Small animal tomographic imager for luminescence, fluorescence and CT

用于发光、荧光和 CT 的小动物断层成像仪

• 批准号: 507944703
• 金额: --
• 依托单位: Charité - Universitätsmedizin Berlin
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/507944703?language=en
• 关键词: Small; animal; tomographic; imager; luminescence

Imaging methods for small animal models are an essential method in basic biomedical research to describe physiological and pathophysiological processes at the organismic level in relation to anatomy, metabolism, signal transduction and the interaction of different organs and cell types. Conventional optical imaging methods in 2D are limited both in their anatomical significance, the number of parameters that can be described and the fact that no real quantification is possible. With the planar 2D methods, the results are falsified to a considerable extent by scattering and absorption. As a result, precise localization and exact determination of intensities (and thus determination of the absolute concentration of probe molecules) are not possible. A tomographic small animal imager for luminescence, fluorescence and CT allows non-invasive imaging of the anesthetized, living organism (typically mouse) with considerable penetration depth, with microCT-corrected, improved 3D reconstruction of the optical signals and thus high accuracy of location and intensity data. The integrated microCT also provides an important anatomical reference for the observation of the probes via fluorescence and bioluminescence. Bioluminescence has also developed into a standard procedure for growth and therapy monitoring in preclinical oncology and for cell tracking in immunological questions. The research groups involved in the application deal with issues that require this state-of-the-art technology in order to be able to conduct competitive research. Specifically, this technology is required, for example, to localize cell migration through the precise 3D representation of the organism or to check the successful targeting of tumor cells by molecular probes through precise organ segmentation. The required methodical bases are already established in the working groups and an optical system, which enables this detection, is accordingly required.

在基础生物医学研究中，小动物模型的成像方法是描述与解剖、代谢、信号转导以及不同器官和细胞类型相互作用有关的生物体水平上的生理和病理生理过程的基本方法。传统的2D光学成像方法在解剖学意义、可描述的参数数量以及无法进行真正量化的事实方面都受到限制。对于平面2D方法，由于散射和吸收，结果在很大程度上是错误的。因此，不可能精确定位和准确确定强度(从而确定探针分子的绝对浓度)。用于发光、荧光和CT的断层扫描小动物成像仪允许对麻醉的活体(通常是小鼠)进行非侵入性成像，具有相当大的穿透深度，并经过MicroCT校正，改进了光学信号的3D重建，从而提高了位置和强度数据的准确性。集成的MicroCT也为通过荧光和生物发光观察探针提供了重要的解剖学参考。生物发光也已发展成为临床前肿瘤学中生长和治疗监测以及免疫学问题中细胞追踪的标准程序。参与申请的研究小组处理的问题需要这种最先进的技术才能进行有竞争力的研究。具体地说，这项技术是需要的，例如，通过生物体的精确3D表示来定位细胞迁移，或者通过精确的器官分割来检查分子探针对肿瘤细胞的成功靶向。工作组已经建立了所需的方法基础，因此需要一个能够进行这种检测的光学系统。