The roles of cofilin and LIM-kinase in chemotaxis of phagocytes
Cofilin 和 LIM 激酶在吞噬细胞趋化中的作用
基本信息
- 批准号:15590090
- 负责人:
- 金额:$ 1.98万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2003
- 资助国家:日本
- 起止时间:2003 至 2004
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Cofilin is a ubiquitous actin-binding protein. Only unphosphorylated cofilin binds actin and severs or depolymerizes a filamentous actin(F-actin) and cofilin is phosphrylated by LIM-kinase, a specific kinase of cofilin, to be an inactive form which does not bind actin. The phosphorylated cofilin is dephosphorylated by slingshot, a specific phosphatase, to be an active form. Recently we have reported that cofilin plays regulatory roles in superoxide production and phagocytosis by phagocytes. In this study, the roles of cofilin hi chemotaxis of leukocytes were investigated. Interleukin 8(IL-8), a potent physiological chemokine, triggered quick dephoshorylation and subsequent rephosphorylation of cofilin. The phosphorylation of cofilin was inhibited by S3-R peptide which consisted of a membrane-permeable peptide and a peptide of phosphorylation site of cofilin. When the S3-R peptide was introduced into neutrophil-like HL-60 cells, the chemotactic activity was enhanced while control peptide which contained an inverted sequence of phosphorylation site of cofilin did not such an enhancing effect. Wortmannin and LY294002,inhibitors of PI3 kinase, suppressed the chemotaxis and phosphorylation turnover of cofilin. On the other hand, siRNA of cofilin caused down-regulation of cofilin and inhibited the chemotaxis. Based on these results it is suggested that unphosphrylated active cofilin plays a critical role in the chemtaxis of phagocytes and PI3-kinase is involved in the control of phosphorylation/dephosphorylation cycle of cofilin.
Cofilin是一种普遍存在的肌动蛋白结合蛋白。只有未磷酸化的cofilin结合肌动蛋白并切断或解聚丝状肌动蛋白(F-肌动蛋白),并且cofilin被Cofilin的特异性激酶LIM-激酶磷酸化为不结合肌动蛋白的无活性形式。磷酸化的cofilin被特异性磷酸酶slingshot脱磷酸化,成为活性形式。最近,我们报道了cofilin在超氧化物的产生和吞噬细胞的吞噬作用中起调节作用。本研究探讨了cofilin在白细胞趋化性中的作用。白细胞介素8(IL-8)是一种强有力的生理趋化因子,可触发cofilin的快速去磷酸化和随后的再磷酸化。S3-R肽可抑制cofilin的磷酸化,S3-R肽由一个膜透性肽和一个cofilin磷酸化位点肽组成。将S3-R肽导入HL-60细胞后,其趋化活性增强,而含有cofilin磷酸化位点反向序列的对照肽则无此增强作用。PI 3激酶抑制剂Wortmannin和LY 294002可抑制cofilin的趋化性和磷酸化转换。另一方面,cofilin的siRNA引起cofilin的下调并抑制趋化性。这些结果表明,未磷酸化的活性cofilin在吞噬细胞的趋化过程中起着关键作用,而PI 3-激酶参与了cofilin磷酸化/去磷酸化循环的调控。
项目成果
期刊论文数量(21)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Tajima, K., Matsumoto, N., Ohmori, K., Wada, H., Ito, M., Suzuki, K., Yamamoto, K.: "Augmentation of NK cell-mediated cytotoxicity to tumor cells by inhibitory NK cell receptor blockers"Int.Immunol.. 16. 385-393 (2004)
Tajima, K.、Matsumoto, N.、Ohmori, K.、Wada, H.、Ito, M.、Suzuki, K.、Yamamoto, K.:“通过抑制性 NK 细胞增强 NK 细胞介导的对肿瘤细胞的细胞毒性
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Ribosomal protein S18 identified as a cofilin-binding protein by using phage display library.
通过噬菌体展示文库鉴定核糖体蛋白 S18 为肌丝蛋白丝切蛋白结合蛋白。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Kusui K.;Sasaki H.;Adachi R.;Matsui S.;Yamamoto K.;Yamaguchi T.;Kasahara T.;Suzuki K.
- 通讯作者:Suzuki K.
