Optical Resolution with Molecularly Imprinted Membranes Having Nanometer Sized Chiral Recognition Sites

具有纳米尺寸手性识别位点的分子印迹膜的光学分辨率

• 批准号: 12450317
• 负责人: YOSHIKAWA Masakazu
• 金额: $ 3.78万
• 依托单位: Kyoto Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12450317/
• 关键词: Molecular Recognition; Chiral Recognition; Optical Resolution; Molecular Imprinting; Nanometer-Sized Materials; Peptides; Membranes; Print Molecules; 分子確認; 不斉確認

The following results have been obtained through the present research project: The chiral recognition ability for eight types of tripeptides, consisiting of L-glutamic acid γ-benzyl ester and L-phenylalanine was studied. The chiral recognition ability depends on both the tripeptide sequence and the amino acid residue content. Tripeptide derivatives containing more glutamic acid derivative residue or glutamic acid derivative as an amino-terminal residue show higher molecular recognition ability.The chiral recognition ability of molecularly imprinted tetrapeptide derivatives, consisting of L-aspartic acid cyclohexyl ester or L-glutamic acid γ-benzyl ester was also studied.The effect of the polarity of the environment of the molecular recognition ability for molecularly imprinted tetrapeptide derivatives was also investigated. The optimum composition, with best chiral recognition, is a 50 vol% aqueous ethanol solution.Molecularly imprinted polymeric membranes were prepared from various oligopeptide tweezers. They recognized the D-isomer, of which absolute configuration was the same as that of the print molecule, from racemic Ac-Trp mixtures, while the oligopeptide tweezers consisted of L-amino acid residues.The imprinted membranes from polyamide AMODEL showed highe adsorption selectivity and permselectivity toward bisphenol A than the control membrane.

通过本研究项目获得了以下结果：八种类型三肽的手性识别能力，l-谷氨酸γ-苯唑酯的合成和L-苯基丙氨酸是研究的。手性识别能力取决于三肽序列和氨基酸保留含量。三肽衍生物含有更多的谷氨酸衍生物或谷氨酸衍生物作为氨基末端居住物，表现出较高的分子识别能力。分子识别能力的分子识别能力，由L-间谍酸环己基酯或l-甲基酸酸酯的甲基酸酯的l-甲基甲基酯类的l-甲基甲基苯甲酸酯的酯类酸酯类的酯类酸酯的酯类酸酯的酯类酸酯类的神经甲基酯的酯类酸酯类的酯类酸酯的效应。还研究了分子不张纸静脉穿刺剂衍生物的分子识别能力。具有最佳手性识别的最佳成分是50 vol％水性乙醇溶液。从各种寡肽镊子中制备了分子上的印迹聚合物膜。他们认识到D-ISOMER，其绝对构型与印刷分子相同，来自Racemic AC-TRP混合物，而寡肽镊子由L-氨基酸保留组成，来自聚酰胺Amodel的磁性膜显示出高吸附选择性和允许对Bisphenol的允许性偏见，而不是对控制型的频率。