Systematic isolation of genes encoding candidate proteins for human melanoma antigens using serial analysis of gene expression (SAGE) and EST database

使用基因表达系列分析 (SAGE) 和 EST 数据库系统分离编码人类黑色素瘤抗原候选蛋白的基因

基本信息

  • 批准号:
    12470180
  • 负责人:
  • 金额:
    $ 9.22万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2001
  • 项目状态:
    已结题

项目摘要

To identify genes preferentially expressed in melanocyte / melanoma, we have applied two methods; comparison of cDNA profiles generated by serial analysis of gene expression (SAGE) and of EST databases from various tissues. AcDNA profile of highly pigmented melanoma cell line SKme123 was first evaluated using SAGE. 25,997 tags consisting of 10,382 transcripts were sequenced. This melanoma SAGE library was compared to SAGE databases from normal tissues including brain, colon and testis, brain and colon cancers, and also compared to the melanocyte cDNA library. Two tags possibly encoding new melanocyte specific genes with relatively high frequency were identified, and their specific expression was confirmed by RT-PCR and Northern blot analysis. Full length clones of these sequences were isolated from the SKme123 λ phage. One was a splicing variant of TRP2; the other was a novel gene with unknown function. In the next study, by comparing EST databases from various tissues using computer, 80 possibly melanocyte specific genes were selected, then, their expression was evaluated using RT-PCR. Seven sequences were found to express only in melanocytes or melanoma, and the isolation of the full length cDNA were attempted. M43 was a splicing variant of AMI, previously identified melanoma antigen recognized by T cells. M44 encoded a novel melanocyte specific molecule. M125 was a splicing variant of Melastatin 1, a possible tumor marker for melanoma. M40, M109 and M674 were found to be a3'-sequence of the novel melanocyte specific gene. M216 may also have a novel melanocyte specific gene at its 5'-upstream. Therefore, melanocyte / melanoma specific genes can be systematically isolated using SAGE and EST databases. These melanocyte specific genes may be useful for research on biology of melanocytes as well as development of diagnostic and therapeutic methods for disorder of pigmentation and melanoma.
为了确定优先表达的基因在黑色素细胞/黑色素瘤,我们采用了两种方法;比较基因表达(SAGE)和EST数据库从各种组织的系列分析产生的cDNA图谱。首先使用SAGE评估了高度色素性黑色素瘤细胞系SKme 123的AcDNA谱。对由10,382个转录物组成的25,997个标签进行测序。将该黑色素瘤SAGE文库与来自正常组织(包括脑、结肠和睾丸、脑和结肠癌)的SAGE数据库进行比较,并且还与黑色素细胞cDNA文库进行比较。通过RT-PCR和北方印迹分析,确定了两个可能编码新的黑素细胞特异性基因的标签,并证实了它们的特异性表达。从SKme 123 λ噬菌体分离这些序列的全长克隆。一个是TRP 2的剪接变体,另一个是功能未知的新基因。在接下来的研究中,通过计算机比较来自不同组织的EST数据库,选择了80个可能的黑素细胞特异性基因,然后使用RT-PCR评估它们的表达。发现7个序列仅在黑素细胞或黑素瘤中表达,并尝试分离全长cDNA。M43是AMI的剪接变体,AMI是先前鉴定的由T细胞识别的黑色素瘤抗原。M44编码一种新的黑素细胞特异性分子。M125是Melastatin 1的剪接变体,Melastatin 1可能是黑色素瘤的肿瘤标志物。M40、M109和M674为黑素细胞特异性新基因的3 '端序列。M216的5 ′上游可能还含有一个新的黑素细胞特异性基因。因此,黑素细胞/黑色素瘤特异性基因可以使用SAGE和EST数据库系统地分离。这些黑素细胞特异性基因可能对黑素细胞生物学的研究以及色素沉着和黑色素瘤的诊断和治疗方法的发展有帮助。

项目成果

期刊论文数量(51)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kuwana M, Kawakami, Y., et al.: "Induction of antigen-specific human CD4+ T cell anergy by peripheral blood DC2 precursors"Eur.J.Immunol.. 31・9. 2547-2557 (2001)
Kuwana M,Kawakami,Y.等人:“外周血DC2前体诱导抗原特异性人CD4+T细胞无反应性”Eur.J.Immunol.. 31·9(2001)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Kuwana M, Kawakami Y, et al.: "Induction of antigen-specific human CD4+ T cell anergy by peripheral blood DC2 precursors"Eur. J. Immunol. 31・9. 2547-2557 (2001)
Kuwana M、Kawakami Y 等:“外周血 DC2 前体诱导抗原特异性人 CD4+ T 细胞无反应性”Eur. J.Immunol. 2547-2557 (2001)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Kawakami Y. et al.: "T cell responses to melanoma and melanocytes."Pigment Cell Research. 13. 163-169 (2000)
Kawakami Y. 等人:“T 细胞对黑色素瘤和黑色素细胞的反应。”色素细胞研究。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Kuwana M. et al.: "Induction of antigen-specific human CD4+ T cell anergy by peripheral blood DC2 precursors"Eur. J. Immunol.. 31 (9). 2547-2557 (2001)
Kuwana M.等人:“外周血DC2前体诱导抗原特异性人CD4 T细胞无反应性”Eur。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

KAWAKAMI Yutaka其他文献

Immunosuppression caused by IDO protein stability in tumor microenvironment
IDO蛋白在肿瘤微环境中的稳定性引起的免疫抑制
  • DOI:
  • 发表时间:
    2019
  • 期刊:
  • 影响因子:
    0
  • 作者:
    TSUKAMOTO Nobuo;KUROSAKI Sou;INOUE Jun-ichiro;KAWAKAMI Yutaka
  • 通讯作者:
    KAWAKAMI Yutaka

