Development of Alternative Molecularly lmprinted Membranes

替代分子印迹膜的开发

• 批准号: 10650760
• 负责人: YOSHIKAWA Masakazu
• 金额: $ 2.11万
• 依托单位: KYOTO INSTITUTE OF TECHNOLOGY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10650760/
• 关键词: Chiral Recognition; Molecular Recognition; Optical Resolution; Molecular lmprinting; Amino Acids; Peptides; Membrane Separation; Permselectivity

It is not only an interesting but also an unsolved subject to develop artificial receptors. The molecular imprinting technique is a facile method to impart molecular recognition sites in synthetic polymers. In the present study, eight kinds of tripeptide derivatives, which consists of Glu (OBzl) and Phe, were adopted as candidate materials to form chiral recognition sites.The membranes imprinted by L-isomer recognize L-isomer in preference to corresponding D-isomer.To study the substrate specificity of the recognition site, the adsorption isotherms were investigated. The adsorption isotherm for D-isomer is a straight line passing through the origin, implying that the D-isomer is adsorbed by the membrane without any specific interaction. On the other hand, that of L-isomer has a complicated profile, implying that the adsorption consists of non-specific adsorption and adsorption on a specific recognition site for the L-isomer.By adopting optimum potential difference, electrodialysis of the racemic amino acid showed that permselectivity directly reflects its adsorption selectivity.

这不仅是一个有趣的人造受体的有趣的主题。分子烙印技术是一种在合成聚合物中赋予分子识别位点的便捷方法。在本研究中，由GLU（OBZL）和PHE组成的八种三种三肽衍生物被用作形成手性识别位点的候选材料。l-Isomer识别l-Isomer识别l-Isomer识别对相应的d-isomer的识别的膜。用于研究相应的D-ISOMER。研究识别位置的识别特异性，研究了ADSORSORMETTINT，ADSORSORSOSSMOSSSMOSSSMOSSOSSMOSTORSESSORTHESSSOSSORTHESS sOSSORTHENTS。 D-Isomer的吸附等温线是通过原点的直线，这意味着D-Isomer被膜吸附而没有任何特定相互作用。另一方面，L-异构体具有复杂的特征，这意味着吸附是由非特异性的吸附和吸附在L-ISomer的特定识别位点上的吸附。通过采用最佳电位差异，种族氨基酸的电溶解度可以直接反映其吸附性的允许性。

项目成果

M. Yoshikawa et al.: "Molecularly imprinted polymeric membranes having EFF derivative as a chiral recognition site"Macromal,. Chem. Phys.. 200. 1458-1465 (1999)

M. Yoshikawa 等人：“具有 EFF 衍生物作为手性识别位点的分子印迹聚合物膜”Macromal，。

M.Yoshikawa et al.: "Molecularly imprinted polymeric membranes having EFF derivatives as a chiral recognition site"Macromol.Chem.Phys.. 200. 1458-1465 (1999)

M.Yoshikawa 等：“具有 EFF 衍生物作为手性识别位点的分子印迹聚合物膜”Macromol.Chem.Phys.. 200. 1458-1465 (1999)

M. Yoshikawa et al.: "Molecularly imprinted polymeric membranes having EFF derivative as a chiral recognition site"macromol. Chem. Phys.. 200. 1458-1465 (1999)

M. Yoshikawa 等人：“具有 EFF 衍生物作为手性识别位点的分子印迹聚合物膜”macromol。