Roles of the MFH-1 gene and WT 1 gene in kidney development

MFH-1 基因和 WT 1 基因在肾脏发育中的作用

基本信息

  • 批准号:
    08044238
  • 负责人:
  • 金额:
    $ 1.02万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for international Scientific Research
  • 财政年份:
    1996
  • 资助国家:
    日本
  • 起止时间:
    1996 至 无数据
  • 项目状态:
    已结题

项目摘要

We isolated the MFH-1 gene as one of the forkhead gene family in 1993. The family are growing and more than 20 genes are known in mice. The MFH-1 gene is expressed at the somitic mesoderm and later expressed strongly in the sclerotome. It is also expressed in the developing metanephros and later on in the glomerulus after birth.We made knockout mice of the MFH-1 gene by homologous recombination in the ES cells. First, we cloned the mouse genomic MFH-1 gene and determined its genomic structure. We found that it has no introns. We made a targeting vector by replacing the exon with neo-resistance cassette and introduced it into ES cells by electroporation. Out of 480 neomycin- and gancyclovir-resistant clones, 6 were identified to be homologous recombinants. We made chimera mice by injecting them into a blastocyst and obtained heterozygotic mice by mating chimera mice and wild B6 mice. The heterozygotes were asymptomatic. However, homozygotes were dead within ten minutes after birth.Homozygotes had abnormalities in several tissues. Here we focused the phenotype of kidneys. The size of kidneys in homozygotes was half that of the wild-type. Histologically the glomerulus, renal tubular cells and blood vesssels were present normally. But dilated renal calces were seen in homozygotes.Next, we cloned the human genomic MFH-1 gene and determined its nucleotide sequence. We found that human MFH-1 protein is 501 amino acids long and the amino acid sequence of the forkheah domain is identical in human and mouse. Identity of the mouse and human MFH-1 protein is 94%.We also made monoclonal antibodies against the human MFH-1 protein. Using thses antibodies, we found that the MFH-1 protein is expressed in chondrosarcoma, osteosarcoma and Wilms tumor cell lines.
我们于1993年分离到MFH-1基因作为叉头基因家族的一员。这个家族还在不断壮大,目前已知老鼠体内有20多个基因。MFH-1基因在体裂中胚层表达,随后在硬核组强烈表达。它也在发育中的后肾和出生后的肾小球中表达。我们在胚胎干细胞中通过同源重组制备MFH-1基因敲除小鼠。首先,我们克隆了小鼠基因组MFH-1基因并确定了其基因组结构。我们发现它没有内含子。我们用新抗性盒代替外显子制成靶向载体,并通过电穿孔将其导入ES细胞。在480个新霉素和更昔洛韦耐药克隆中,6个被鉴定为同源重组。我们将嵌合体小鼠注射到囊胚中制备,并将嵌合体小鼠与野生B6小鼠交配得到杂合子小鼠。杂合子无症状。然而,纯合子在出生后10分钟内死亡。纯合子在一些组织中有异常。这里我们关注的是肾脏的表型。纯合子的肾脏大小是野生型的一半。组织学上肾小球、肾小管细胞和血管正常。但在纯合子中可见扩张的肾盏。接下来,我们克隆了人类基因组MFH-1基因并确定了其核苷酸序列。结果表明,人MFH-1蛋白全长501个氨基酸,其forkheah结构域的氨基酸序列与小鼠相同。小鼠和人MFH-1蛋白的同源性为94%。我们还制备了针对人MFH-1蛋白的单克隆抗体。利用这些抗体,我们发现MFH-1蛋白在软骨肉瘤、骨肉瘤和Wilms肿瘤细胞系中表达。

项目成果

期刊论文数量(15)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Hirasawa,F.: "The effect of silver administration on the biosynthesis and molecular propartics of rat ceruloplasmin" Biochim.Biophys.Acta. (in press).
Hirasawa,F.:“银给药对大鼠铜蓝蛋白生物合成和分子原配体的影响”Biochim.Biophys.Acta。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Masuda Y.: "Platelet-derived growth factor B-chain homodimer suppressing a tonic-clonic convulsion of seizure-prone E1 mice" Biochim.Biophys.Res.Commun.233. 60-63 (1996)
Masuda Y.:“血小板衍生生长因子 B 链同二聚体抑制癫痫易发 E1 小鼠的强直阵挛惊厥”Biochim.Biophys.Res.Commun.233。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Masuda,Y.: "Platelet-derived growth factor B-chain homodimer suppressing a tonic-clonic convulsion fo seizure-prone El mouse." Biochim. Biophys. Res. Commun.223. 60-63 (1996)
Masuda,Y.:“血小板衍生生长因子 B 链同二聚体抑制癫痫易发性 El 小鼠的强直阵挛性惊厥。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Miura,N.: "Isolation of the mouse and human MFH-1 (Mesenchyme Fork Head-1) genes reveals conscrvation of their gene and protein structures." Genomics. (in press).
Miura,N.:“小鼠和人类 MFH-1(间充质叉头 1)基因的分离揭示了它们基因和蛋白质结构的保守性。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Yasui,O.: "Isolation of oval cells from Long-Evans Cinnamon rats and their transformation into hepatocytes in vivo in the rat liver" Hepatology. 25. 329-334 (1997)
Yasui,O.:“从 Long-Evans Cinnamon 大鼠中分离卵圆细胞及其在大鼠肝脏中转化为肝细胞”肝病学。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

