Telomere DNA and Oral Tissue Senescence as Molecular Approach to Diagnosis and Treatment in Dentistry

端粒 DNA 和口腔组织衰老作为牙科诊断和治疗的分子方法

• 批准号: 12470414
• 负责人: KUMEI Yasuhiro
• 金额: $ 3.14万
• 依托单位: Tokyo Medical & Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470414/
• 关键词: telomere; senescence; bioinformatics; DNA; tissue regeneration; dentistry; 組織再生

We have developed a novel diagnostic system that is based on the evaluation of telomeres of DNA chromosomes in oral tissues including dental pulp and periodontal mucosa. The telomeres of most human somatic cells shorten with successive cell division. This method refers to the actual senescence of the oral tissue and to predict the post-treatment results. The sample DNA was extracted from each dental pulp and a small gingival piece. DNA was quantified with the fluorescent dye, using a commercially available human genomic DNA as a standardized control. Concentrations of the DNA samples were calculated from the equation describing the best-fit line generated with the standardized control DNA. After slot blot hybridization was performed, the telomere DNA was quantified. Results contributes to improvement of diagnostic accuracy treatment plan for individual patients.

我们已经开发了一种新的诊断系统，该系统是基于对口腔组织（包括牙髓和牙周粘膜）中DNA染色体端粒的评价。大多数人类体细胞的端粒随着连续的细胞分裂而缩短。这种方法是指实际衰老的口腔组织和预测治疗后的结果。从每个牙髓和一小块牙龈中提取样本DNA。使用市售的人基因组DNA作为标准化对照，用荧光染料定量DNA。根据描述标准化对照DNA生成的最佳拟合线的方程计算DNA样品的浓度。进行狭缝印迹杂交后，对端粒DNA进行定量。结果有助于提高诊断的准确性，治疗计划的个别患者。