Studies on recognition system of male and female gametes and cytoplasmic inheritance of organelles during fertilization and zygote development of algae

采用电子显微镜和分子生物学技术，对褐藻受精过程中叶绿体和线粒体的遗传进行了研究。叶绿体来源于双亲，而来自雄性配子的线粒体DNA在四细胞孢子体中选择性消失。在同卵的裙带菜中，精子叶绿体和线粒体在受精后不久就被溶酶体消化。在hakodatensis中，卵子和精子的细胞器DNA甲基化程度存在明显差异。

Cytoplasmic inheritance of chloroplasts and mitochondria during fertilization of brown algae were studied by electron microscopy and molecular biological techniques using Scytosiphon lomentaria (isogamy), Unidaria pinnatifica and Cystoseira hakodatensis (oogamy). Chloroplasts were derived from both parents in S. lomentaria, while mitochondrial DNA derived from male gametes became selectively disappeared from four-celled sporophytes. In the case of oogamous U. pinnatifida, sperm chloroplasts and mitochondria were digested by lysosome soon after fertilization. In C. hakodatensis, differences of the organellar DNA methylation degrees could be clearly detected between eggs and sperm.