On the production, fusion, and function of pollen protoplasts

对不同高等植物成熟花粉原生质体的酶解分离、培养融合及其细胞壁再生后的功能表达进行了研究。被子植物花粉原生质体的分离。从100多种双子叶植物中分离出8种原生质体，从31种单子叶植物中分离出27种原生质体。将长花百合（Lilium longiflorum）的花粉原生质体培养2 d后置于柱头上。花粉管通过子房向雌蕊伸长，形成种子。在百合原生质体融合过程中，细胞壁的再生、花粉管的伸长和生殖细胞的分裂均表现正常。花粉管数通常为1个，营养核在花粉管的前面。原生质体在不同种间融合时，通常从融合体中伸出一个花粉管。在花粉与中叶质原生质体融合过程中，花粉功能在细胞壁再生后才得以表达。利用气动颗粒枪成功地在长花百合花粉中表达了b-葡糖醛酸酶基因。

Enzymatic isolation of protoplasts from mature pollen in various species of higher plants, their culture and fusion and their functional expression after the regeneration of cell wall were studied.1.Isolation of protoplasts from pollen in Angiosperms. The protoplasts were obtained from 8 dicotyledon species within more than 100 species tried, whereas 27 within 31 species in monocotyleadons.2.In Lilium longiflorum, pollen protoplasts were cultured for 2 days and placed on the stigma. Pollen tubes elongated into their pistil through ovary, and resulted in the seed formation.3.In fusion of lily protoplasts, the regeneration of cell wall, elongation of pollen tubes and the division of generative cells showed normal behavior. The number of of pollen tubes was usally one, and vegetative nuclei preceeded in the tubes. In protoplast fusion between defferent species, usually one pollen tube elongated from the fusion body.4.In fusion between pollen and mesohyll protoplasts, the function as pollen was expressed after the regeneration of cell wall.5.A b-glucuronidase gene was successfully expressed in pollen of Lilium longiflorum using a pneumatic particle gun.