Study on Mechanisms of Angiogenesis Induced by Hemodynamic Stress and Hypoxia

血流动力学应激和缺氧诱导血管生成机制的研究

• 批准号: 13470379
• 负责人: ICHIOKA Shigeru
• 金额: $ 4.54万
• 依托单位: Saitama Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470379/
• 关键词: microcirculation; angiogenesis; hemodynamic stress; oxygen tension; hypoxia; 生体顕微鏡; 血行動態; 微小循環; shear stress; 組織酵素分圧

Although angiogenesis is one of the major concerns in various fields of medicine, its mechanism has not been completely clarified. A living body is regarded as an integrated system to maintain homeostasis. As a part of the system, design of the vascular structure provides the optimum oxygen supply to the tissue. Considering from such a viewpoint, angiogenesis belongs to adaptive responses of vascular remodeling to realize the optimum microvasculature for the most efficient oxygen supply. We suppose the response of the endothelial cells to hemodynamic stress and hypoxia controls the angiogenic process.To investigate the effects of hemodynamic stress and tissue oxygen concentration, we have developed a new experimental model that allows direct visualization of flap microcirculation in mice by intravital microscopic techniques. Our technique can be regarded as a unique method of inducing and chronically observing angiogenesis in vivo and provides significant means for further study.

尽管血管生成是医学各个领域的主要关注点之一，但其机制尚未完全阐明。生物体被认为是一个完整的系统来维持体内平衡。作为系统的一部分，血管结构的设计为组织提供最佳的氧气供应。从这个角度考虑，血管生成属于血管重塑的适应性反应，以实现最佳的微血管系统以获得最有效的氧气供应。我们假设内皮细胞对血流动力学应激和缺氧的反应控制了血管生成过程，为了研究血流动力学应激和组织氧浓度的影响，我们开发了一种新的实验模型，可以通过活体显微技术直接观察小鼠皮瓣的微循环。该技术可作为一种独特的体内诱导和长期观察血管生成的方法，为进一步研究提供了重要手段。

项目成果

Ichioka, S. et al.: "In vivo model for visualizing flap microcirculation of ischemia-reperfusion"Microsurgery. 22. 304-310 (2002)

Ichioka, S. 等人：“用于可视化缺血再灌注皮瓣微循环的体内模型”显微外科。

Ichioka, S. et al.: "Clinical use of amrinone(aselective phosphodiesterase III inhibitor) In reconstructive surgery"Plast Reconstr Surg. 108. 1931-1937 (2001)

Ichioka, S. 等人：“氨力农（选择性磷酸二酯酶 III 抑制剂）在重建手术中的临床应用”Plast Reconstr Surg。

Minh TC, Ichioka S, Nakatsuka T, Kawai J, Shibata M, Ando J, Harii K: "Effect of hyperthermic preconditioning on the survival of ischemia-reperfused skin flap: a new skin-flap model in the mouse"J Reconstr Microsurg. 18. 115-119 (2002)

Minh TC、Ichioka S、Nakatsuka T、Kawai J、Shibata M、Ando J、Harii K：“高温预处理对缺血再灌注皮瓣存活的影响：小鼠新皮瓣模型”J Reconstr Microsurg。

Ichioka S, Minh TC, Shibata M, Nakatsuka T, Sekiya N, Ando J, Harii K: "In vivo model for visualizing flap microcirculation of ischemia-reperfusion"Microsurgery. 22. 304-310 (2002)

Ichioka S、Minh TC、Shibata M、Nakatsuka T、Sekiya N、Ando J、Harii K：“用于可视化缺血再灌注皮瓣微循环的体内模型”显微外科。

Ichioka, S. et al.: "Microvascular and interstitial PO(2)measurements in rat akeletal muscle by phosphorescence quenching"J Appi Physiol. 91. 321-327 (2001)

Ichioka, S. 等人：“通过磷光猝灭对大鼠骨骼肌进行微血管和间质 PO(2) 测量”J Appi Physiol。