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Analytical studies on the proteins composed of flagella and basal bodies of swarmers of the brown algae.

褐藻群鞭毛和基体蛋白质的分析研究。

基本信息

批准号：
12440232
负责人：
MOTOMURA Taizo
金额：
$ 8.77万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Swarmers of Heterokonta algae, including brown algae and chrysophyceaen alga Ochromonas have two distinctive flagella, a mastigoneme-bearing long flagellum and a short flagellum. Adding these morphological differences, functions of these flagella are also different. Using synchronized culture of Ochromonas, we found that duplication of the flagellar occurs during G2 - M duding cell cycle, while the formation of daughter basal bodies does during G1 - S. This means that the duplication of flagella of Heterokonta algae is completely dependent to the regulation of the cell cycle. This mechanism is quite similar to the centrosome regulation of animal cells. Various forms of the spindle of Heterokonta algae have been reorted, but we found that basal bodies of flagella are always existed nearby the spindle poles, using antibodies against centrin, gamma-tubulin and microtubules. Also in the case of the brown algae, centrosome functions to the distribution of the cell organella, including chloroplasts, to the daughter cells during mitosis. Recently, it has become clear that centrosome, centriole (=flagellar basal body) and flagellar axomeme affect to the human diseases including cancers. We believe that cell biological studies on the flagellar and centrosomal duplication and the spindle formation using various algae will also attract attentions on the evolution of eukaryotic cells and origin of flagella and centrioles.

异角藻群，包括褐藻和金藻 Ochromonas 有两个独特的鞭毛，一个带有鞭毛的长鞭毛和一个短鞭毛。加上这些形态上的差异，这些鞭毛的功能也不同。通过Ochromonas的同步培养，我们发现鞭毛的复制发生在G2-M期细胞周期，而子代基体的形成则发生在G1-S期。这意味着Heterokonta藻类鞭毛的复制完全依赖于细胞周期的调节。这种机制与动物细胞的中心体调节非常相似。我们对Heterokonta藻类纺锤体的各种形式进行了研究，但我们利用抗中心蛋白、γ-微管蛋白和微管的抗体发现，鞭毛基体始终存在于纺锤体极附近。同样就褐藻而言，中心体在有丝分裂期间将细胞器（包括叶绿体）分配给子细胞。最近，人们已经清楚中心体、中心粒（=鞭毛基体）和鞭毛轴体对包括癌症在内的人类疾病的影响。我们相信，利用各种藻类进行鞭毛和中心体复制以及纺锤体形成的细胞生物学研究也将引起人们对真核细胞进化以及鞭毛和中心粒起源的关注。