Study on mechanical properties and invivo of bone filling materials with chitosan as a bonding agent which had net structure

将壳聚糖溶胶与混合粉混合制成自硬型骨填充材料；前者是85%去乙酰化壳聚糖（C85）和99%去乙酰化壳聚糖（C99）与有机酸溶解制备的壳聚糖溶胶，后者是羟基磷灰石颗粒、CaO粉和ZnO粉的混合物。将上述合成骨填充材料压缩成试样进行压缩试验。测试样品在压缩测试前在生理溶液（相当于体液）中储存1、7、28和56天。分析测定了样品中Ca离子、P离子、Zn离子溶解到生理溶液中的量。同时对溶液pH值的变化进行了监测和测量。研究了C85和C99壳聚糖溶胶对大鼠胫骨骨传导的影响。主要研究结果如下：使用C99的骨填充材料抗压强度下降不明显，但与使用C85的填充材料相比，使用C99的骨填充材料的抗压强度随浸泡时间的增加而下降，但下降幅度较小。使用C99的骨填充材料的pH值高于使用C85的材料。骨填充材料中溶解的钙离子在这两种材料中没有区别。动物实验结果表明，虽然使用C85的骨填充材料不表现出骨传导，但使用C99的骨填充材料表现出骨传导和骨结构的替代。

A self-hard type bone filling material has been developed by mixing the chitosan sol and mixed powder ; the former chitosan sol was prepared by dissolving 85% de-acetyl chitosan (C85) and 99% de-acetyl chitosan (C99) with organic acids, while the latter was a mixture of hydroxyapatite particles, CaO powder, and ZnO powder. The above mentioned synthetic bone filling material was compressed to form a testing sample for compression tests. Test samples were stored in physiological solution (which is equivalent to a body fluid) for 1,7,28, and 56 days prior to compression tests. Amounts of Ca ion, P ion, Zn ion dissolved from samples into the physiological solution were analyzed and measured. The progressive changes in pH value of the solution were also monitored and measured. The bone conduction was also investigated when the chitosan sols with C85 and C99 materials were placed at a rat tibia.Main results are as follows ; The bone filling material using C99 did not show a remarkable decrease in compressive strength, but it exhibited a continuous but smaller decrease by immersion time than the filling material using C85. The pH value of bone filling material using C99 was higher than that using C85 material. The dissolved Ca ion from the bone filling material is no different between these two type of materials. The animal test results suggested that, although the bone filling materials using C85 did not show any bone conduction, the bone filling material using C99 indicated a bone conduction and a substitution with bony structure.