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Generation of the HCV-infectable mouse-an animal model for inflammation cancer

HCV感染小鼠的产生——炎症性癌症动物模型

基本信息

批准号：
24659603
负责人：
MIURA Naoyuki
金额：
$ 2.41万
依托单位：
Hamamatsu University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2012
资助国家：
日本
起止时间：
2012-04-01 至 2014-03-31
项目状态：
已结题

项目摘要

Experimental plan expected that short promoter has no transcriptional activity to synthesize HCV genome RNA. Unexpectedly, the transgenic mouse showed that they produce HCV genome RNA in hepatocytes and also viral particles into serum. Using the mouse serum from the transgenic mouse, the virus infected Huh7.5.1 cells and produced Core protein in the cytoplasm. This means that the virus was infectious.Next we investigated whether the HCV genome RNA can replicate in the mouse hepatocytes. When the replication inhibitor was injected into the abdomen of the mouse, the RNA copy number was decreased by 30% in the hepatocytes and serum. This result showed that mouse replication factor(s) were competent for viral RNA replication as human factor(s) do.

实验计划预计短启动子不具有合成HCV基因组RNA的转录活性。出乎意料的是，转基因小鼠显示它们在肝细胞中产生HCV基因组RNA，并在血清中产生病毒颗粒。利用转基因小鼠血清，病毒感染Huh7.5.1细胞，在细胞质中产生Core蛋白。这意味着该病毒具有传染性。接下来，我们研究了HCV基因组RNA是否可以在小鼠肝细胞中复制。将复制抑制剂注射到小鼠腹部后，肝细胞和血清中的RNA拷贝数减少30%。结果表明，小鼠复制因子与人复制因子一样具有复制病毒RNA的能力。

项目成果

期刊论文数量（30）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Mechanotransduction, PROX1, and FOXC2 Cooperate to Control Connexin37 and Calcineurin during Lymphatic-Valve Formation

DOI：
10.1016/j.devcel.2011.12.020
发表时间：
2012-02-14
期刊：
DEVELOPMENTAL CELL
影响因子：
11.8
作者：
Sabine, Amelie;Agalarov, Yan;Petrova, Tatiana V.
通讯作者：
Petrova, Tatiana V.

Phosphorylation Regulates FOXC2-Mediated Transcription in Lymphatic Endothelial Cells

DOI：
10.1128/mcb.01387-12
发表时间：
2013-10-01
期刊：
MOLECULAR AND CELLULAR BIOLOGY
影响因子：
5.3
作者：
Ivanov, Konstantin I.;Agalarov, Yan;Petrova, Tatiana V.
通讯作者：
Petrova, Tatiana V.

FOXC2 expression links epithelial-mesenchymal transition and stem cell properties in breast cancer.

DOI：
10.1158/0008-5472.can-12-2962
发表时间：
2013-03-15
期刊：
Cancer research
影响因子：
11.2
作者：
Hollier BG;Tinnirello AA;Werden SJ;Evans KW;Taube JH;Sarkar TR;Sphyris N;Shariati M;Kumar SV;Battula VL;Herschkowitz JI;Guerra R;Chang JT;Miura N;Rosen JM;Mani SA
通讯作者：
Mani SA

The regulation of endogenous retinoic acid level through CYP26B1 is reqired for elevation of palatal shelves

腭架抬高需要通过 CYP26B1 调节内源性视黄酸水平