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Preparation and Functionalization of Artificial Multi-cellular Systems by employing Organic-Inorganic Hybrid Nanocapsules

有机-无机杂化纳米胶囊人工多细胞系统的制备和功能化

基本信息

批准号：
15205022
负责人：
KIKUCHI Jun-ichi
金额：
$ 32.86万
依托单位：
Nara Institute of Science and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

Recently, we have developed Cerasome, a novel artificial cell membrane with high morphological stability. In the present study, we constructed artificial multi-cellular systems by employing such stable membranes and evaluated their functions as molecular devices. Topics obtained in this research are follows.1.In order to establish the guidepost for molecular design of the lipids which are able to control the aggregate morphology of the Cerasome, various types of the Cerasome-forming lipids were prepared. Physicochemical characterization of the lipid aggregates were performed by means of transmission and scanning electron microscopy, atomic force microscopy, differential scanning calorimetry, and dynamic light scattering measurements. As a result, we established the preparation procedures of various Cerasomes with a size in a range of 50-5000 nm.2.Intra- and inter-membrane interactions to create highly organized architectures of the artificial cell membranes were evaluated. For hierarch … More ical assembly of the artificial cells, multipoint and multi-functional assistance of intermolecular interactions, such as electrostatic and metal-coordination interactions, were found to be much effective.3.Functional factors to develop the molecular devices using the artificial celluls were clarified. For example, we observed effective intermolecular communication between an artificial receptor and a natural enzyme on a platform of the artificial membranes such as the Cerasome. We also constructed an artificial multi-cellular system in which reversible assembly of the cells was controlled by logic response of a molecular switch embedded in the membrane. Highly efficient charge separation was observed in an artificial cell containing fullerenes. Appropriate external signals such as biomolecules, ions, photons, and thermal stimuli effectively control the functions of artificial cells as the molecular devices.In conclusion, the artificial cells designed by introducing the concept of the organic-inorganic hybrids are potent self-organized materials in the research area of bio-nano science. Less

最近，我们开发了Cerasome，一种具有高形态稳定性的新型人工细胞膜。在本研究中，我们构建了人工多细胞系统，采用这种稳定的膜，并评估其功能的分子器件。本研究的主要内容如下：1.制备了不同类型的形成Cerasome的脂质，为控制Cerasome聚集形态的脂质分子设计提供了指导。通过透射和扫描电子显微镜、原子力显微镜、差示扫描量热法和动态光散射测量对脂质聚集体进行理化表征。因此，我们建立了各种神经酰胺脂质体的制备程序，其尺寸在50-5000 nm范围内。2.评价了膜内和膜间相互作用，以创建高度有序的人工细胞膜结构。为教主 ...更多信息人工细胞的组装、分子间相互作用的多点和多功能辅助（如静电作用和金属配位作用）是非常有效的。3.阐明了利用人工细胞研制分子器件的功能因素。例如，我们观察到人工受体和天然酶之间的有效分子间通信的人工膜的平台，如Cerasome。我们还构建了一个人工多细胞系统，其中细胞的可逆组装由嵌入在膜中的分子开关的逻辑响应控制。在含有富勒烯的人工电池中观察到高效的电荷分离。合适的外界信号如生物分子、离子、光子、热刺激等可以有效地控制人工细胞作为分子器件的功能，引入有机-无机杂化物的概念设计的人工细胞是生物纳米科学研究领域中一种很有潜力的自组织材料。少