Detection of Novel Amyloid-Associated Protein by Monoclonal Antibody and its Gene Analysis

新型淀粉样蛋白相关蛋白的单克隆抗体检测及其基因分析

基本信息

  • 批准号:
    01570363
  • 负责人:
  • 金额:
    $ 1.41万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1989
  • 资助国家:
    日本
  • 起止时间:
    1989 至 1990
  • 项目状态:
    已结题

项目摘要

We have found that monoclonal antibody AM34 (IgGl) recognizes a novel antigen which exists in amyloid deposits in various organs of the rheumatoid arthritis.In addition, anti-beta-protein monoclonal antibodies (TB1, TB2, TB3, TB4 and TB5) were generated from the fusion of mouse myeloma X63-Ag8.653 cells with splenocytes obtained from a BALB/c mouse that had been immunized with a synthetic peptide of beta-protein consisting of 24 amino acids. All these antibodies bound specifically to the amyloid changes of senile plaque and amyloid angiopathy of the brain tissues of patients with Alzheimer's disease (AD) or with senile dementia Alzheimer type (SDAT) , but not at all to protein in the normal brain or other tissues by the immunoperoxidase method. Formic acid pretreatment of brain tissue enhanced this reactivity. Positive reactions were clearly visible even in small deposits of plaque, senile plaque crown amyloid and amyloid deposits of capillary vessels under this condition.Western blot analysis revealed that monoclonal antibodies TB2 and TB3 reacted mainly with 116 kD band when extract of SDAT brain was used. They failed to react, however, with 116kD band when extract of normal brain derived from patients with other diseases was used. Two dimensional electrophoresis revealed that the 116 kD band migrated at around pl8.5. MoAbs TB1, TB2 and TB4 strongly reacted with the 1st part (7 amino acids near to N-terminus) of the synthetic peptide (24 amino acids) of the beta-protein, which consists of hydrophilic amino acids.
我们发现,在类风湿性关节炎的不同器官中淀粉样蛋白沉积中存在一种新的抗原,该抗体可识别一种新的抗原。此外,用24个氨基酸组成的β蛋白合成肽免疫BALB/c小鼠获得的脾细胞与小鼠骨髓瘤X63-Ag8.653细胞融合,产生抗β蛋白的单抗(TB_1、T_2、T_3、B_4和T_5)。这些抗体均与阿尔茨海默病(AD)或老年性痴呆阿尔茨海默型(SDAT)患者脑组织淀粉样斑块和淀粉样血管病变特异结合,而与正常脑或其他组织中的蛋白质完全不结合。甲酸对脑组织的预处理增强了这种反应性。在此条件下,即使在少量斑块、老年斑、冠状淀粉样蛋白和毛细血管淀粉样物沉积中也可见明显的阳性反应。Western印迹分析表明,SDAT脑提取液中单抗TB2和Tb3主要与116kD条带反应。然而,当使用从其他疾病患者的正常脑提取液时,他们未能反应出116kD条带。双向电泳法显示,116kD条带在p8.5左右发生迁移。MoAbTb1、Tb2和Tb4与由亲水性氨基酸组成的β蛋白合成肽(24个氨基酸)的第一部分(N端附近的7个氨基酸)发生强烈反应。

项目成果

期刊论文数量(8)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
斎藤 丹羽子: "β蛋白に対するモノクロ-ナル抗体を用いたアルツハイマ-病の免疫学的検討" 札幌医学雑誌. (1991)
Niwako Saito:“使用针对 β 蛋白的单克隆抗体进行阿尔茨海默病的免疫学研究”札幌医学杂志(1991 年)。
  • DOI:
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  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Saito, N. et al.: "Immunological analysis of Alzheimer's disease with monoclonal antibodies to beta protein" Sapporo Medical Journal. (1991)
Saito, N. 等人:“使用β蛋白单克隆抗体对阿尔茨海默病进行免疫学分析”札幌医学杂志。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Imai,K.: "Cloning of AM34 antigen gene associated with amyloid deposit" PNAS USA. (1990)
Imai,K.:“与淀粉样蛋白沉积相关的 AM34 抗原基因的克隆”美国 PNAS。
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    0
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IMAI Kohzoh其他文献

IMAI Kohzoh的其他文献

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{{ truncateString('IMAI Kohzoh', 18)}}的其他基金

Identification of specific cancer stem cell markers for diagnosis
鉴定用于诊断的特定癌症干细胞标记物
  • 批准号:
    24650632
  • 财政年份:
    2012
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Development of novel gastrointestinal cancer diagnostic system based on large epige-nome analysis.
基于大表观基因组分析的新型胃肠道癌症诊断系统的开发。
  • 批准号:
    23240129
  • 财政年份:
    2011
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Scientific Support Programs for Cancer Research Grant-in-Aid for Scientific Research on Innovative Areas
癌症研究科学支持计划 创新领域科学研究资助计划
  • 批准号:
    221S0001
  • 财政年份:
    2010
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
Molecular mechanisms of epigenetic alterations in gastrointestinal cancer and application to diagnosis and therapy
胃肠癌表观遗传改变的分子机制及其在诊断和治疗中的应用
  • 批准号:
    17109008
  • 财政年份:
    2005
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (S)
Immunotherapy using humanized monoclonal antibody
使用人源化单克隆抗体的免疫疗法
  • 批准号:
    17016060
  • 财政年份:
    2005
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
Microsatellite instability and aberrant methylation in gastrointestinal cancer and its application to molecular target therapy
胃肠道肿瘤微卫星不稳定性和异常甲基化及其在分子靶向治疗中的应用
  • 批准号:
    13854016
  • 财政年份:
    2001
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (S)
Immunelogical diagnosis of MSI-positive gastrointestinal cancer and application for cancer vaccine
MSI阳性胃肠癌的免疫学诊断及癌症疫苗的应用
  • 批准号:
    12470124
  • 财政年份:
    2000
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Analysis of molecular mechanism of premalignant lesion of the stomach using genomic instability and new mucin gene.
利用基因组不稳定性和新粘蛋白基因分析胃癌前病变的分子机制。
  • 批准号:
    11557043
  • 财政年份:
    2000
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
AnalysIs of molecular mechanism of gastric pre-cancerous lesions by using new mucin genes
新粘蛋白基因分析胃癌前病变的分子机制
  • 批准号:
    09470141
  • 财政年份:
    1997
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of a supersensitive detection method for pancreas cancer associated antigen and tumor suppressor gene by using immuno-PCR.
利用免疫PCR开发胰腺癌相关抗原和抑癌基因的超灵敏检测方法。
  • 批准号:
    07557046
  • 财政年份:
    1995
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

相似海外基金

Supporting characters may go to the heart of amyloidogenesis: Amyloid associated protein world
配角可能涉及淀粉样蛋白生成的核心:淀粉样蛋白相关蛋白世界
  • 批准号:
    24790557
  • 财政年份:
    2012
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
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