Analysis of chicken QTL by using DNA markers and chromosome map

【QTL分析参考家系的建立和鸡品系经济性状的表征】：通过Oh-shamo和白色来航鸡交配，建立F2代2,930个个体。作为经济性状，测定体重、胫长、产蛋量、蛋重、肉色、粗脂肪、粗蛋白、氨基酸、pH和肌红蛋白。[DNA标记的建立及其分型]：采用直接R-显带FISH方法，将80个功能基因定位在染色体图谱上。鸡的第1、2、4、5号染色体与人的同源性较高。鸡的Z染色体与人的5号和9号染色体有很高的同源性。在800个微卫星DNA中，314个微卫星DNA在我们的参考家庭中提供了信息。【免疫相关基因分析】：对鸡IL-6基因重组蛋白进行鉴定，并确认其活性。IPTG诱导GST细胞系表达IL-6基因。

[Establishment of reference family for QTL analysis and characterize the economical traits of chicken lines] : 2,930 individuals of F2 generation were established from Oh-shamo and White Leghorn mating. As the economical traits, body weight, shank length, egg production, egg weight, meat color, crude fat, crude protein, amino acid, pH, and myoglobin were measured. [Development of DNA markers and its typing] : Eighty functional genes were mapped on chromosome map by using direct R-banding FISH method. No.1, 2, 4, and 5 of chicken chromosomes had high homology with human chromosomes. Chicken Z chromosome was found to be high homology with No.5 and 9 of human chromosomes. Out of 800 microsatellite DNA developed, 314 microsatellite DNA were informative in our reference family. [Analysis of immune-associated gene] : Recombinant protein from chicken IL-6 gene was identified and its activity was confirmed. An expression of mRNA of IL-6 gene was confirmed in GST cell line induced by IPTG stimulation.