MIURA Naoyuki其他文献

MIURA Naoyuki的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('MIURA Naoyuki', 18)}}的其他基金

Generation of the HCV-infectable mouse-an animal model for inflammation cancer
HCV感染小鼠的产生——炎症性癌症动物模型
  • 批准号:
    24659603
  • 财政年份:
    2012
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Generation of mice in which the mouse hepatocytes are replaced with the human hepaocytes and its application
人肝细胞替代小鼠肝细胞的小鼠产生及其应用
  • 批准号:
    19390347
  • 财政年份:
    2007
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular mechanism of hepatic carcinogenesis and hepatocyte apoptosis in the Rb transgenic mice
Rb转基因小鼠肝癌发生及肝细胞凋亡的分子机制
  • 批准号:
    15390393
  • 财政年份:
    2003
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular machanism of the MFH-1 gene in, the aoortic arch formation, Skeletogenesis and kidney formation
MFH-1基因在主动脉弓形成、骨骼发生和肾脏形成中的分子机制
  • 批准号:
    11694239
  • 财政年份:
    1999
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular investigation on the hepatic caicinogenesis-resistant model animals
肝脏抗癌模型动物的分子研究
  • 批准号:
    11557090
  • 财政年份:
    1999
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular investigation on the fulminant hepatitis-resistant model animals
暴发性肝炎耐药模型动物的分子研究
  • 批准号:
    10470253
  • 财政年份:
    1998
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
ROLES OF THE MFH-1 AND WT1 GENES IN KIDNEY DEVELOPMENT
MFH-1 和 WT1 基因在肾脏发育中的作用
  • 批准号:
    09044252
  • 财政年份:
    1997
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
GENERATION AND APPLICATION OF THE MODEL MICE WHICH SHOW RESISTANCE TO RENAL CARCINOGENESIS
抗肾癌小鼠模型的产生及应用
  • 批准号:
    08557089
  • 财政年份:
    1996
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Lsolation and characterization of the Brain Forkhead gene
脑叉头基因的分离和表征
  • 批准号:
    05680592
  • 财政年份:
    1993
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Molecular Cloning of Rat Hepatocyte Nuclear Factor 1 Gene and Analysis of Its Promotor
大鼠肝细胞核因子1基因的分子克隆及其启动子分析
  • 批准号:
    02670108
  • 财政年份:
    1990
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似海外基金

Elucidation of the relationship between life span and carnitine biosynthetic capacity in metallothionein knockout mice.
阐明金属硫蛋白基因敲除小鼠的寿命与肉碱生物合成能力之间的关系。
  • 批准号:
    23K10907
  • 财政年份:
    2023
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Pathological analysis focused on impairment of unmyelinated fibers in striatal medium spiny neuron-specific Tsc1 knockout mice
病理分析重点关注纹状体中型多棘神经元特异性 Tsc1 敲除小鼠无髓纤维损伤
  • 批准号:
    22K07560
  • 财政年份:
    2022
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
UBR4 Knockout Mice: a New Model of Sudden Unexpected Death in Epilepsy (SUDEP)
UBR4 基因敲除小鼠:癫痫猝死 (SUDEP) 的新模型
  • 批准号:
    565264-2021
  • 财政年份:
    2021
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Alexander Graham Bell Canada Graduate Scholarships - Master's
Analysis of the mechanisms of Acinetobacter infection in klotho knockout mice
Klotho基因敲除小鼠不动杆菌感染机制分析
  • 批准号:
    21K08516
  • 财政年份:
    2021
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Mechanistic Basis of Circadian Clocks in Bmal1 Knockout Mice
Bmal1 基因敲除小鼠生物钟的机制基础
  • 批准号:
    10399594
  • 财政年份:
    2021
  • 资助金额:
    $ 1.02万
  • 项目类别:
Mechanistic Basis of Circadian Clocks in Bmal1 Knockout Mice
Bmal1 基因敲除小鼠生物钟的机制基础
  • 批准号:
    10208370
  • 财政年份:
    2021
  • 资助金额:
    $ 1.02万
  • 项目类别:
Generation of Novel Osteolineage VHL Conditional Knockout Mice to Study B Cell Microenvironments
生成新型骨谱系 VHL 条件敲除小鼠以研究 B 细胞微环境
  • 批准号:
    10368064
  • 财政年份:
    2021
  • 资助金额:
    $ 1.02万
  • 项目类别:
Characterization of OGFRL1 Knockout Mice
OGFRL1 敲除小鼠的表征
  • 批准号:
    10193252
  • 财政年份:
    2021
  • 资助金额:
    $ 1.02万
  • 项目类别:
Characterization of OGFRL1 Knockout Mice
OGFRL1 敲除小鼠的表征
  • 批准号:
    10361562
  • 财政年份:
    2021
  • 资助金额:
    $ 1.02万
  • 项目类别:
Mechanistic Basis of Circadian Clocks in Bmal1 Knockout Mice
Bmal1 基因敲除小鼠生物钟的机制基础
  • 批准号:
    10798455
  • 财政年份:
    2021
  • 资助金额:
    $ 1.02万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了