Development of Alternative Molecularly lmprinted Membranes

替代分子印迹膜的开发

• 批准号: 10650760
• 负责人: YOSHIKAWA Masakazu
• 金额: $ 2.11万
• 依托单位: KYOTO INSTITUTE OF TECHNOLOGY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10650760/
• 关键词: Chiral Recognition; Molecular Recognition; Optical Resolution; Molecular lmprinting; Amino Acids; Peptides; Membrane Separation; Permselectivity

It is not only an interesting but also an unsolved subject to develop artificial receptors. The molecular imprinting technique is a facile method to impart molecular recognition sites in synthetic polymers. In the present study, eight kinds of tripeptide derivatives, which consists of Glu (OBzl) and Phe, were adopted as candidate materials to form chiral recognition sites.The membranes imprinted by L-isomer recognize L-isomer in preference to corresponding D-isomer.To study the substrate specificity of the recognition site, the adsorption isotherms were investigated. The adsorption isotherm for D-isomer is a straight line passing through the origin, implying that the D-isomer is adsorbed by the membrane without any specific interaction. On the other hand, that of L-isomer has a complicated profile, implying that the adsorption consists of non-specific adsorption and adsorption on a specific recognition site for the L-isomer.By adopting optimum potential difference, electrodialysis of the racemic amino acid showed that permselectivity directly reflects its adsorption selectivity.

人工受体的开发是一个有趣的问题，也是一个尚未解决的问题。分子印迹技术是一种在合成聚合物中赋予分子识别位点的简便方法。本研究采用Glu （OBzl）和Phe组成的8种三肽衍生物作为候选材料形成手性识别位点。l -异构体印迹膜优先识别l -异构体而不是相应的d -异构体。为了研究识别位点的底物特异性，研究了吸附等温线。d -异构体的吸附等温线是一条穿过原点的直线，这意味着d -异构体被膜吸附，没有任何特定的相互作用。另一方面，l -异构体的吸附具有复杂的轮廓，这意味着吸附分为非特异性吸附和在l -异构体的特定识别位点上的吸附。采用最优电位差对外消旋氨基酸进行电渗析，结果表明其过选择性直接反映了其吸附选择性。

项目成果

M. Yoshikawa et al.: "Molecularly imprinted polymeric membranes having EFF derivative as a chiral recognition site"Macromal,. Chem. Phys.. 200. 1458-1465 (1999)

M. Yoshikawa 等人：“具有 EFF 衍生物作为手性识别位点的分子印迹聚合物膜”Macromal，。

M.Yoshikawa et al.: "Molecularly imprinted polymeric membranes having EFF derivatives as a chiral recognition site"Macromol.Chem.Phys.. 200. 1458-1465 (1999)

M.Yoshikawa 等：“具有 EFF 衍生物作为手性识别位点的分子印迹聚合物膜”Macromol.Chem.Phys.. 200. 1458-1465 (1999)

M. Yoshikawa et al.: "Molecularly imprinted polymeric membranes having EFF derivative as a chiral recognition site"macromol. Chem. Phys.. 200. 1458-1465 (1999)

M. Yoshikawa 等人：“具有 EFF 衍生物作为手性识别位点的分子印迹聚合物膜”macromol。