Inhibition of dental plaque formation and determination of anti-cariogenic effect by DNA vaccine

DNA疫苗抑制牙菌斑形成及防龋效果测定

基本信息

  • 批准号:
    15390639
  • 负责人:
  • 金额:
    $ 8.77万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2003
  • 资助国家:
    日本
  • 起止时间:
    2003 至 2004
  • 项目状态:
    已结题

项目摘要

Glucosyltransferases(GTFs) from Streptococcus mutans synthesis the adhesive glucans from sucrose and are important virulence factor of dental caries. The purposes of this investigation are : 1)Determination of the immunogenicity of GTF domains to select target region of anti-caries DNA vaccine, 2)Construct DNA vaccine against the candidate domain, 3)Analysis of anti-cariogenic effect of the DNA vaccine.GTFs commonly possesses three (variable, catalytic, and glucan-binding) domains. Antigenicity of each domain was predicted by Paker's method, then the recombinant plasmids carrying these domains of GTFs from S.mutans MT8148 were constructed. Western blot analysis revealed that immunogenicity of the catalytic and glucan-binding regions was low. On the other hand, the variable region was thought to possess immunogenicity.DNA vaccine plasmid containig the variable region of GTF was constructed using pAFC3 which was carrying the adeno virus promoter. The vaccine plasmid was transfected into 293 cells and the expression of mRNA was confirmed by Northen blot analysis.To the next step, we will determine the anti-cariogenic activity of the DNA vaccine in in vitro and in vivo (animal) experiments.
变形链球菌的葡萄糖基转移酶(GTF)是由蔗糖合成粘附性葡聚糖的酶,是龋病的重要毒力因子。本研究的目的是:1)测定GTF结构域的免疫原性,筛选防龋DNA疫苗的靶区; 2)针对候选结构域构建DNA疫苗; 3)分析DNA疫苗的防龋效果。用Paker法预测各结构域的抗原性,构建了含这些结构域的重组质粒。Western印迹分析表明,催化和葡聚糖结合区的免疫原性低。另一方面,GTF的可变区具有免疫原性,利用携带腺病毒启动子的pAFC 3构建了含有GTF可变区的DNA疫苗质粒。将构建的DNA疫苗质粒转染293细胞,Northen blot检测其mRNA的表达,并进行体外和动物体内抗龋实验。

项目成果

期刊论文数量(14)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Method for rapid identification of oral streptococci by PCR using 16S-23S ribosomal RNA intergenic spacer gene
  • DOI:
    10.1016/s0917-2394(05)70051-3
  • 发表时间:
    2005-01-01
  • 期刊:
  • 影响因子:
    0.8
  • 作者:
    Hoshino, Tomonori;Izumi, Tomohiro;Fujiwara, Taku
  • 通讯作者:
    Fujiwara, Taku
PCR detection and identification of oral streptococci in saliva samples using gtf genes
IMMUNOGENICITY OF GTF DOMAINS FROM STR. MUTANS FOR DNA VACCINE CANDIDATE
STR. GTF 结构域的免疫原性
Immunogenicity of gtf domains from Str.mutans for DNA vaccine candidate
候选 DNA 疫苗中变形链球菌 gtf 结构域的免疫原性
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FUJIWARA Taku其他文献

FUJIWARA Taku的其他文献

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{{ truncateString('FUJIWARA Taku', 18)}}的其他基金

Collection and Construction of database for physiological and physical support of children
儿童生理体质支持数据库的收集与建设
  • 批准号:
    24390463
  • 财政年份:
    2012
  • 资助金额:
    $ 8.77万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Removal of pharmaceuticals in secondary effluent using magnetic particle containing TiO_2-Zeolite composite catalyst under solar light
太阳光下磁性颗粒TiO_2-沸石复合催化剂去除二级出水中的药物
  • 批准号:
    23656332
  • 财政年份:
    2011
  • 资助金额:
    $ 8.77万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Investigation about dental caries vaccine using human-type anti-GTF antibody
人源抗GTF抗体龋齿疫苗的研究
  • 批准号:
    23659968
  • 财政年份:
    2011
  • 资助金额:
    $ 8.77万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Development of a novel system consisting of catch crop cultivation and L-lactate fermentation of harvested biomass for nitrate groundwater pollution control and resource recovery
开发一种由农作物种植和收获生物质 L-乳酸发酵组成的新型系统,用于硝酸盐地下水污染控制和资源回收
  • 批准号:
    21310054
  • 财政年份:
    2009
  • 资助金额:
    $ 8.77万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Basic study for development of DNA vaccine against dental caries
龋齿DNA疫苗研制的基础研究
  • 批准号:
    13470449
  • 财政年份:
    2001
  • 资助金额:
    $ 8.77万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of mutant generation system of Streptococcus mutans, and analysis of the functional domain of glucosyltransferases using the mutant.
开发变形链球菌突变体产生系统,并利用该突变体分析葡萄糖基转移酶的功能域。
  • 批准号:
    09672104
  • 财政年份:
    1997
  • 资助金额:
    $ 8.77万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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口腔细菌变形链球菌通过血栓形成促进肿瘤转移
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  • 批准号:
    10749982
  • 财政年份:
    2023
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Interactions of Streptococcus mutans in Supragingival Biofilms
龈上生物膜中变形链球菌的相互作用
  • 批准号:
    10666551
  • 财政年份:
    2022
  • 资助金额:
    $ 8.77万
  • 项目类别:
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阐明口腔微生物组中变形链球菌的整合和结合元件的调节和传播
  • 批准号:
    10604661
  • 财政年份:
    2022
  • 资助金额:
    $ 8.77万
  • 项目类别:
Interactions of Streptococcus mutans in Supragingival Biofilms
龈上生物膜中变形链球菌的相互作用
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Lrg丙酮酸吸收系统在变形链球菌环境适应中的作用
  • 批准号:
    10645783
  • 财政年份:
    2022
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    $ 8.77万
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Characterization and membrane-biogenesis of Streptococcus mutans magnesium transporters
变形链球菌镁转运蛋白的表征和膜生物发生
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Characterization and membrane-biogenesis of Streptococcus mutans magnesium transporters
变形链球菌镁转运蛋白的表征和膜生物发生
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    10353066
  • 财政年份:
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Streptococcus mutansが関与する脳血管疾患発症機序の解明
阐明变形链球菌脑血管疾病的发病机制
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    22K10352
  • 财政年份:
    2022
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    $ 8.77万
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变形链球菌二腺苷酸环化酶:预防龋齿的一个有希望的目标
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    10330368
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