Molecular investigation on the hepatic caicinogenesis-resistant model animals
肝脏抗癌模型动物的分子研究
基本信息
- 批准号:11557090
- 负责人:
- 金额:$ 4.03万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1999
- 资助国家:日本
- 起止时间:1999 至 2001
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The Rb gene was isolated as the causative gen of retinoblastoma. The Rb protein has an anti-proliferative action and has been known to be lost in many tumors. We made the transgenic mice in the liver of which the Rb protein was overexpressed in order to investigate the in vivo roles of Rb protein. Line A has 11 copies/haploid of the transgenes and line B has 4 copies/haploid. Consistently, a large amount of Rb protein was detected in the liver of line A transgenic mice and a small amount of it was detected in that of line B transgenic mice. The two lines of mice developed normally and the livers were normal in size and histology.We investigated whether the Rb transgenic mice showed resistance to chemical carcinogenesis. We inject diethylnitrosamine into the peritoneal cavity at the ages of 4 and 6 weeks and treated phenobarbital in drinking water for 35 weeks. After the experiment, the mice were sacrificed to make histological sections for counting the numbers of hepatocellular carcinoma and hepatic nodule. In control mice, a large number of nodules and several hepatocellular carcinoma were developed In contrast, the number of nodules was greatly reduced and no hepatocellular carcinomas were detected in both lines of Rb transgenic mice. The anti-carcinogenic effect of Rb gene was demonstrated in the experiments using the 4 week old mice and the 6 week old mice. These results indicate that the HNF1-Rb gene unit might be useful for the preventive medicine in the near future.
Rb基因是视网膜母细胞瘤的致病基因。Rb蛋白具有抗增殖作用,并且已知在许多肿瘤中丢失。为了研究Rb蛋白在体内的作用,我们在肝脏中制备了Rb蛋白过表达的转基因小鼠。系A具有11个拷贝/单倍体的转基因,系B具有4个拷贝/单倍体。A系转基因小鼠肝脏中检测到大量Rb蛋白,B系转基因小鼠肝脏中检测到少量Rb蛋白。两品系小鼠发育正常,肝脏大小和组织学正常,我们研究了Rb转基因小鼠是否对化学致癌具有抵抗力。我们在4和6周龄时将二乙基亚硝胺注入腹腔,并在饮用水中处理苯巴比妥35周。实验结束后处死小鼠,制作组织切片,计数肝癌和肝结节的数目。在对照组小鼠中,大量的结节和几个肝细胞癌的发展,相比之下,结节的数量大大减少,没有检测到肝细胞癌在两个系的Rb转基因小鼠。在4周龄和6周龄小鼠实验中证实了Rb基因的抗癌作用。这些结果表明,HNF 1-Rb基因单元可能在预防医学上有应用价值。
项目成果
期刊论文数量(62)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
三浦直行: "明らかになってきたフォークヘッド遺伝子の器官形成における役割"蛋白質核酸酵素. 46. 26-35 (2001)
Naoyuki Miura:“叉头基因在器官形成中的作用”蛋白质核酸酶。 46. 26-35 (2001)
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- 影响因子:0
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Komatsu M.: "Copper transporting P-type adenosine triphosphatase (ATP7B) is associated with Cisplatin resistance."Cancer Res.. 60. 1312-1316 (2000)
Komatsu M.:“铜转运 P 型腺苷三磷酸酶 (ATP7B) 与顺铂耐药性相关。”Cancer Res.. 60. 1312-1316 (2000)
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- 影响因子:0
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- 通讯作者:
Wang, T. et al.: "Inhibition effects of di(2-ethylhexyl)phthalate on mouse liver lysosomal Vacuolar H+-ATPase"J. Cell. Biochem. 81. 295-303 (2001)
Wang, T.等:“邻苯二甲酸二(2-乙基己基)酯对小鼠肝脏溶酶体液泡H-ATP酶的抑制作用”J。
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- 影响因子:0
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三浦直行: "22q11欠失症候群の遺伝子医学"遺伝子医学. 5. 204-211 (2001)
Naoyuki Miura:“22q11缺失综合征的基因医学”《基因医学》5. 204-211 (2001)。
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- 影响因子:0
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Berg,D.: "Changes of copper transporting proteins and ceruloplasmin in the lentiform nuclei in primary adult-onset dystonia."Ann.Neurol.. 47. 827-830 (2000)
Berg,D.:“原发性成人肌张力障碍中豆状核中铜转运蛋白和铜蓝蛋白的变化。”Ann.Neurol.. 47. 827-830 (2000)
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- 影响因子:0
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MIURA Naoyuki其他文献
MIURA Naoyuki的其他文献
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{{ truncateString('MIURA Naoyuki', 18)}}的其他基金
Generation of the HCV-infectable mouse-an animal model for inflammation cancer
HCV感染小鼠的产生——炎症性癌症动物模型
- 批准号:
24659603 - 财政年份:2012
- 资助金额:
$ 4.03万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Generation of mice in which the mouse hepatocytes are replaced with the human hepaocytes and its application
人肝细胞替代小鼠肝细胞的小鼠产生及其应用
- 批准号:
19390347 - 财政年份:2007
- 资助金额:
$ 4.03万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular mechanism of hepatic carcinogenesis and hepatocyte apoptosis in the Rb transgenic mice
Rb转基因小鼠肝癌发生及肝细胞凋亡的分子机制
- 批准号:
15390393 - 财政年份:2003
- 资助金额:
$ 4.03万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular machanism of the MFH-1 gene in, the aoortic arch formation, Skeletogenesis and kidney formation
MFH-1基因在主动脉弓形成、骨骼发生和肾脏形成中的分子机制
- 批准号:
11694239 - 财政年份:1999
- 资助金额:
$ 4.03万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular investigation on the fulminant hepatitis-resistant model animals
暴发性肝炎耐药模型动物的分子研究
- 批准号:
10470253 - 财政年份:1998
- 资助金额:
$ 4.03万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
ROLES OF THE MFH-1 AND WT1 GENES IN KIDNEY DEVELOPMENT
MFH-1 和 WT1 基因在肾脏发育中的作用
- 批准号:
09044252 - 财政年份:1997
- 资助金额:
$ 4.03万 - 项目类别:
Grant-in-Aid for international Scientific Research
Roles of the MFH-1 gene and WT 1 gene in kidney development
MFH-1 基因和 WT 1 基因在肾脏发育中的作用
- 批准号:
08044238 - 财政年份:1996
- 资助金额:
$ 4.03万 - 项目类别:
Grant-in-Aid for international Scientific Research
GENERATION AND APPLICATION OF THE MODEL MICE WHICH SHOW RESISTANCE TO RENAL CARCINOGENESIS
抗肾癌小鼠模型的产生及应用
- 批准号:
08557089 - 财政年份:1996
- 资助金额:
$ 4.03万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Lsolation and characterization of the Brain Forkhead gene
脑叉头基因的分离和表征
- 批准号:
05680592 - 财政年份:1993
- 资助金额:
$ 4.03万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Molecular Cloning of Rat Hepatocyte Nuclear Factor 1 Gene and Analysis of Its Promotor
大鼠肝细胞核因子1基因的分子克隆及其启动子分析
- 批准号:
02670108 - 财政年份:1990
- 资助金额:
$ 4.03万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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