Development of micro CT for in VIVO

用于in VIVO的显微CT的开发

• 批准号: 15390570
• 负责人: ARAI Yoshinori
• 金额: $ 5.76万
• 依托单位: Matsumoto Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390570/
• 关键词: X-ray; CT; Animal; micro CT; in VIVO; 動物

ObjectivesThe aim of this paper is to report a newly developed micro-computed tomography for in vivo use.MethodsThe system was composed of a micro focus X-ray tube and an image intensifier (I.I.) that rotated around the object stage. A Guinea pig and a rat were examined. The anesthetized animal was set on the secure object stage. Images of the head of the Guinea pig and the tibia/knee joint of the rat were taken. In addition, a specimen of the rat tail was taken. The reconstruction and the image viewing were carried out using i-view software (J. Morita MFG, Kyoto, Japan).ResultsThe voxel matrix was 512x512x384. The voxel sizes ranged from 10μm x 10μm x 10μm to 100μm x 100μm x 100μm. The exposure time was 17 s, and the reconstruction time was 150 s. The head of the Guinea pig and the tibia/knee joint of the rat were observed clearly under 100-μm and 30-μm voxels, respectively. The trabecular bone of the tail was also observed clearly under the 10-μm voxel.ConclusionsThe newly developed micro-computed tomography makes it possible to obtain images of anesthetized animals which set on a secure object stage. Clear bone images of the small animals could be obtained within a short operation time.

目的本文的目的是报告一种新开发的用于体内使用的微型计算机断层扫描。方法该系统由微焦点X射线管和围绕物台旋转的图像增强器（I.I.）组成。对一只豚鼠和一只大鼠进行了检查。将麻醉的动物放置在安全的物体台上。拍摄豚鼠头部和大鼠胫骨/膝关节的图像。此外，还采集了大鼠尾巴的样本。使用i-view软件（J. Morita MFG，Kyoto，Japan）进行重建和图像查看。结果体素矩阵为512x512x384。体素尺寸范围从 10μm x 10μm x 10μm 到 100μm x 100μm x 100μm。曝光时间为17秒，重建时间为150秒。分别在 100 μm 和 30 μm 体素下清晰地观察到豚鼠的头部和大鼠的胫骨/膝关节。在10微米体素下也可以清楚地观察到尾部的小梁骨。结论新开发的微型计算机断层扫描技术使得获得放置在安全物台上的麻醉动物的图像成为可能。手术时间内即可获得小动物清晰的骨骼图像。