Telomere DNA and Oral Tissue Senescence as Molecular Approach to Diagnosis and Treatment in Dentistry

我们开发了一种基于评估口腔组织（包括牙髓和牙周粘膜）DNA染色体端粒的新型诊断系统。大多数人类体细胞的端粒随着细胞的连续分裂而缩短。这种方法是根据口腔组织的实际衰老情况来预测治疗后的结果。从每个牙髓和一小块牙龈中提取DNA样本。用荧光染料定量DNA，使用市售的人类基因组DNA作为标准化对照。DNA样品的浓度由描述标准化对照DNA生成的最佳拟合线的方程计算。槽印迹杂交后，端粒DNA定量。结果有助于提高个别患者的诊断准确性和治疗方案。

We have developed a novel diagnostic system that is based on the evaluation of telomeres of DNA chromosomes in oral tissues including dental pulp and periodontal mucosa. The telomeres of most human somatic cells shorten with successive cell division. This method refers to the actual senescence of the oral tissue and to predict the post-treatment results. The sample DNA was extracted from each dental pulp and a small gingival piece. DNA was quantified with the fluorescent dye, using a commercially available human genomic DNA as a standardized control. Concentrations of the DNA samples were calculated from the equation describing the best-fit line generated with the standardized control DNA. After slot blot hybridization was performed, the telomere DNA was quantified. Results contributes to improvement of diagnostic accuracy treatment plan for individual patients.