Analyses of functional roles of TLS polymerases using transgenic mice

为了研究跨损伤合成(TLS)在哺乳动物细胞中的功能作用，我们在Pol ETA、Pol iota或Pol kappa单、双或三缺陷小鼠胚胎成纤维细胞(MEF)中建立了许多表达聚合酶活性缺陷或Rev1相互作用缺陷突变DNA聚合酶ETA(PolETA)的小鼠胚胎成纤维细胞系。利用这些MEF细胞，我们研究了紫外线敏感性以及紫外线照射下的突变频率。我们的结果表明，在没有Pol ETA的情况下，存在一条容易出错的TLS途径，即通过Rev1使用Pol kappa。

In order to examine the functional roles of translesion synthesis (TLS) in mammalian cells, we have established many mouse embryonic fibroblast cell lines expressing polymerase activity-defective or Rev1 interaction-defective mutant DNA polymerase eta (Pol eta) in Pol eta, Pol iota or Pol kappa single-, double- or triple-deficient mouse embryonic fibroblasts (MEFs). Using these MEF cells, we investigated UV sensitivity as well as mutation frequency upon UV irradiation. Our results suggest that there is an error-prone TLS pathway that uses Pol kappa via Rev1 in the absence of Pol eta.