Fundamental study for pathophysiology of human prostate hyperplasia in respect to gene expression profiles of proliferation/apoptosis related genes

人前列腺增生病理生理学增殖/凋亡相关基因表达谱的基础研究

• 批准号: 12470328
• 负责人: SUZUKI Kazuhiro
• 金额: $ 9.47万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470328/
• 关键词: prostate hypertrophy; prostate cancer; DNA microarray; gene expression profile; マイクロダイセクション; T7 RNA増幅法

DNA microarray technology provides us to analyze gene expression profiles of many genes using a small amount of samples. We performed DNA microarray analysis for understanding of pathophysiology of prostate diseases. Laser capture microdissection (LCM) enables to dissect epithelium and stroma separately, and we utilized LCM. In epithelial component, gene expression levels of E-cadherin, serine protease inhibitor, CD9, farnesyltransferase, cyclin 2 and cyclin 1 were increased significantly. On the other hand, gene expression levels of immunogloburin lambda locus, connective tissue growth factor, collagen type I, laminine alpha4, integrin alpha7, TGF-beta-induced (68 kDa) were significantly increased. Further studies for analysis of gene expression profiles of prostate cancer cells were performed. Molecular characterization after genistein or diethylstilbestrol treatment in human prostate cancer cells was investigated. Genistein induced glutathione peroxidase gene expression, and we thought that anti-tumor effect of genistein involved anti-oxidant effects.

DNA微阵列技术为我们提供了使用少量样本来分析许多基因的基因表达谱。为了了解前列腺疾病的病理生理学，我们进行了DNA微阵列分析。激光捕获显微切割技术(LCM)可以分离上皮和间质，我们使用了LCM。在上皮细胞中，E-钙粘蛋白、丝氨酸蛋白酶抑制物、CD9、法尼基转移酶、细胞周期蛋白2和细胞周期蛋白1的基因表达水平显著升高。另一方面，免疫球蛋白lambda位点、结缔组织生长因子、I型胶原、层粘连蛋白α4、整合素α7、转化生长因子-β(68 KDa)的基因表达水平显著升高。进一步对前列腺癌细胞的基因表达谱进行了分析。研究了金雀异黄素或己烯雌酚处理人前列腺癌细胞后的分子特征。金雀异黄素诱导谷胱甘肽过氧化物酶基因表达，我们认为金雀异黄素的抗肿瘤作用可能与抗氧化作用有关。

项目成果

Suzuki K., et al.: "Gene Expression Profiles in Human BPH: Utilization of Laser-Capture Microdissection and Quantitative Real-time PCR"Anti-Cancer Research. 21. 3861-3864 (2001)

Suzuki K. 等人：“人类 BPH 中的基因表达谱：激光捕获显微切割和定量实时 PCR 的利用”抗癌研究。

Suzuki K, et al.: "Gene expression profiles in human BPH : Utilization of laser-capture microdissection and quantitative real-time PCR"Anticancer-Reaserch. 21. 3861-3864 (2001)

Suzuki K 等人：“人类 BPH 中的基因表达谱：激光捕获显微切割和定量实时 PCR 的利用”抗癌研究。

Suzuki K, et al.: "Gene expression profiles in human BPH : Utilization of laser-capture microdissection and quantitative real-time PCR"Anticancer Research. 21. 3861-3864 (2001)

Suzuki K 等人：“人类 BPH 中的基因表达谱：激光捕获显微切割和定量实时 PCR 的利用”抗癌研究。