DEVELOPMENT OF SYNTHETIC LIPOSOMES WITH CERAMIC SURFACE AND THEIR NOVEL FUNCTIONS

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The aim of this research project is to develop liposomal vesicies having ceramic surfaces as novel organic-inorganic hybrid nanomaterials. Liposomes, organic bilayer vesicles formed with lipids such as biomembrane-forming phospholipids, have been employed as biomembrane models and drug carriers. On the other hand, in recent years, much attention has been focused on unique functions of organic-inorganic hybrids which were derived from fusion of characteristics of organic materials with those of inorganic ones. On these grounds, we designed and prepared a novel organic-inorganic hybrid vesicle, which is a liposome coated with silica ceramics, and investigated in their novel functions. Topics in this research are as follows.(1) Development of organic-inorganic hybrid vesicles "Cerasomes"Liposome-forming double-chain lipids each having a trialkoxysilyl head moiety were designed and synthesized. In aqueous media, liposomes with inorganic frameworks like a silica ceramic on the surface were … More successfully prepared through hydrolysis of the head moiety and followed condensation of the silanol head, and self-assembling under appropriate conditions. We named the hybrid vesicle "Cerasome".(2) Physical properties, surface modification, and functionalization of CarecomesMorphological stability of the Cearsomes was drastically increased as compared with the conventional liposomes, while the Cerasomes possessed basic physical properties characteristic to the liposomes, such as compartmentation ability and gel-to-liquid-crystallibne phase transition behavior. Functionalization of the Cerasomes was achieved by surface modification of the nanohybrids.(3) Three-dimensional organization of CerasomesRemarkable morphological stability of the Cerasomes led us to construct hierarchical and three-dimensional architectures of the liposomal vesicles on substrate by employing layer-by-layer assembling technique. Thus, the results of the present study would provide us a new promising research field of the liposome chemistry for aiming to develop artificial multicellular systems and artificial organs. Less