Regulation of chromatin structures and hematopoietic cell differentiation by architectural transcription factors Bach1 and Bach2

结构转录因子 Bach1 和 Bach2 对染色质结构和造血细胞分化的调节

• 批准号: 11470030
• 负责人: IGARASHI Kazuhiko
• 金额: $ 9.47万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470030/
• 关键词: Transcription factor; nuclear structure; BTB domain; LCR; cell differentiation; cell death; クロマチン; インスレーター; 転写; Maf

This study focused on the function of transcription factors Bach1 and Bach2 in regulation of chromatin structures at the molecular levels as well as their roles in hematopoietic cell differentiation. They possess the BTB/POZ domain that has been implicated in regulation of chromatin structures. To understand the biochemical basis for the BTB/POZ domain function, we searched for proteins that interact with the Bach1 BTB/POZ domain by yeast two hybrid sereening. Isolated candidates include a protein that regulates actin structure, transcritional coregulators, and structural proteins. In addition, we found that Bach2 regulates oxidative stress-induced cell death. Thus, further analysis of the interactions may pave a way to understand functional link among regulation of transcription, chromatin structures, and cell differentiation.

这项研究的重点是转录因子BACH1和BACH2在分子水平上调节染色质结构及其在造血细胞分化中的作用。它们具有与调节染色质结构有关的BTB/POZ结构域。为了了解BTB/POZ结构域功能的生化基础，我们搜索了通过酵母两种杂交递归与Bach1 BTB/POZ结构域相互作用的蛋白质。孤立的候选物包括调节肌动蛋白结构，转换核心调节剂和结构蛋白的蛋白质。此外，我们发现Bach2调节氧化应激诱导的细胞死亡。因此，对相互作用的进一步分析可以铺平一种理解转录，染色质结构和细胞分化调节之间的功能联系的方法。

项目成果

ItoH, K., et al.: "Keap1 represses nuclear activation of antioxidant responsive elements by Nrf2 through binding to the amino-terminal Neh2 domain."Genes and Development. 13. 76-86 (1999)

ItoH, K., 等人：“Keap1 通过与氨基末端 Neh2 结构域结合，抑制 Nrf2 对抗氧化反应元件的核激活。”基因与开发。

Kanezaki, R., Toki, T., Yokoyama, M., Yomogida, K., Sugiyama, K., Yamamoto, M., Igarashi, K., and Ito, E.: "Transcription factor BACH1 is recruited to the nucleus by its novel alternative spliced isoform."J.Biol.Chem.. 276. 7278-7284 (2001)

Kanezaki, R.、Toki, T.、Yokoyama, M.、Yomogida, K.、Sugiyama, K.、Yamamoto, M.、Igarashi, K. 和 Ito, E.：“转录因子 BACH1 被招募到细胞核

Ikeda, Y., Sugawara, A., Taniyama, Y., Uruno, A., Igarashi, K., Arima, S., Ito, S., Takeuchi, K.: "Suppression of rat thromboxane synthase gene transcription by peroxisome-proliferator-activated receptor-ganna via an intercation with NF-E2 releted factor

池田，Y.，菅原，A.，谷山，Y.，乌鲁诺，A.，五十岚，K.，有马，S.，伊藤，S.，竹内，K.：“过氧化物酶体抑制大鼠血栓素合酶基因转录

Kobayashi, A., et al.: "Molecular cloning and functional characterization of a new Cap'n'collar family transcription factor Nrf3."Journal of Biological Chemistry. 274. 6443-6452 (1999)

Kobayashi, A. 等人：“新型 Capncollar 家族转录因子 Nrf3 的分子克隆和功能表征。”生物化学杂志。

Kanezaki,R.: "Transcription factor BACH1 is recruited to the nucleus by its novel alternative spliced isoform."J.Biol.Chem.. 276. 7278-7284 (2001)

Kanezaki, R.：“转录因子 BACH1 通过其新颖的选择性剪接亚型被招募到细胞核。”J.Biol.Chem.. 276. 7278-7284 (2001)