Regulation of chromatin structures during hematopoietic cell differentiation

这项研究集中在造血细胞中染色质结构的组织和细胞谱系特异性调节。有几种转录因子与造血特异基因的基因位点控制区结合。为了了解它们的作用，我们培育了缺乏转录因子基因bach1或bach2的突变小鼠。进一步分析这些小鼠的表型变化可能为理解转录调控、染色质结构和细胞分化之间的功能联系铺平道路。在这些实验中，我们还使用酵母双杂交筛选与Bach1或Bach2相互作用的蛋白质。分离的候选蛋白包括调节肌动蛋白结构的蛋白质、转录辅助调节蛋白和结构蛋白，这表明它们在染色质结构的调节中具有意义。

This study focused on the tissu-and cell lineage-specific regulation of chromatin structures in hematopoietic cells. There are several transcription factors that bind to the locus control region of hematopoietic-specific genes. To understand their roles, we generated mutant mice lacking the transcription factor genes bach1 or bach2. Further analysis of the phenotypic changes of these mice may pave a way to understand functional link among regulation of transcription, chromatin structures, and cell differentiation. Along these experiments, we also screened for proteins that interact with Bach1 or Bach2 using yeast two hybrid screen. Isolated candidates include proteins that regulate actin structure, transcritional co-regulators, and structural proteins, suggesting their implication in regulation of chromatin structures.