STUDIES ON CONTROL OF ENZYME REANTIONS PERFORMED BY SUPRAMOLECULAR NANO-REACTORS

超分子纳米反应器酶反应控制研究

• 批准号: 09450332
• 负责人: KIKUCHI Jun-ichi
• 金额: $ 3.2万
• 依托单位: NARA INSTITUTE OF SCIENCE AND TECHNOLOGY (1998)Kyushu University (1997)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09450332/
• 关键词: Supramolecular Assembly; Nano-reactor; Enzyme Reaction; Artificial Receptor; Lipid Bilayer Vesicle; Molecular Recognition; Steroid Cyclophane; phase Reorganization; 人工レセプター; 合成脂質リポソーム

1. Supramolecular assemblies as nano-reactors being capable of controlling enzymic activity were constituted in combination with an artificial receptor, an enzyme, and a bilayer-forming lipid.2. Synthetic peptide lipids having an amino acid residue interposed between a polar head moiety and a hydrophobic double-chain segment formed morphologically stable bilayer vesicles as nano-scale reaction vessels.3. NADH-dependent lactate dehydrogenase was bound to the vesicular surface, mainly through electrostatic interactions, keeping a conformation of the active site in aqueous media.4. The steroid cyclophane composed of three functional components, a cyclophane ring, four bile acid moieties, and four lysine residues connecting them, was prepared as an artificial receptor. When the receptor was embedded in the bilayer membrane, this molecule behaved as a molecular switch of the nano-reactor by performing double signal recognition.5. A bile acid derivative having an amino group acted as an artificial cell-surface receptor capable of activating lactate dehydrogenase by performing double signal recognition and phase reorganization in a synthetic bilayer membrane. Analogous behavior was also observed for an artificial transmembrane receptor having an azobenzene moiety.6. Aromatic aldehydes such as 1 -hydroxy-2-naphthaldehyde and cupper(II) ions were effective external and second messenger signals, respectively, for these artificial receptors.

1. 以人工受体、酶和双层成膜脂为载体，构建了具有控制酶活性的超分子组件纳米反应器。在极性头段和疏水双链段之间插入氨基酸残基的合成肽脂形成了形态稳定的双层囊泡作为纳米级反应容器。nadh依赖性乳酸脱氢酶主要通过静电相互作用与囊泡表面结合，在水介质中保持活性位点的构象。合成了由一个环环、四个胆汁酸基团和连接它们的四个赖氨酸残基组成的甾体环烷作为人工受体。当受体嵌入双层膜时，该分子通过双信号识别充当纳米反应器的分子开关。一种具有氨基的胆汁酸衍生物，作为人工细胞表面受体，能够通过在合成双层膜中进行双重信号识别和相重组来激活乳酸脱氢酶。具有偶氮苯基团的人工跨膜受体也观察到类似的行为。芳香醛如1 -羟基-2-萘醛和铜离子分别是这些人工受体有效的外部和第二信使信号。

项目成果

J.Kikuchi: "Steroid Cyclophanes as Artificial Cell-surface Receptors. Molecular Recognition and Its Consequence in Signal Transduction Behavior" J.Incl.Phenom.32巻2/3号. 209-221 (1998)

J.Kikuchi：“类固醇环烷作为人工细胞表面受体。分子识别及其在信号转导行为中的后果”J.Incl.Phenom.第 32 卷，第 209-221 期（1998 年）。

J.Kikuchi: "Steroid Cyclophanes as Artificial Cell-surface Receptors. Molecular Recognition and Its Consequence in Signal Transduction Behavior" J.Incl.Phenom.32 (2/3). 209-221 (1998)

J.Kikuchi：“类固醇环芳烷作为人工细胞表面受体。分子识别及其在信号转导行为中的后果”J.Incl.Phenom.32 (2/3)。

J.Kikuchi: "Signal Transduction Mediated by Artificial Cell-surface Receptors. Activation of Lactate Dehydrogenase Triggered by Molecular Recognition and Phase Reorganization of Bile Acid Derivatives Embedded in a Synthetic Bilayer membrane" Chem.Commun.(

J.Kikuchi：“人工细胞表面受体介导的信号转导。分子识别引发的乳酸脱氢酶的激活和嵌入合成双层膜中的胆汁酸衍生物的相重组”Chem.Commun.（

J.Kikuchi: Molecular Recognition and Inclusion. Kluwer Academic Publishers, 6 (1998)

J.Kikuchi：分子识别和包容。

Y.Hisaeda: "Preparation of Artificial Glycolipids and Their Aggregation Behavior in Aqueous Media" Tetrahedron Lett.38巻38号. 6713-6716 (1997)

Y. Hisaeda：“人工糖脂的制备及其在水介质中的聚集行为”，《Tetrahedron Lett》，第 38 卷，第 6713-6716 期（1997 年）