AnalysIs of molecular mechanism of gastric pre-cancerous lesions by using new mucin genes

新粘蛋白基因分析胃癌前病变的分子机制

• 批准号: 09470141
• 负责人: IMAI Kohzoh
• 金额: $ 2.18万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470141/
• 关键词: mucin genes; gastric pre-cancerous lesions; Intestinal metaplasia; gastric cancer; Barett esohagus; 分子機構解析

Our previous study showed that a new mucin antigen A3D4 was expressed at the protein level in gastric cancer and intestinal metaplasia associated with cancer, but not in normal gastric mucosa and intestinal metaplasia with chronic gastritis. To verify if the antigen is of use for diagnostic purpose, we performed an immunohistochemical study using a large number of gastric tissue specimens obtained by endoscopic biopsy. Antigen A3D4 was expressed in adenoma, especially intestinal type with high frequency and in intestinal metaplasia in the vicinity of adenoma. Of note, about 20% of intestinal metaplasia with chronic gastritis cases demonstrated to be positive for antigen A3D4, where only part of the deep portion of glands were faintly immunostained. These data suggest that antigen A3D4-positive intestinal metaplasia cases may be grouped into a high risk group for gastric adenoma and cancer.It is known that MUC2 mucin is expressed in goblet cells of intestinal metaplasia, but not in normal gastric mucosa. We revealed that MUC2 was expressed in regenerative epithelia and gastric cancer, especially intestinal type, in addition to intestinal metaplasia. It will be important to examine the expression of MUC2 in the background mucosa of gastric cancer except for intestinal metaplasia. We then showed the expression of sulfo-Lewis A, which was shown expressed on MUC2 mucin core protein, in Barrette*s esophagus and esophageal adenocarcinoma by using a monoclonal antibody 91.9H.Thus, these two antigens, MUC2 and sulfo-Lewis A may be of use for analyzing the precancerous lesions of the stomach and esophagus, respectively.

我们先前的研究表明，新的粘蛋白抗原A3D4在胃癌和癌症肠道上的蛋白质水平上表达与癌症相关的肠道抗原，但在正常的胃粘膜和肠道肠道上没有慢性胃炎。为了验证抗原是否用于诊断目的，我们使用大量通过内窥镜活检获得的大量胃组织样本进行了免疫组织化学研究。抗原A3D4在腺瘤中表达，尤其是腺瘤附近的肠道类型高频率和肠道上的肠道。值得注意的是，大约有20％的肠道化生具有慢性胃炎病例的抗原A3D4阳性，其中只有部分深层对腺体的一部分被微弱的免疫染色。这些数据表明，抗原A3D4阳性肠道化生病例可能会分为胃腺瘤和癌症的高风险组。知道MUC2粘蛋白在肠道上层状的杯状细胞中表达，但在正常胃粘膜中不显示。我们揭示了MUC2在再生性上皮癌和胃癌，尤其是肠道类型中表达，除了肠道化生型。除了肠道化生症外，检查MUC2在胃癌背景粘膜中的表达将很重要。 We then showed the expression of sulfo-Lewis A, which was shown expressed on MUC2 mucin core protein, in Barrette*s esophagus and esophageal adenocarcinoma by using a monoclonal antibody 91.9H.Thus, these two antigens, MUC2 and sulfo-Lewis A may be of use for analyzing the precancerous lesions of the stomach and esophagus, respectively.

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