权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Molecular and cytological studies on the cell cycle regulation of marine multinucleate green algae.

海洋多核绿藻细胞周期调控的分子和细胞学研究。

基本信息

批准号：
07640877
负责人：
MOTOMURA Taizo
金额：
$ 1.34万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

Nuclear division in Boergesenia forbesii is very unique in the respect that the nuclear membrane is not fragmented throughout mitosis different from open-spindles in animals and higher plants. In this study, we observed the mitosis in B.forbesii under immunofluorescence microscopy using anti-beta tubulin, anti-centrin and anti-gamma tubulin antibodies. In interphase, microtubules (MTs) radiated around nuclei. One or two pairs of centrioles existed aroud the interphase nucleus. Toward mitosis, perinuclear MTs disappeared and, instead of them, MTs radiated from centrioles. These MT foci separated and migrated to both poles. Simultaneously, tublin molecules started to enter into the nucleus from prophase, and these tubulin molecules formed spindle MTs from poles to chromosomes and from pole to pole, and finally interzonal spindle fibers. Centrin molecules always localized around centrioles through the cell cycle of B.forbesii. On the contrary, gamma-tubulin could not detected around centrioles in interphase. Gamma-tubulin characteristically localized around centrioles from early prophase to early anaphase in mitosis, and they could not be observed around centrioles in late anaphase and telophase. Moreover, gamma-tubulin did not localize in the interzonal spindle fibers. This means that both ends of the interzonal spindle fibers are +end of MTs, not like centrosome.

与动物和高等植物的开放纺锤体不同，羊草的核分裂在整个有丝分裂过程中核膜不是碎裂的，这一点是非常独特的。在本研究中，我们用抗β微管蛋白抗体、抗中心素抗体和抗γ微管蛋白抗体在免疫荧光显微镜下观察了Forbesii有丝分裂。在间期，微管(MT)在细胞核周围辐射。间期核周围有一对或两对中心粒。有丝分裂时，核周MTs消失，取而代之的是中心粒发出的MTs。这些MT灶分离并迁移到两极。同时，微管蛋白分子开始从早期进入细胞核，这些微管蛋白分子形成从极到染色体、从极到极的纺锤形MTS，最后形成带间纺锤体纤维。在Forbesii的整个细胞周期中，Centrin分子始终定位在中心粒周围。相反，间期中心粒周围未检测到γ-微管蛋白。有丝分裂早期至后期，γ-微管蛋白主要分布在中心粒周围，后期和末期中心粒周围未见γ-微管蛋白表达。此外，γ-微管蛋白未定位于带间梭形纤维中。这意味着带间纺锤体纤维的两端都是MTS的+端，而不是中心体。