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Expression of Function in Pollen Protoplasts

花粉原生质体的功能表达

基本信息

批准号：
63540549
负责人：
ITO Michio
金额：
$ 1.34万
依托单位：
Shizuoka University (1989-1990)Nagoya University (1988)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63540549/
关键词：
Pollen Protoplast Lilium longiflorum pollen tube elongation cell fusion

项目摘要

Mature pollen of Angiosperms germinate on the stegma of the same species and elongates pollen tubes, in which the generative nucleus divides into two sperm nuclei capable of fertilization. When protoplasts were isolated from mature pollen which have such two specific functions, after the regeneration of cell wall in culture,how expresses the functions in such regenerated pollen protoplasts was the project of this study.1. The isolation of protoplasts from mature pollen were examined in various species of Angiosperms, and only 4 species within 43 species of dicotyledons, but 27 species within 31 species of monocotyledons were successful. The success depends on the size of the germination pole.2. In Lilium longiflorum, protoplasts from mature pollen were isolated at high frequencies (70-90%). After the regeneration of cell wall in culture, more than 80% of them were able to elongate pollen tube on solid germination medium. The regenerated pollen did also germinate on lily stigmata and the pollen tubes elongated into style through the ovule, but the fertilization and the seed formation were not ascertained.3. When the pollen protoplasts of the lily were electrically induced to fuse, the fused protoplasts elongated essentially one pollen tube, regardless of the cell number of fusion. Fusion of pollen protoplasts between the lily and the other species also resulted in the elongation of one pollen tube. In the pollen tubes, the generative nuclei of both species divided into two sperm nuclei. On stigma of each species, the fused pollen of heterokaryon germinated and elongated pollen tube into style.4. The function of the pollen tube elongation expressed in fused protoplats between lily pollen and tulip mesophyl cells. In the tubes, the dividing figures of the generative nucleus of the lily and the removal of chloroplasts of the somatic cells were observed one day after germination on solid medium.

被子植物的成熟花粉在同种的柱头上萌发，花粉管伸长，其中的生殖核分裂成两个能够受精的精核。当从成熟花粉中分离出具有这两种特定功能的原生质体后，培养细胞壁再生后，如何在再生的花粉原生质体中表达其功能是本研究的课题。 1．在各种被子植物中对成熟花粉原生质体的分离进行了检测，双子叶植物43种中只有4种成功，单子叶植物31种中27种成功。成功与否取决于发芽极的大小。2．在长花百合中，从成熟花粉中分离出原生质体的频率较高（70-90%）。培养细胞壁再生后，80%以上的细胞能够在固体萌发培养基上延长花粉管。再生花粉也在百合柱头上萌发，花粉管通过胚珠伸长成花柱，但受精和种子形成尚未确定。3．当百合的花粉原生质体被电诱导融合时，无论融合的细胞数量如何，融合的原生质体基本上伸长了一个花粉管。百合和其他物种之间花粉原生质体的融合也导致一个花粉管的伸长。在花粉管中，两个物种的生殖核都分为两个精子核。各物种的柱头上，异核体融合的花粉萌发，花粉管伸长成花柱。4．百合花粉和郁金香叶肉细胞融合原生平板中表达的花粉管伸长功能。在试管中，在固体培养基上发芽一天后观察百合生殖核的分裂情况和体细胞叶绿体的去除。