权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Study on mechanical properties and invivo of bone filling materials with chitosan as a bonding agent which had net structure

以壳聚糖为粘结剂的网状结构骨填充材料力学性能及体内研究

基本信息

批准号：
15390600
负责人：
ITO Michio
金额：
$ 5.89万
依托单位：
Matsumoto Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

A self-hard type bone filling material has been developed by mixing the chitosan sol and mixed powder ; the former chitosan sol was prepared by dissolving 85% de-acetyl chitosan (C85) and 99% de-acetyl chitosan (C99) with organic acids, while the latter was a mixture of hydroxyapatite particles, CaO powder, and ZnO powder. The above mentioned synthetic bone filling material was compressed to form a testing sample for compression tests. Test samples were stored in physiological solution (which is equivalent to a body fluid) for 1,7,28, and 56 days prior to compression tests. Amounts of Ca ion, P ion, Zn ion dissolved from samples into the physiological solution were analyzed and measured. The progressive changes in pH value of the solution were also monitored and measured. The bone conduction was also investigated when the chitosan sols with C85 and C99 materials were placed at a rat tibia.Main results are as follows ; The bone filling material using C99 did not show a remarkable decrease in compressive strength, but it exhibited a continuous but smaller decrease by immersion time than the filling material using C85. The pH value of bone filling material using C99 was higher than that using C85 material. The dissolved Ca ion from the bone filling material is no different between these two type of materials. The animal test results suggested that, although the bone filling materials using C85 did not show any bone conduction, the bone filling material using C99 indicated a bone conduction and a substitution with bony structure.

将壳聚糖溶胶与混合粉混合制成自硬型骨填充材料，前者由85%脱乙酰壳聚糖(C85)和99%脱乙酰壳聚糖(C99)用有机酸溶解而成，后者由羟基磷灰石颗粒、CaO粉和氧化锌粉混合而成。将上述合成骨充填材料进行压缩，形成压缩试验试件。在压缩试验之前，将测试样品储存在生理溶液(相当于体液)中1、7、28和56天。分析测定了样品中钙离子、磷离子、锌离子在生理溶液中的溶出量。还监测和测量了溶液的pH值的渐进性变化。将含有C85和C99材料的壳聚糖溶胶放置于大鼠胫骨，结果表明：与C85材料相比，C99材料的骨填充材料的抗压强度没有明显的下降，但随着浸泡时间的延长，抗压强度有持续的下降，但下降幅度较小。C99骨充填材料的pH值高于C85骨充填材料。骨充填材料中溶解的钙离子在两种材料中没有差别。动物实验结果表明，虽然使用C85的骨填充材料没有表现出任何骨传导，但使用C99的骨填充材料表现出骨传导和具有骨结构的替代。