Liquid Chromatography High Resolution Tandem Mass Spectrometry for Proteomics, Metabolomics, Epigenetics, and Synthetic Oligonucleotides.

我们要求提供资金，用于购买高压液相色谱高分辨率串联质谱系统（HPLC-HR-MS/MS）。** 该仪器系统对于Denina Simmons博士在安大略理工大学（安大略理工学院）水生组学实验室的研究计划的各个方面都是必不可少的，该研究计划由加拿大研究主席计划（Tier II）和NSERC（发现补助金，发现加速器补充和启动补充）资助。 所要求的系统还将从根本上扩展Jean-Paul Desaulniers博士的既定研究计划，该计划由四个NSERC计划（发现补助金（DG），2个合作研究开发（CRD）赠款和I2 I补助金）资助。** HPLC-HR-MS/MS系统将用于对复杂生物血浆和组织样品中存在的大小生物分子（即寡核苷酸、蛋白质、代谢物和药物）进行大规模和高通量测序、测量和表征，这些生物分子在合成反应后的相对纯的溶液中，或在环境基质（如水和沉积物）中。没有其他可用的仪器适用于这种多样化和要求苛刻的分析阵列，并且具有类似能力的附近设施没有足够的时间来支持Simmons博士的研究计划所需的小时数，也没有提供Simmons博士和Desaulniers博士进行新生物分析方法开发所需的灵活性。因此，推迟购置这一设备将严重阻碍西蒙斯博士研究方案的启动和Desaulniers博士既定研究的扩大。西蒙斯博士的研究项目侧重于环境影响监测的非致命方法;她探讨了家庭污染物，采矿和工业废物，以及气候驱动的水生压力源，如有害藻华，缺氧和入侵物种对野生鱼类种群健康的影响。Desaulniers博士的研究计划涉及开发新型合成寡核苷酸，可用于治疗因基因过度表达而发生的疾病，并创造新的修饰，以改善递送并减少非靶效应。在未来5年内，所要求的设备将支持西蒙斯博士和德索尼尔博士联合研究小组至少30名高素质人员（包括本科生）的培训。所有HQP的培训将包括动手使用仪器，以及统计数据分析和大型和复杂的光谱数据集的解释，使用生物信息学和化学信息学软件。Simmons博士和Desaulniers博士都努力提供培训和研究机会，对所有人都具有包容性，并创建在性别，文化和社会多样性方面平衡的研究团队。

We request funding towards the purchase of a high-pressure liquid chromatography high-resolution tandem-mass spectrometry system (HPLC-HR-MS/MS). ******This instrument system is essential to all aspects of Dr. Denina Simmons' research program in the Aquatic Omics Lab at the University of Ontario Institute of Technology (Ontario Tech), which is funded by the Canada Research Chairs program (Tier II) and NSERC (Discovery grant, Discovery Accelerator Supplement, and a Launch Supplement). The requested system will also fundamentally expand the established research program of Dr. Jean-Paul Desaulniers which is funded by four NSERC programs (Discovery Grant (DG), 2 Collaborative Research Development (CRD) Grants, and an I2I grant). ******The HPLC-HR-MS/MS system will be used to enable large-scale and high-throughput sequencing, measurement, and characterization of both large and small biomolecules (i.e. oligonucleotides, proteins, metabolites, and drugs) that are present complex biological plasma and tissue samples, in relatively pure solutions after synthesis reactions, or in environmental matrices such as water and sediment. There is no other available instrumentation that is suitable for such a diverse and demanding array of analyses, and nearby facilities with similar capabilities do not have enough time available to support the number of hours required by Drs. Simmons' research program, nor to do provide the flexiblity required by both Dr. Simmons and Dr. Desaulniers to perform new bioanalytical method development. Thus, a delay in the acquisition of this equipment will cause a substantial impediment to the start-up of Dr. Simmons' research program and the expansion of Dr. Desaulniers' established research.******Dr. Simmons' research program focuses on non-lethal methods for environmental effect monitoring; she explores the impacts of household contaminants, mining and industrial waste, as well as climate-driven aquatic stressors like harmful algal blooms, hypoxia, and invasive species on the health of wild fish populations. Dr. Desaulniers' research program involves developing novel synthetic oligonucleotides that can be used as therapeutic treatments for diseases that occur due to the over expression of genes, and creating novel modifications that improve delivery and reduce non-target effects.******Over the next 5 years, the requested equipment will support the training of at least 30 highly qualified personnel (including undergraduate students) in the combined research groups of Dr. Simmons and Dr. Desaulniers. Training for all HQP will include hands-on use of the instrument, as well as the statistical data analysis and interpretation of large and complex spectral data-sets, using bioinformatics and chemoinformatics software. Both Dr. Simmons and Dr. Desaulniers make effort provide training and research opportunities and that are inclusive to all people and create research teams that are balanced in gender, cultural, and social diversity.*****