为内皮非依赖性血管舒张。在上臂释放充气至200 mmHg的袖带5 min，诱发反应性充血.充血后约1分钟和NTG后约2 ~ 3分钟达到峰值反应。在90%的健康受试者中，FMD和对NTG的反应范围在10 - 15%之间，这些值被认为是参考范围。在健康受试者中，FMD或对NTG的反应没有性别差异。年龄与FMD和对NTG的反应呈显著负相关，但与内皮功能指标（FMD与NTG的比值）无关。5.与健康人相比，高血压、糖尿病和高脂血症患者的FMD和THR均降低，但对NTG的反应在各组中相似.我们建立了评价人肱动脉内皮功能的方法，作为临床常规实验室检查。我们阐明了血管反应随着年龄的增长而降低，内皮功能在动脉粥样硬化的危险因素中受到干扰。