Vascularization of Regenerated Tissue by Therapeutic Angiogenesis based on the Bio-Regulation System

基于生物调节系统的治疗性血管生成使再生组织血管化

• 批准号: 15390540
• 负责人: ICHIOKA Shigeru
• 金额: $ 4.48万
• 依托单位: Saitama Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390540/
• 关键词: Angiogenesis; Regenerative Medicine; Bone Marrow; 組織工学; 生体制御システム; 微小循環

This study attempted to clarify the effects of therapeutic neovascularization by bone marrow cells for salvaging flaps after ischemia-reperfusion injury. Bone marrow mononuclear cell layer (endothelial progenitor cell-enriched fraction) was isolated from the mouse femur and tibia. Symmetrical double flaps were elevated in mice. Each flap topically received phosphate buffered saline (PBS) or bone marrow cells in PBS. Flaps were subjected to 6-hour ischemia and subsequent reperfusion. On the 7^<th> postoperative day, the flap survival area was measured (n=27). The mean survival area of bone marrow cells -transplanted flaps was 66.3 ± 18.0 % whereas control flaps showed a survival area of 49.7± 22.2 %. The difference was highly significant (p=0.000209). Histological examination revealed the average vascular density of bone marrow cells-transplanted flaps had significantly increased. The present study proved bone marrow cells acted with significant efficacy in promoting the survival of ischemia-reperfusion-mediated damaged tissue

本研究试图阐明骨髓细胞治疗性新生血管化对缺血再灌注损伤后皮瓣修复的作用。从小鼠股骨和胫骨分离骨髓单核细胞层（内皮祖细胞富集部分）。对称双皮瓣在小鼠中升高。每个皮瓣局部接受磷酸盐缓冲盐水（PBS）或PBS中的骨髓细胞。皮瓣进行6小时的缺血和随后的再灌注。术后第7<th>天，测量皮瓣成活面积（n=27）。骨髓细胞移植皮瓣的平均存活面积为66.3 ± 18.0%，而对照皮瓣的存活面积为49.7± 22.2%。差异具有高度显著性（p=0.000209）。组织学检查显示移植骨髓细胞的皮瓣平均血管密度明显增加。本研究证明骨髓细胞对缺血再灌注损伤组织的存活具有明显的促进作用

项目成果

A symmetrical dorsal island double flap in the mouse.

小鼠的对称背岛双瓣。

• 发表时间: 2004
• 期刊: Eur J Plast Surg 27
• 作者: S.Ichioka;S.Kudo;K.Tokioka;M.Shibata;et al.
• 通讯作者: et al.

Ichioka S et al.: "Skin temperature changes induced by strong static magnetic field exposure."Bioelectromagnetics. 24. 380-386 (2003)

Ichioka S 等人：“强静磁场暴露引起的皮肤温度变化。”生物电磁学。

市岡 滋: "Prefabricated flapにおける血管新生のメカニズム"形成外科. 47. 131-137 (2004)

Shigeru Ichioka：“预制皮瓣的血管生成机制”整形外科。 47. 131-137 (2004)

市岡 滋: "褥瘡難治症例の処置"形成外科. 46. 485-490 (2003)

Shigeru Ichioka：“顽固性压疮病例的治疗”《整形外科》46. 485-490 (2003)。

The positive experience using a growth factor product on deep wounds with bone exposure.

使用生长因子产品治疗骨外露的深部伤口的积极体验。

• 发表时间: 2005
• 期刊: Journal of Wound Care 14
• 作者: Ichioka S;et al.
• 通讯作者: et al.