Development of cell line mechanism

细胞系机制的开发

• 批准号: 10650268
• 负责人: KUDOH Ken-ichi
• 金额: $ 2.18万
• 依托单位: The University of Tokyo (1999)Kanagawa Academy of Science and Technology (1998)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10650268/
• 关键词: Micromanipulator; Microinjection; Micromanipulation; Image processing; 細胞整列; 精密加工; ボイスコイルモータ

A square case containing ova and culture medium was vibrated using a voice coil motor on purpose to gather those ova at the center of the mentioned case. Followed by, only those ova which conform to the memorized shape are selected with a picture processing device and are positioned at the point of holding pipette one by one. A system with mentioned functions was constructed on trial.This system can position automatically any ovum at the fixed position, and therefore could shorten the time of the process to search out the ovum and position it at the point of holding pipette.Prepositioning partThe device which shakes the Petri dish and square case filled with medium by using a voice coil motor was made for trial purposes. The voice coil motor is controlled by analog PID controller which compares the voltage from the transmitter with the displacement voltage detected by the eddy current gap sensor. The small square case is fixed in a large square case installed at the axis of the voice coil motor. A voltage wave is inputted by the transmitter to control the voice coil motor, and the square case is shaken. This vibration is used to gather the ova.

一个装有卵子和培养液的方形盒子被故意用音圈马达振动，以将这些卵子聚集在所述盒子的中心。然后，用图像处理装置选择符合记忆形状的卵子，并逐个放置在吸管夹持点。在试验中构建了一个具有上述功能的系统，该系统可以自动将任何卵子定位在固定位置，从而缩短了找出卵子并将其定位在固定吸管的过程中的时间。音圈电机由模拟PID控制器控制，将变送器输出的电压与电涡流间隙传感器检测到的位移电压进行比较。小方壳固定在安装在音圈电机轴线上的大方壳内。变送器输入电压波控制音圈电机，震动方壳。这种振动被用来收集卵子。

项目成果

KUDOH, TABUCHI, HIGUCHI: "Development of cell line mechanism using the flow of liquid"Journal of japan society for precision engineering. 301, spring. (1999)

KUDOH、TABUCHI、HIGUCHI：“利用液体流动开发细胞系机制”日本精密工程学会杂志。

KUOOH, TABUCHI, HIGUCHI: "Development of cell line mechanism using the flow of liquid"The 4th Intelligence Mechatoronics work shop. 36-40 (1999)

KUOOH、TABUUCHI、HIGUCHI：“利用液体流动开发细胞系机制”第四届智能机电学研讨会。

塚田,工藤,山本,鳥居,樋口,小林,佐藤: "卵細胞回転機構の開発"Journal of Mammalian Ova Reserch. (2000)

Tsukada、Kudo、Yamamoto、Torii、Higuchi、Kobayashi、Sato：“卵细胞旋转机制的发展”哺乳动物卵子研究杂志（2000）。

KUDOH, TABUCHI, HIGUCHI, KAKUSYO, SATO: "Development of automatic micromanipulation system for biological cell sorter"Journal of Mammalian Ova Research. Vol. 15. 167-172 (1998)

KUDOH、TABUCHI、HIGUCHI、KAKUSYO、SATO：“生物细胞分选仪自动显微操作系统的开发”哺乳动物卵子研究杂志。

田渕,工藤,樋口,佐藤,楠元: "加振による波流を利用した細胞整列機構" 1999年度精密工学会春季大会学術講演会講演論文集. (1999)

Tabuchi、Kudo、Higuchi、Sato、Kusumoto：“利用振动引起的波流的单元对准机制”1999 年日本精密工程学会春季学术会议论文集（1999 年）。