Liquid-Chromatography-Tandem Mass Spectrometer (LC-MS/MS)

液相色谱串联质谱计将作为石勒苏益格-荷尔斯泰因大学医院儿科一科儿科内分泌科的中心研究基础设施，位于基尔克里斯蒂安阿尔布雷希茨大学新Quincke研究大楼内。其目的是取代技术上落后的以前的质谱仪，这些质谱仪在科学上已不再具有竞争力（灵敏度和特异性降低），并且缺陷日益频繁。由于公司有限的支持，目前的旧设备存在最终失效的重大威胁。新设备主要用于测量不同生物材料中的类固醇激素，其前体和代谢物（类固醇分析，类固醇代谢组学）。它在地方、国家和国际儿科和儿科内分泌研究合作中发挥作用-例如作为项目的参考核心设施-在关于罕见类固醇合成障碍、性发育障碍和肥胖的基础科学和临床研究中。除了生物化学的重点外，在分子内分泌学的项目中，精确的类固醇测定对于细胞培养和途径的表征是需要的。

The liquid chromatography tandem mass spectrometer shall serve as a central research infrastructure of the University division of Pediatric Endocrinology of the Department of Pediatrics I in the University Hospital of Schleswig-Holstein in the new Quincke research building of the Christian Albrechts University of Kiel. The aim is to replace the technically outdated previous mass spectrometer which is not scientifically competitive anymore (reduced sensitivity and reduced specificity) and which suffers from increasingly frequent defects. There is a significant threat of final failure of the current old device due to limitted support by the company. The new device is mainly planned to measure steroid hormones, their precursors and metabolites in diverse biomaterials (steroid profiling, steroid metabolomics). This serves in local, national and international pediatric and pediatric endocrine research collaborations – e.g. as a reference core facility in projects – in basic science and clinical research on rare steroid synthesis disorders, disorders of sex development and obesity. In addition to the biochemical focus, accurate steroid determinations are needed for the characterization of cell cultures and pathways in projects of molecular endocrinology.