Development of novel gastrointestinal cancer diagnostic system based on large epige-nome analysis.

通过对结直肠癌细胞表观基因组和转录组的分析，我们发现了一些在结直肠癌中表观遗传沉默的microRNA基因。通过对结直肠癌癌前病变和早期结直肠癌组织的分析，我们发现了新的DNA甲基化改变，这可能是早期发现结直肠癌的潜在生物标志物。此外，我们还发现了结直肠癌癌前病变中肿瘤表面微结构、病理结果和分子改变之间的新关系。我们对原发性胃癌(GC)的背景胃粘膜进行了表观基因组分析，发现了新的DNA甲基化改变，可能是新的GC危险标记。最后，通过对原发性肝细胞癌进行表观基因组分析，我们发现了新的DNA甲基化改变，这可能是新的肝细胞癌标志物。

Through analyzing epigenome and transcriptome in colorectal cancer (CRC) cells, we identified a number of microRNA genes which were epigenetically silenced in CRC. By analyzing colorectal premalignant lesions and early CRC tissues, we identified novel DNA methylation alterations, which could be potential biomarkers for early detection of CRCs. Moreover, we discovered a novel relationship between tumor surface microstructure, pathological findings and molecular alterations in the colorectal premalignant lesion. We carried out epigenome analysis the background gastric mucosa of primary gastric cancer (GC), and identified novel DNA methylation changes which could be novel GC risk markers. Finally, by performing epigenome analysis in primary hepatocellular carcinoma (HCC), we discovered novel DNA methylation changes, which could be novel HCC markers.