Development of a cryogenic temperature cell operating system

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The equipment which makes a hole in the egg cell frozen using very low temperature environment was developed. And the equipment made injection of the sperm possible.The equipment which makes a hole in the egg cell frozen using very low temperature environment was developed. And the equipment made injection of the sperm possible. Roe and a mouse ovum were used in the experiment. Each cell, it froze and a hole is made by Machining by a drill. By freezing, a soft cell become hard and processing by the drill or an Endmill is attained. It succeed in making a hole(50μand 30μ) each cell. Sperm injection was tried with the mouse egg. However, fetilzation was not able to be checked.