DEVELOPMENT OF SYNTHETIC LIPOSOMES WITH CERAMIC SURFACE AND THEIR NOVEL FUNCTIONS

陶瓷表面合成脂质体的开发及其新功能

• 批准号: 12450364
• 负责人: KIKUCHI Jun-ichi
• 金额: $ 9.28万
• 依托单位: NARA INSTITUTE OF SCIENCE AND TECHNOLOGY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12450364/
• 关键词: ORGANIC-INORGANIC HYBRID; CERASOME; NANO-PARTICLE; CERAMICS; LIPOSOME; BILAYER MEMBRANE; ARTIFICIAL MULTICELLULAR SYSTEM; ARTIFICIAL ORGAN; 光触媒; 分子デバイス; 交互積層; 多細胞モデル; 光触媒機能; ゾル-ゲル法; 脂質リポソーム; 膜物性

The aim of this research project is to develop liposomal vesicies having ceramic surfaces as novel organic-inorganic hybrid nanomaterials. Liposomes, organic bilayer vesicles formed with lipids such as biomembrane-forming phospholipids, have been employed as biomembrane models and drug carriers. On the other hand, in recent years, much attention has been focused on unique functions of organic-inorganic hybrids which were derived from fusion of characteristics of organic materials with those of inorganic ones. On these grounds, we designed and prepared a novel organic-inorganic hybrid vesicle, which is a liposome coated with silica ceramics, and investigated in their novel functions. Topics in this research are as follows.(1) Development of organic-inorganic hybrid vesicles "Cerasomes"Liposome-forming double-chain lipids each having a trialkoxysilyl head moiety were designed and synthesized. In aqueous media, liposomes with inorganic frameworks like a silica ceramic on the surface were … More successfully prepared through hydrolysis of the head moiety and followed condensation of the silanol head, and self-assembling under appropriate conditions. We named the hybrid vesicle "Cerasome".(2) Physical properties, surface modification, and functionalization of CarecomesMorphological stability of the Cearsomes was drastically increased as compared with the conventional liposomes, while the Cerasomes possessed basic physical properties characteristic to the liposomes, such as compartmentation ability and gel-to-liquid-crystallibne phase transition behavior. Functionalization of the Cerasomes was achieved by surface modification of the nanohybrids.(3) Three-dimensional organization of CerasomesRemarkable morphological stability of the Cerasomes led us to construct hierarchical and three-dimensional architectures of the liposomal vesicles on substrate by employing layer-by-layer assembling technique. Thus, the results of the present study would provide us a new promising research field of the liposome chemistry for aiming to develop artificial multicellular systems and artificial organs. Less

本研究的目的是开发具有陶瓷表面的脂质体囊泡作为新型有机-无机杂化纳米材料。脂质体是由磷脂等脂质形成的有机双层囊泡，已被用作生物膜模型和药物载体。另一方面，近年来，有机-无机杂化材料的独特功能受到了广泛关注，这些功能是由有机材料的特性与无机材料的特性融合而产生的。在此基础上，我们设计并制备了一种新型的有机-无机杂化囊泡，即包覆有二氧化硅陶瓷的脂质体，并对其新功能进行了研究。本研究的主题如下。(1)有机-无机杂化囊泡“Cerasomes“的开发设计并合成了每个具有三烷氧基甲硅烷基头部部分的脂质体形成双链脂质。在水性介质中，脂质体与无机框架，如二氧化硅陶瓷的表面上， ...更多信息 通过水解头部部分，然后缩合硅烷醇头部，并在适当条件下自组装成功制备。我们将这种混合囊泡命名为“Cerasome”。(2)Caresomes的物理性质、表面修饰和功能化与常规脂质体相比，Cerasomes的形态稳定性显著提高，而Cerasomes具有脂质体的基本物理性质，如隔室化能力和凝胶-液体-结晶相变行为。神经酰胺脂质体的功能化通过纳米杂化物的表面改性实现。(3)Cerasomes的三维结构Cerasomes的显著形态稳定性使我们能够通过层层组装技术在基底上构建分层和三维结构的脂质体囊泡。因此，本研究的结果将为我们提供一个新的有前途的研究领域的脂质体化学旨在开发人工多细胞系统和人工器官。少

项目成果

有賀克彦: "有機・無機ハイブリッドベシクル"Cerasome"の作製条件"高分子論文集. 57・4. 251-253 (2000)

有贺克彦：“有机-无机混合囊泡“Cerasome”的生产条件”《聚合物研究杂志》57・4（2000）。