Experimental Studies in vivo for chitosan-bonded bone filling material.

壳聚糖粘合骨填充材料的体内实验研究。

• 批准号: 10671850
• 负责人: ITO Michio
• 金额: $ 2.11万
• 依托单位: Matsumoto Dental Univ.
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671850/
• 关键词: Bone filling material; chitin-chitosan; hydroxyapatite; DNA; ALPase; 生体吸収性

Three kinds of bone filling materials were prepared by combining powder, including CaO, CaSiOィイD23ィエD2 and hydroxyapatite (HAP), and chitin and chitosan. The proportion of CaO, CaSiOィイD23ィエD2 and HAP was 4.5%, 6.0% and 89.5%. This powder 0.54g(A), 0.67g(B) and 0.80gc were kneaded with chitin and chitosan sol 2.2g and hardened, respectively. These materials were evaluated in the experiment animal and osteoblastic cells. The purpose of this study was to discuss which materials were most desirable for the bone filling materials. In an animal experiment, tissue reactions were similar in each material and were characterized by granulation tissue formation with inflammation. In the osseous tissue, repairs at defected sites (B and C) and direct relationship between material A and revealed that DNA contents and alkaline phosphatase activity in material A were significantly higher that those in control. Results of this study chitosan sol 2.2g, was most effective for the bone formation.

将CaO、CaSiO纳米颗粒D23、CaSiO纳米颗粒D2和羟基磷灰石（HAP）粉末与甲壳素、壳聚糖粉末复合，制备了3种骨填充材料。CaO、CaSiO_2、D_23、D_2和HAP的含量分别为4.5%、6.0%和89.5%。将0.54 g（A）、0.67 g（B）和0.80 g（C）的粉末分别与2.2 g甲壳素和壳聚糖溶胶捏合并硬化。在实验动物和成骨细胞中对这些材料进行评价。本研究的目的是讨论哪些材料是最理想的骨填充材料。在动物实验中，每种材料的组织反应相似，特征为肉芽组织形成伴炎症。在骨组织中，缺损部位（B和C）的修复与材料A和材料C之间的直接关系表明，材料A的DNA含量和碱性磷酸酶活性显著高于对照组。本研究结果以壳聚糖溶胶2.2g为最佳成骨效果。

项目成果

森厚二: "キチン・キトサンに関する研究その9.キチン・キトサンを結合材とする骨補填材のCaSiO_3添加に関する実験的研究"日本口腔インプラント学会誌. 12. 185-192 (1999)

Atsushi Mori：“甲壳素和壳聚糖的研究第9部分。使用甲壳素和壳聚糖作为粘合材料在骨移植材料中添加CaSiO_3的实验研究”日本口腔种植学会杂志12. 185-192（1999）。

森厚二: "ケイ酸カルシウムを添加した骨補填材の培養骨芽細胞様細胞による評価"第33回日本歯科理工学会抄録集. 18. 71 (1999)

Atsushi Mori：“使用培养的成骨细胞样细胞评价含有硅酸钙的骨移植材料”，第33届日本牙科科学与工程学会会议记录，18. 71（1999）。