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Researches on Genetic Resources of Native Animals and it's Related Wild Animals in Bhutan

不丹本土动物及相关野生动物遗传资源研究

基本信息

批准号：
15405033
负责人：
YAMAMOTO Yoshio
金额：
$ 7.62万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15405033/
关键词：
Bhutan Genetic resources Livestock cattle pig chicken suncus DNA

项目摘要

This research survey is planned to be a part of research surveys on Asian native livestock. The Kingdom of Bhutan is situated on the southern slopes of the Eastern Himalayas. It is a land locked between China in the north and India to the east, south and west. Its physical features are characterized by high, rugged mountains and an intricate network of deep valleys and rejected an invader from neighboring countries. As a results of preliminary survey at July of 2003, it was confirmed that an abundance of genetic resources of native livestocks and its related wild species are ranged through the mountains and border areas.First research survey : June 15-30,2004. Participant : Y.Yamamoto, K.Nomura, M.Nishibori, T.Yamagata, Y.Takahashi, M.Kurachi and two Bhutanese scientists1)Survey areas : middle and southern part of Bhutan (Bumthang, Zhemgang, Sarpan, and Phunakha)2)No.of surveyed animals : cattle:23、water buffalo:18、goat:24、chicken:40、mithan:3、junglefowl:0、suncus:8Second research survey … More : August 2-30,2004. Participant : Y.Yamamoto, T.Namikawa, K.Tsunoda, K.Tanaka, K.Kinoshita, M.Kurachi, Y.Miyazaki and two Bhutanese scientists.1)Survey areas : east and west part of Bhutan (Haa, Paro, Chhukha, Trashigang, Lhuentse, Mongar)2)No.of surveyed animals : cattle:81、water buffalo:6、horse:44,sheep:76、goat:0、pig:29、chicken:64、mithan:11、junglefowl:0、suncus:21No.of morphological recorded animals except above mentioned : cattle:1534、water buffalo:36、horse:471、sheep:145、goat:203、pig:45、mithan:16、chicken:487、dog:187、cat:1483)Survey and/or analysis items : record for coat and feather color, body measurement, electrophoresis for blood proteins, blood group typing, DNA extraction for even-toed ungulates, collection for egg albumen.{Results for survey and analysis}All scheduled survey except junglefowl was successfully finished. Gene frequencies for each species were estimated from the results for morphological traits, blood groups, protein polymorphisims. DNA analysis are still continueing at each laboratory in Japan. Less

本研究调查计划作为亚洲本地牲畜研究调查的一部分。不丹王国位于东喜马拉雅山脉的南坡上。这是一块北接中国，东、南、西接印度的土地。它的物理特征是高而崎岖的山脉和错综复杂的深谷网络，并拒绝了来自邻国的入侵者。2003年7月的初步调查结果证实，在山区和边境地区分布着丰富的本地家畜及其相关野生物种遗传资源。第一次研究调查：2004年6月15-30日。参与者：yyyamamoto， k.o nomura, m.nishbori, t.o yamagata, y.o takahashi， M.Kurachi和两位不丹科学家1)调查区域：不丹中部和南部（Bumthang, Zhemgang， Sarpan和Phunakha）被调查的动物：牛：23头，水牛：18头，山羊：24头，鸡：40头，美洲豹：3头，丛林鸮：0头，美洲豹：8头，第二次研究调查…更多：2004年8月2-30日。参与者：yamamoto， namikawa, tsunoda, tanaka, kinoshita, kurachi， yymiyazaki和两位不丹科学家。1)调查区域：不丹东部和西部（Haa, Paro, Chhukha, Trashigang, Lhuentse, Mongar）在被调查的动物中：牛：81头，水牛：6头，马：44头，绵羊：76头，山羊：0头，猪：29头，鸡：64头，美洲豹：11头，丛林雉：0头，美洲豹：21头。调查和/或分析项目：皮毛和羽毛颜色记录、身体测量、血液蛋白电泳、血型分型、偶趾有蹄类动物DNA提取、鸡蛋蛋白收集。{调查分析结果}除junglefowl外，所有预定调查均已成功完成。根据形态特征、血型、蛋白质多态性的结果估计每个物种的基因频率。日本各实验室的DNA分析仍在继续。少