食細胞の機能発現とLIMキナーゼ-コフィリンによるアクチン細胞骨格制御
LIM 激酶-cofilin 吞噬细胞的功能表达和肌动蛋白细胞骨架的调节
- DOI:
- 发表时间:2003
- 期刊:
- 影响因子:0
- 作者:安達玲子;鈴木和博
- 通讯作者:鈴木和博
Kusui, K., Sasaki, H., Adachi, R., Matsui, S., Yamamoto, K., Yamaguchi, T., Kasahara, T., Suzuki, K.: "Ribosomal protein S18 identified as a cofilin-binding protein by using phage display library"Mol.Cell.Biochem.. 2004(印刷中).
Kusui, K.、Sasaki, H.、Adachi, R.、Matsui, S.、Yamamoto, K.、Yamaguchi, T.、Kasahara, T.、Suzuki, K.:“核糖体蛋白 S18 被鉴定为肌丝蛋白结合蛋白通过使用噬菌体展示文库“Mol.Cell.Biochem..2004(印刷中)”来分析蛋白质。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Relationship between phagocyte functions and regulation of actin cytoskeleton by LIM-kinase and cofilin (Review in Japanese)
吞噬细胞功能与 LIM 激酶和 cofilin 调节肌动蛋白细胞骨架的关系(日文综述)
- DOI:
- 发表时间:2003
- 期刊:
- 影响因子:0
- 作者:Adachi;R.;Suzuki;K.
- 通讯作者:K.
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SUZUKI Kazuhiro其他文献
A local currency system reflecting variety of values
反映多种价值的当地货币体系
- DOI:
- 发表时间:
2011 - 期刊:
- 影响因子:0
- 作者:
KINOSHITA Hirotsugu;TAJIMA Yoshiaki;KUBO Naoya;MORIZUMI Tetsuya;SUZUKI Kazuhiro - 通讯作者:
SUZUKI Kazuhiro
An electronic money system as substitute for banknotes
替代纸币的电子货币系统
- DOI:
- 发表时间:
2010 - 期刊:
- 影响因子:0
- 作者:
MORIZUMI Tetsuya;KUDO Mamoru;SUZUKI Kazuhiro;KINOSHITA Hirotsugu - 通讯作者:
KINOSHITA Hirotsugu
SUZUKI Kazuhiro的其他文献
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{{ truncateString('SUZUKI Kazuhiro', 18)}}的其他基金
Elucidation of the molecular basis of carbonyl stress related to social stress resilience
阐明与社会应激恢复相关的羰基应激的分子基础
- 批准号:
18K15354 - 财政年份:2018
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Novel mechanism for the regulation of lymphocyte dynamics
调节淋巴细胞动态的新机制
- 批准号:
24790470 - 财政年份:2012
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Effects of lifestyle education and physical fitness program during childhood and adolescence: follow-up study of Japanese children and adolescents
儿童期和青少年期生活方式教育和体适能计划的效果:日本儿童青少年的追踪研究
- 批准号:
23300224 - 财政年份:2011
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
The role of lipid metabolism in prostate cancer
脂质代谢在前列腺癌中的作用
- 批准号:
23390378 - 财政年份:2011
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Geology of the Proterozoic basement rocks and Permo-Triassic allochthons in the Geonggi massif, Korean Peninsula
朝鲜半岛京畿地块元古界基底岩及二叠纪-三叠纪异土地质
- 批准号:
21540471 - 财政年份:2009
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
New colored graphs towards a Brualdi-Hollingsworth conjecture
布鲁尔迪-霍林斯沃斯猜想的新彩色图表
- 批准号:
21740085 - 财政年份:2009
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Application and evaluation of educational practice for improving lifestyle and physical fitness program in Japanese school children
日本学童改善生活方式和体质计划教育实践的应用与评价
- 批准号:
20300205 - 财政年份:2008
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Genetic analysis of prostate cancer susceptibility performing genomewide homozygosity haplotype analysis
前列腺癌易感性的遗传分析进行全基因组纯合单倍型分析
- 批准号:
20390419 - 财政年份:2008
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
A substantial study about the practice model construction forcollaboration of community
社区协作实践模式构建实证研究
- 批准号:
20791755 - 财政年份:2008
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Reexamination of CHIME ages measured on rocks from orogenic belts in the Japanese Islands and the eastern margin of Asian Continent
日本列岛和亚洲大陆东缘造山带岩石CHIME年龄的复核
- 批准号:
19340149 - 财政年份:2007
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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Activation of calcineurin signal transduction by Clostridium perfingens beta-toxin in HL-60 cell
产气荚膜梭菌 β-毒素在 HL-60 细胞中激活钙调神经磷酸酶信号转导
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