Analysis of chicken QTL by using DNA markers and chromosome map

[鸡QTL分析参考家系的建立及品系经济性状鉴定]：以欧沙莫和白来航鸡为材料，建立了2,930个F_2代个体。测定体重、胫长、产蛋量、蛋重、肉色、粗脂肪、粗蛋白、氨基酸、pH和肌红蛋白等经济性状。[DNA标记的发展及其分型]：采用直接R显带FISH方法在染色体图谱上定位了80个功能基因。鸡1号、2号、4号和5号染色体与人类染色体同源性较高。鸡Z染色体与人类5号和9号染色体高度同源。在已开发的800个微卫星DNA中，有314个微卫星DNA在我们的参考家系中具有信息性。[免疫相关基因分析]：鉴定鸡IL-6基因重组蛋白并证实其活性。经IPTG刺激后，GST细胞表达IL-6基因。

[Establishment of reference family for QTL analysis and characterize the economical traits of chicken lines] : 2,930 individuals of F2 generation were established from Oh-shamo and White Leghorn mating. As the economical traits, body weight, shank length, egg production, egg weight, meat color, crude fat, crude protein, amino acid, pH, and myoglobin were measured. [Development of DNA markers and its typing] : Eighty functional genes were mapped on chromosome map by using direct R-banding FISH method. No.1, 2, 4, and 5 of chicken chromosomes had high homology with human chromosomes. Chicken Z chromosome was found to be high homology with No.5 and 9 of human chromosomes. Out of 800 microsatellite DNA developed, 314 microsatellite DNA were informative in our reference family. [Analysis of immune-associated gene] : Recombinant protein from chicken IL-6 gene was identified and its activity was confirmed. An expression of mRNA of IL-6 gene was confirmed in GST cell line induced by IPTG stimulation.