Regulation of gene transcription and nuclear structure by architectural transcription factors

结构转录因子对基因转录和核结构的调节

• 批准号: 13470024
• 负责人: IGARASHI Kazuhiko
• 金额: $ 8万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470024/
• 关键词: chromatin; transcription factor; globin; LCR; BTB domain

We found :1. Binding of Bach1 to the globin LCR is regulated by heme, the prothetic group of hemoglobin.2. Heme oxygenase-1 (HO-1) is another physiological target gene of Bach1.3. Heme regulates binding of Bach1 to the LCR of HO-1 gene through direct binding to Bach1. The BTB domain of Bach1 is essential for the Bach1 binding to the HO-1 LCR.4. Bach1 regulates HO-1 gene expression through mechanisms that are distinct from regulation of histone acetylation-deacetylation.5. Bach2 accumulates within the PML nuclear bodies to repress gene expression there.6. The BTB domain of Bach2 is essential for the targeting of Bach2 to PML bodies.

我们发现：1. Bach 1与球蛋白LCR的结合受血红素（血红蛋白的辅基）调节.血红素加氧酶-1（HO-1）是Bach1.3的另一个生理靶基因。血红素通过与Bach 1的直接结合来调节Bach 1与HO-1基因LCR的结合。Bach 1的BTB结构域对于Bach 1与HO-1 LCR的结合是必需的。Bach 1通过不同于组蛋白乙酰化-去乙酰化的机制调控HO-1基因的表达。Bach 2在PML核体中积累以抑制那里的基因表达。Bach 2的BTB结构域对于Bach 2靶向PML体是必不可少的。

项目成果

Ogawa, K.: "Heme mediates de-repression of Maf recognition element through direct binding to transcription repressor Bach1"EMBO J.. 20. 2835-2843 (2001)

小川，K.：“血红素通过直接结合转录抑制因子 Bach1 介导 Maf 识别元件的去抑制”EMBO J.. 20. 2835-2843 (2001)

Sun, J.: "Hemoprotein Bach 1 regulates enhancer availability of heme oxygenase-1 gene"EMBO J.. 21. 5216-5224 (2002)

Sun, J.：“血红素蛋白 Bach 1 调节血红素加氧酶-1 基因的增强剂可用性”EMBO J.. 21. 5216-5224 (2002)

Tanabe, O.: "Fission yeast homologues of the B' subunit of protein phosphatase 2A : multiple roles in mitotic cell division and functional interaction with calcineurin"Genes Cells. 6. 455-474 (2001)

Tanabe, O.：“蛋白磷酸酶 2A B 亚基的裂殖酵母同源物：在有丝分裂细胞分裂和与钙调神经磷酸酶功能相互作用中的多种作用”Genes Cells。

Katsuoka, F.: "Small Maf compound mutants display central nervous system neuronal degeneration, aberrant transcription, and Bach protein mislocalization coincident with myoclonus and abnormal startle response"Mol. Cell Biol.. 3. 1163-1174 (2003)

Katsuoka, F.：“小 Maf 复合突变体表现出中枢神经系统神经元变性、异常转录和巴赫蛋白错误定位，与肌阵挛和异常惊吓反应一致”Mol。

Sun, J.: "Promoter of Mouse Transcription Repressor bach1 Is Regulated by Sp1 and Trans-Activated by Bach1"J. Biochem.. 130. 385-392 (2001)

Sun, J.：“小鼠转录抑制子 bach1 的启动子受 Sp1 调节并由 Bach1 反式激活”J.