CD14-dependent and independent pathways in B cells activation after stimulation with lipopolysaccharides from periodontopathic bacteria

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In this study we assessed the possible role of CD14 in B cells activation after stimulation with lipopolusaccharides from periodontopathic bacteria. We then examined the LPS-induced signal transduction mechanism that mediates later cellular responses of B cells. The results indicated that CD14 not restricted to myeloid dells, and was involved in some cellular activations and the isotype differentation to IgA-producing B cell to the enhanced expression of CDK TGF-β- production and the isotype different to IgA-producing B cells elicited by LPS. However, CD14-independent pathway could be also exist in the LPS-induced activation of B cells that eventually leads to proliferation, IL-6 production and the enhancement of IgM (but not IgA) secretion. It was also indicated that a trigger signal by the LPS from porphyromonas gingivalis as well as other periodontopathic bacteria, prevotella intermedia, Actinobacillus actinomycetemcomitans and Eikenella corrodens, could be transduced through both tyrosine and threonine phosphorylation pathways, and induced the activation of B cells that eventually leads to proliferation. Taken together, the present findings suggest to the LPSs from periodontopathic bacteria have a potent property to induce the stimulate of B cells through the CD14-dependent and independent activation pathways, in which increased tyrosine and threonine phosphorylation could be important signaling events that might lead to cellular responses.