KAWAKAMI Yutaka的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('KAWAKAMI Yutaka', 18)}}的其他基金

Investigation of differential immune status among cancer patients and development of personalized cancer therapy by combining immunomodulation
癌症患者差异免疫状态的研究及结合免疫调节的个体化癌症治疗的发展
  • 批准号:
    26221005
  • 财政年份:
    2014
  • 资助金额:
    $ 9.22万
  • 项目类别:
    Grant-in-Aid for Scientific Research (S)
Systematic analysis of cellular signaling involved in the melanoma induced immunosuppression
黑色素瘤诱导的免疫抑制中涉及的细胞信号传导的系统分析
  • 批准号:
    25670506
  • 财政年份:
    2013
  • 资助金额:
    $ 9.22万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Development of effective immunotherapy by combining interventions on key points in the anti-tumor immune network
结合抗肿瘤免疫网络关键点的干预开发有效的免疫疗法
  • 批准号:
    23240128
  • 财政年份:
    2011
  • 资助金额:
    $ 9.22万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Investigation of roles of miRNA in the immunosuppression and clinical use for patients with melanoma
miRNA在黑色素瘤患者免疫抑制中的作用及其临床应用研究
  • 批准号:
    23659554
  • 财政年份:
    2011
  • 资助金额:
    $ 9.22万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Development of effective immunotherapy by combining methods to restore immunocompetence of cancer patients
通过组合方法开发有效的免疫疗法以恢复癌症患者的免疫能力
  • 批准号:
    19390355
  • 财政年份:
    2007
  • 资助金额:
    $ 9.22万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of immunotherapy based on the identification of tumor antigens
基于肿瘤抗原鉴定的免疫疗法的发展
  • 批准号:
    17016070
  • 财政年份:
    2005
  • 资助金额:
    $ 9.22万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
Development of new diagnostic and immunotherapeutic methods for patients with cancer
为癌症患者开发新的诊断和免疫治疗方法
  • 批准号:
    14104013
  • 财政年份:
    2002
  • 资助金额:
    $ 9.22万
  • 项目类别:
    Grant-in-Aid for Scientific Research (S)
Development of diagnositic and therapeutic methods using frameshift mutated peptides for colorectal cancers with microsatellite instability
使用移码突变肽开发微卫星不稳定性结直肠癌的诊断和治疗方法
  • 批准号:
    12557109
  • 财政年份:
    2000
  • 资助金额:
    $ 9.22万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Isolation of human melanoma antigens recognized bt T cells for development of immunotherapy and gene therapy
分离人类黑色素瘤抗原识别的 BT T 细胞,用于开发免疫疗法和基因疗法
  • 批准号:
    10557083
  • 财政年份:
    1998
  • 资助金额:
    $ 9.22万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Isolation of human tumor antigens recognized by antibodies for development of immunotherapy and gene therapy
分离抗体识别的人类肿瘤抗原,用于开发免疫疗法和基因疗法
  • 批准号:
    10470264
  • 财政年份:
    1998
  • 资助金额:
    $ 9.22万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

相似海外基金

Spatial Profiling of Melanocytic Tumors and Their Microenvironment
黑素细胞肿瘤及其微环境的空间分析
  • 批准号:
    10729434
  • 财政年份:
    2023
  • 资助金额:
    $ 9.22万
  • 项目类别:
Lineage and transcriptional analysis of sacral neural crest contribution to the enteric nervous system
骶神经嵴对肠神经系统贡献的谱系和转录分析
  • 批准号:
    10679674
  • 财政年份:
    2023
  • 资助金额:
    $ 9.22万
  • 项目类别:
A role of balanced sex hormone in DNA repair in human melanocytes
平衡性激素在人类黑素细胞 DNA 修复中的作用
  • 批准号:
    10666307
  • 财政年份:
    2023
  • 资助金额:
    $ 9.22万
  • 项目类别:
Investigating the epidermal microenvironment in melanoblast migration and invasion: a novel approach to understanding invasive melanoma
研究黑色素细胞迁移和侵袭的表皮微环境:一种了解侵袭性黑色素瘤的新方法
  • 批准号:
    10537221
  • 财政年份:
    2023
  • 资助金额:
    $ 9.22万
  • 项目类别:
Hybrid approach for comprehensive mutation detection in a cell
用于细胞内全面突变检测的混合方法
  • 批准号:
    10662613
  • 财政年份:
    2023
  • 资助金额:
    $ 9.22万
  • 项目类别:
The Role of SoxE Transcription Factors in Neural Crest Cell Specialization
SoxE 转录因子在神经嵴细胞特化中的作用
  • 批准号:
    10662767
  • 财政年份:
    2023
  • 资助金额:
    $ 9.22万
  • 项目类别:
Harnessing the repetitive genome for cancer immunotherapy
利用重复基因组进行癌症免疫治疗
  • 批准号:
    10664127
  • 财政年份:
    2023
  • 资助金额:
    $ 9.22万
  • 项目类别:
Cellular and molecular regulators of melanocyte regeneration
黑素细胞再生的细胞和分子调节剂
  • 批准号:
    10659536
  • 财政年份:
    2023
  • 资助金额:
    $ 9.22万
  • 项目类别:
Dissecting a hormone-responsive processor for female activity and repetitive behavior
剖析女性活动和重复行为的激素反应处理器
  • 批准号:
    10796627
  • 财政年份:
    2023
  • 资助金额:
    $ 9.22万
  • 项目类别:
The Oncogene Activated Mitochondrial Unfolded Protein Response Regulates Senescence Biology
癌基因激活线粒体未折叠蛋白反应调节衰老生物学
  • 批准号:
    10598922
  • 财政年份:
    2023
  • 资助金额:
    $ 9.22万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了