Advanced multi freedom degree Micromanipulator

先进的多自由度显微操作器

• 批准号: 12450100
• 负责人: KUDOH Ken-ichi
• 金额: $ 9.34万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12450100/
• 关键词: Micromanipulator; Microinjection; Micro actuator; Piezoelectric impact drive; Nuclear Transplant; Piezoelectric actuator; 静電気力; パラレルリンクロボット; 細胞操作

We research is a study about development of a micro manipulator that we use for artificially manipulating plant cell or animal cell. Our aim in this research is to develop a new micro manipulator system which can handle and manipulate one single cell precisely, so for every single cell, we can do injection work accurately and swiftly from every position and angle in order to transplant DNA or nucleus into the cell.Below are our achievements during the research period(1) Micro Pipette Drive Device for Nuclear Transplantation.When we insert micro pipette for nuclear transplantation, the elasticity of the cell become deterrent of the process, and it reduced the efficiency of the work. So. we developed a prototype of super impact micro pipette drive mechanism that is a combination of piezo impact drive mechanism and electromagnetic impact drive mechanism. In the experiment using mouse egg cell, we succeeded to insert the pipette into the cell smoothly without giving damage to the cytoplasm … More . And with the impact power generated by electromagnetic impact drive mechanism, we succeeded to insert the pipette smoothly into other egg cell that is known to have hard cell membrane (for example : pig egg cell) without edge treatment to the tip of the pipette.(2) Animal Egg Cell Handling Mechanism.In the process of denucleation of the egg cell, when the nucleus position is close to the holding pipette, it is necessary to amend the position of the nucleus. This work is very complicated and required high skill of the operator. Therefore, we developed cell rotation mechanism using electrostatic. The egg cell rotates at the speed of 30 deg/s with applied voltage of 4V 500 Hz. we also developed a prototype of mechanism using multiple electrode board and put them into layer. This prototype enables three dimensional cell positioning.(3) Cell Sorter Using Liquid Flow.We developed a prototype with XY-2axis device using voice coil motors. As the result of having done various kinds of experiments with vibration frequency, vibration amplitude, and liquid quantities as parameters, we succeeded to gather intentionally scattered cell using liquid flow.(4) We lent the system we developed to below research institutions, and did experiments for performance evaluation.Kagoshima University, Obihiro University of Agriculture and Veterinary Medicine, Tokyo University of Agriculture, Hirosaki University, St. Mother Hospital. Less

本研究是关于开发一种可用于人工操作植物细胞或动物细胞的微操作器的研究。本研究的目的是开发一种新型的微操作系统，该系统可以精确地操作单个细胞，我们可以从各个位置和角度准确快速地进行注射工作，以便将DNA或细胞核移植到细胞中。以下是我们在研究期间的成果（1）用于核移植的微量移液器驱动装置。当我们插入微量移液器进行核移植时，细胞的弹性成为该过程的阻碍，降低了工作效率。是的研制了一种压电冲击驱动机构和电磁冲击驱动机构相结合的超冲击式微量移液器驱动机构样机。在小鼠卵细胞实验中，我们成功地将移液管顺利插入细胞内，而不损伤细胞质 ...更多信息 .利用电磁冲击驱动机构产生的冲击力，我们成功地将移液管顺利插入其他已知具有硬细胞膜的卵细胞（例如：猪卵细胞），而无需对移液管尖端进行边缘处理。(2)动物卵细胞处理机制：在卵细胞去核过程中，当细胞核的位置接近持液管时，需要修正细胞核的位置。这项工作非常复杂，需要操作人员的高技能。因此，我们开发了利用静电的细胞旋转机制。卵细胞以30度/秒的速度旋转，外加电压为4V 500 Hz。并研制了一种多极板分层机构样机。该原型实现了三维细胞定位。(3)利用液流的细胞分选机。我们开发了一个使用音圈电机的XY-2轴设备的原型。通过以振动频率、振动振幅、液体量为参数进行各种实验，成功地利用液体流收集了有意分散的细胞。(4)我们将开发的系统借给了鹿儿岛大学、带广带广大学、东京农业大学、广崎大学、圣母医院等研究机构，并进行了性能评价实验。少

项目成果

小山 良, 工藤 謙一, 樋口 俊郎, 田中 威づみ, 田中 温, 竹本 洋一: "赤外分光分析によるX, Y精子の判別に関する研究"第61回農業機械学会年次大会講演要旨. 437-438 (2002)

Ryo Koyama、Kenichi Kudo、Toshiro Higuchi、Takezumi Tanaka、Atsushi Tanaka、Yoichi Takemoto：“红外光谱法辨别 X 和 Y 精子的研究”日本农业机械工程师学会第 61 届年会摘要 437-438（。 2002）

TSUKADA, KUDOH, BUDIMAN, YAMAMOTO, HIGUCHI, KOBAYASHI, SATO: "Rotational Position Control Biological Cell by Electrostatic Force"SICE Annual Conference 2002. 1265-1269 (2002)

Tsukada, KUDOH, BUDIMAN, YAMAMOTO, Higuchi, KOBAYASHI, SATO：“通过静电力控制生物细胞的旋转位置”SICE 年会 2002. 1265-1269 (2002)

工藤 謙一, ブディマン ミシェル, 塚田 修大, 樋口 俊郎, 吉田光敏: "電磁衝撃力を用いた細胞操作用マイクロインジェクションシステムの開発"第15回「電磁力関連のダイナミクス」シンポジウム. (印刷中). (2003)

Kenichi Kudo、Michel Budiman、Osamu Tsukada、Toshiro Higuchi、Mitsutoshi Yoshida：“利用电磁冲击力进行细胞操作的显微注射系统的开发”第 15 届“与电磁力相关的动力学”研讨会（2003 年出版）。

KUDOH, BUDIMAN, TSUKADA, HIGUCHI, YOSHIDA: "Development of Micro Injection System for Cell Manipulation Using Piezoelectric Impact"The 15th symposium on Electromagnetics and Dynamics. printing. (2001)

KUDOH、BUDIMAN、TSUKADA、HIGUCHI、YOSHIDA：“利用压电冲击进行细胞操纵的微注射系统的开发”第 15 届电磁学和动力学研讨会。

Michel Budiman, 工藤謙一, 樋口俊郎: "圧電素子と電磁力を組み合わせた駆動機構を用いたマイクロマニピュレータの開発"第13回「電磁力関連のダイナッミクス」シンポジウム講演論文集. No.01-251. 767-770 (2001)

Michel Budiman、Kenichi Kudo、Toshiro Higuchi：“使用压电元件和电磁力相结合的驱动机构的微操纵器的开发”第 13 届“与电磁力相关的动力学”研讨会论文集第 767-770 号。 ）