Liquid-Chromatography-Tandem Mass Spectrometer (LC-MS/MS)

该液相色谱串联质谱仪将作为基尔基督教阿尔布雷希茨大学新昆克研究大楼石勒苏益格-荷尔斯坦大学医院儿科I系儿科内分泌科大学分部的中央研究基础设施。其目的是取代技术上过时的以前的质谱计，它在科学上不再具有竞争力(降低了灵敏度和特异度)，并遭受着越来越频繁的缺陷。由于该公司有限的支持，目前的旧设备存在最终失败的重大威胁。新设备主要计划测量不同生物材料(类固醇图谱、类固醇代谢组学)中的类固醇激素及其前体和代谢物。它服务于地方、国家和国际儿科和儿科内分泌研究合作--例如，作为项目中的参考核心设施--在关于罕见类固醇合成障碍、性发育障碍和肥胖症的基础科学和临床研究方面。除了生化重点外，在分子内分泌学项目中，细胞培养和途径的表征还需要准确的类固醇测定。

The liquid chromatography tandem mass spectrometer shall serve as a central research infrastructure of the University division of Pediatric Endocrinology of the Department of Pediatrics I in the University Hospital of Schleswig-Holstein in the new Quincke research building of the Christian Albrechts University of Kiel. The aim is to replace the technically outdated previous mass spectrometer which is not scientifically competitive anymore (reduced sensitivity and reduced specificity) and which suffers from increasingly frequent defects. There is a significant threat of final failure of the current old device due to limitted support by the company. The new device is mainly planned to measure steroid hormones, their precursors and metabolites in diverse biomaterials (steroid profiling, steroid metabolomics). This serves in local, national and international pediatric and pediatric endocrine research collaborations – e.g. as a reference core facility in projects – in basic science and clinical research on rare steroid synthesis disorders, disorders of sex development and obesity. In addition to the biochemical focus, accurate steroid determinations are needed for the characterization of cell cultures and pathways in projects of molecular endocrinology.