Analysis of chicken QTL by using DNA markers and chromosome map

利用DNA标记和染色体图谱分析鸡的QTL

• 批准号: 10306017
• 负责人: YAMAMOTO Yoshio
• 金额: $ 20.26万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10306017/
• 关键词: chicken; QTL; DNA marker; linkage analysis; chromosome map; reference family; QTR

[Establishment of reference family for QTL analysis and characterize the economical traits of chicken lines] : 2,930 individuals of F2 generation were established from Oh-shamo and White Leghorn mating. As the economical traits, body weight, shank length, egg production, egg weight, meat color, crude fat, crude protein, amino acid, pH, and myoglobin were measured. [Development of DNA markers and its typing] : Eighty functional genes were mapped on chromosome map by using direct R-banding FISH method. No.1, 2, 4, and 5 of chicken chromosomes had high homology with human chromosomes. Chicken Z chromosome was found to be high homology with No.5 and 9 of human chromosomes. Out of 800 microsatellite DNA developed, 314 microsatellite DNA were informative in our reference family. [Analysis of immune-associated gene] : Recombinant protein from chicken IL-6 gene was identified and its activity was confirmed. An expression of mRNA of IL-6 gene was confirmed in GST cell line induced by IPTG stimulation.

[QTL分析参考家系的建立和鸡系经济性状的表征]：通过Oh-shamo和白来航交配建立了F2代2,930个体。作为经济性状，测定体重、腿长、产蛋量、蛋重、肉色、粗脂肪、粗蛋白、氨基酸、pH、肌红蛋白。 [DNA标记的开发及其分型]：采用直接R显带FISH方法将80个功能基因定位在染色体图谱上。鸡的1、2、4、5号染色体与人类染色体具有较高的同源性。鸡Z染色体被发现与人类5号、9号染色体高度同源。在开发的 800 个微卫星 DNA 中，有 314 个微卫星 DNA 在我们的参考家族中提供了信息。 [免疫相关基因分析]：鉴定了鸡IL-6基因的重组蛋白并证实了其活性。在IPTG刺激诱导的GST细胞系中证实了IL-6基因的mRNA表达。

项目成果

M. Nishibori, et al.: "Utility of three restriction fragment length polymorphism probes for genotyping of the chicken major histocompatibility complex class IV region"Poultry Science. 79. 305-311 (2000)

M. Nishibori 等人：“利用三种限制性片段长度多态性探针对鸡主要组织相容性复合体 IV 类区域进行基因分型”《家禽科学》。

M.Mishibori, et al.: "Genetic relationships among genus Gallus based on mitochondorial and genome DNA polymorphisims"In : Proceeding of 27^<th> ISAG. 15 (2000)

M.Mishibori等人：“基于线粒体和基因组DNA多态性的原鸡属之间的遗传关系”，见：第27届ISAG论文集。

T.Suzuki,Y.Matsuda et al.: "Cytogenetic mapping of 31 functional genes on chicken chromosomes by direct R-banding FISH"Cytogenetics and Cell Genetics. 87. 32-40 (1999)

T.Suzuki、Y.Matsuda 等人：“通过直接 R 显带 FISH 对鸡染色体上 31 个功能基因进行细胞遗传学作图”《细胞遗传学和细胞遗传学》。

T.Yoshimura, et al.: "Molecular analysis of avian circadian clock genes"Mol.Brain Res.. 78. 207-215 (2000)

T.Yoshimura 等：“鸟类生物钟基因的分子分析”Mol.Brain Res.. 78. 207-215 (2000)

T.Suzuki et al.,: "Cytogenetic assignment of 29 functional genes to chicken microchromosomes by FISH"Cytogenetics and Cell Genetics. 87. 233-237 (1999)

T.Suzuki 等人：“通过 FISH 对鸡微染色体进行 29 个功能基因的细胞遗传学分配”《细胞遗传学和细胞遗传学》。