Development of microinjection system

显微注射系统的开发

• 批准号: 10555084
• 负责人: KUDOH Ken-ichi
• 金额: $ 7.94万
• 依托单位: The University of Tokyo (1999-2000)Kanagawa Academy of Science and Technology (1998)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10555084/
• 关键词: Micromanipulator; Microinjection; Micromanipulation; Image processing; 圧電素子

We developed element technologies that are necessary for the automation of microinjection system that intended for handling DNA, a nucleus, sperm etc. And by combining them with the micropipette drive system using piezoelectric impact drive mechanism and installing them to a microscope, we developed a high efficiency automated microinjection system for micro insemination.The developed element technologies for microinjection system are these following three subjects.(1) Cell Sorter MechanismWe developed a Cell Sorter Mechanism for gathering egg cells from any position in the nutrient medium to the center of microscope view by using wave force, which occurred by shaking dish container that filled with egg cells.(2) Cell Rotation MechanismIn enucleating operation, to control the position of an egg which considered the position of the nucleus and the polar body is known as a difficult operation and requiring high skill of the operator. Hence, we developed cell rotation mechanism using elec … More trostatic force to rotate and control the position of the egg.(3) Cell Positioning System Using Image Process TechnologyIn case of dealing with a small amount of cells, we developed a system for positioning an egg cell in the center of work area by using image processing technology.We combined the mechanism mentioned above with the micropipette drive system using piezoelectric impact drive mechanism and made the prototype of the automated microinjection system, which installed to an inverted microscope. In addition, we also did fundamental researches and experiments of cell rotation mechanism using electrostatic force and in the experiments using mouse egg cells, the development rate after the fertilization was confirmed.In the research period, we did movement test, evaluation and examination on the prototype we developed in Suruga Seiki Co., Ltd. The prototype is also used for real cell operation in Nihon University College of Bioresource Sciences, Tokai University School of Medicine, and Kagoshima University Faculty of Agriculture, and got many advices and knowledge. Less

我们开发了用于处理DNA、细胞核、精子等的微注射系统自动化所必需的元件技术。通过将它们与采用压电式冲击驱动机构的微吸管驱动系统相结合，并将它们安装在显微镜上，我们开发了一种用于显微受精的高效自动微注射系统。所开发的微注射系统的元件技术包括以下三个主题：(1)细胞分选机构我们开发了一种细胞分选机构，通过摇动装满卵细胞的培养皿中的波浪力，将卵细胞从营养介质中的任何位置收集到显微镜的中心。(2)细胞旋转机构在去核手术中，控制鸡蛋的位置，考虑到核和极体的位置，是一项困难的操作，对操作员的技能要求很高。因此，我们开发了使用ELEC…的细胞旋转机制基于图像处理技术的细胞定位系统在处理少量细胞的情况下，利用图像处理技术开发了一种将鸡蛋细胞定位在工作区中央的系统，并将上述机构与采用压电式冲击驱动机构的微吸管驱动系统相结合，制作了安装在倒置显微镜上的自动微量注射系统的原型。此外，我们还利用静电力对细胞旋转机制进行了基础研究和实验，在小鼠卵细胞实验中，确定了受精后的发育率。在研究期间，我们对Suruga Seiki株式会社开发的样机进行了运动测试、评估和检验。该样机也被用于日本大学生物资源学院、东海大学医科大学和鹿儿岛大学农学院的真实细胞操作，得到了许多建议和知识。较少

项目成果

塚田,工藤,山本,鳥居,樋口,小林,佐藤: "卵細胞回転機構の開発"Journal of Mammalian Ova Reserch. (2000)

Tsukada、Kudo、Yamamoto、Torii、Higuchi、Kobayashi、Sato：“卵细胞旋转机制的发展”哺乳动物卵子研究杂志（2000）。

KUDOH, TABUCHI, HIGUCHI: "Development of cell line mechanism using the flow of liquid"The 4th Intelligence Mechatoronics work shop. 36-40 (1999)

KUDOH、TABUCHI、HIGUCHI：“利用液体流动开发细胞系机制”第四届智能机电学研讨会。

KUDOH, TABUCHI, HIGUCHI: "Development of cell line mechanism using the flow of liquid"Journal of japan society for precision engineering. 301, spring. (1999)

KUDOH、TABUCHI、HIGUCHI：“利用液体流动开发细胞系机制”日本精密工程学会杂志。

工藤,塚田,山本,樋口,大石,飯田: "マイクロマニピュレーション操作の自動制御とその応用"第1回日本医用ミニブタ研究会大会シンポジウム抄録集. 1-1 (2000)

Kudo、Tsukada、Yamamoto、Higuchi、Oishi、Iida：“显微操作操作的自动控制及其应用”第一届日本医学小型猪研究会研讨会摘要 1-1（2000 年）。

塚田,工藤,山本,樋口: "細胞操作用マイクロマニピュレータの自動化に関する研究"第59回農業機械学会年次大会講演要旨集. (2000)

Tsukada、Kudo、Yamamoto、Higuchi：“用于细胞操作的显微操作器的自动化研究”日本农业机械学会第 59 届年会摘要（2000 年）。