Liquid-Chromatography-Tandem Mass Spectrometer (LC-MS/MS)

液相色谱串联质谱仪应作为位于基尔Christian Albrechts大学新Quincke研究大楼的石勒苏益格-荷尔斯泰因大学医院儿科I部儿科内分泌学大学分部的中心研究基础设施。其目的是取代技术上过时的以前的质谱仪，该质谱仪不再具有科学竞争力（灵敏度降低和特异性降低），并且缺陷越来越频繁。由于公司的支持有限，当前旧器械存在最终失效的重大威胁。新设备主要计划测量类固醇激素，其前体和代谢物在不同的生物材料（类固醇分析，类固醇代谢组学）。该中心致力于地方、国家和国际儿科和儿科内分泌研究合作，例如作为项目中的参考核心设施，开展罕见类固醇合成障碍、性发育障碍和肥胖症的基础科学和临床研究。除了生物化学的重点，需要准确的类固醇测定的表征细胞培养和分子内分泌学项目的途径。

The liquid chromatography tandem mass spectrometer shall serve as a central research infrastructure of the University division of Pediatric Endocrinology of the Department of Pediatrics I in the University Hospital of Schleswig-Holstein in the new Quincke research building of the Christian Albrechts University of Kiel. The aim is to replace the technically outdated previous mass spectrometer which is not scientifically competitive anymore (reduced sensitivity and reduced specificity) and which suffers from increasingly frequent defects. There is a significant threat of final failure of the current old device due to limitted support by the company. The new device is mainly planned to measure steroid hormones, their precursors and metabolites in diverse biomaterials (steroid profiling, steroid metabolomics). This serves in local, national and international pediatric and pediatric endocrine research collaborations – e.g. as a reference core facility in projects – in basic science and clinical research on rare steroid synthesis disorders, disorders of sex development and obesity. In addition to the biochemical focus, accurate steroid determinations are needed for the characterization of cell cultures and pathways in projects of molecular endocrinology.