A study of interstitial ihermoradiotherapy using powdered ferromagnetic body for oral cancer.

铁磁体粉体间质热放射治疗口腔癌的研究。

• 批准号: 12470435
• 负责人: TOHNAI Iwai
• 金额: $ 4.74万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470435/
• 关键词: Oral cancer; Hyperthermia; Interstitial hyperthermia; Magnetite induction hyperthermia; Radiotherapy; Thermoradiotherapy; Magnetite Cationic Liposomes (MCLs); 組織内温熱療法; 微粉末磁性体; マグネタイトカチオニックリポソーム(MCL_s); 磁場誘導組織内加温法; 舌癌; VX-7移植舌腫瘍; マグネチックカチオニックリ

The anti-tumor effects of the interstitial hyperthermia using MCLs was evaluated in VX7 transplanted tumor of rabbits. MCLs of 0.4 ml were injected into the tongue tumor of 10 mm. The tumor was heated for 30 min. Control was not heated, but injected MCLs only. In the experimental rabbits, the temperature in the tumor was as high as 43.0 ゜C. The tumor volumes of heated rabbits were significantly smaller than those of the non heated ones. The tongues, removed from heated rabbits on 4 weeks after hyperthermia, showed no tumor cells. The thermoradiotherapy was evaluated, when the MCLs is not full in the tumor, next. MCLs of 0.2 ml were injected into the tongue tumor of 10 mm. The tumor was heated for 30 min. The rabbit in group I were performed hyperthermia only, those in group II were irradiation only, those in group III were combined therapy with hyperthennia and radiotherapy(10Gy), and those in group IV were non treatment as control. As the results, the anti-tumor effects was observed in group III of thermoradiotherapy. The results was very important in clinical. The present study confirmed the effectiveness of interstitial hyperthermia using MCLs for oral cancer.

采用兔VX7移植瘤模型，观察微循环激光间质热疗的抗肿瘤作用。将0.4 ml MCL注射到10 mm的舌肿瘤中。肿瘤加热30 min。对照组不加热，但仅注射MCL。在实验兔中，肿瘤内的温度高达43.0 ℃。加热组肿瘤体积明显小于未加热组。热疗后4周，从加热的兔子身上取下的舌头没有显示出肿瘤细胞。当肿瘤中的MCL不充分时，再进行热放疗。将MCL0.2ml注入10mm的舌肿瘤内，加热30min，I组单纯热疗，II组单纯放疗，III组热疗加放疗（10Gy），IV组不治疗作为对照。结果表明，热放疗Ⅲ组有明显的抗肿瘤作用。其结果在临床上具有重要意义。本研究证实了使用MCL的间质热疗对口腔癌的有效性。

项目成果

12. Tohnai I,Hayashi Y,Mitsudo K,Shigetomi T,Ueda M,Ishigaki T: "Prognostic Evaluation of Preoperaive Thermochemoradiotherapy for N3 Cervical Lymph Node Metastases of Oral Cancer"Oncology. in press.

12. Tohnai I，Hayashi Y，Mitsudo K，Shigetomi T，Ueda M，Ishigaki T：“口腔癌 N3 颈淋巴结转移的术前热放化疗的预后评估”肿瘤学。

光籐健司: "温熱刺激におけるマウス舌粘膜上皮のランゲルハンス細胞とThy-1陽性細胞Tリンパ球の免疫組織化学的研究"日本口腔外科学会雑誌. 47・2. 83-88 (2001)

Kenji Koto：“热刺激下小鼠舌粘膜上皮中朗格汉斯细胞和 Thy-1 阳性 T 淋巴细胞的免疫组织化学研究”日本口腔颌面外科杂志 47・2（2001 年）。

Matsuno H: "Interstitial Hyperthermia Using Magnetite Cationic Liposomes Inhibit to Tumor Growth of VX-7 Transplanted Tumor in Rabbit Tongue"Japanese Journal of Hyperthermic Oncology. 17(3). 141-150 (2001)

Matsuno H：“使用磁铁矿阳离子脂质体的间质热疗抑制兔舌中 VX-7 移植肿瘤的生长”日本热肿瘤学杂志。

7.Shinkai M,Biao Le,Honda H,Yoshikawa K,Shimizu K,Saga S,Wakabayashi T,Yohida J and Kobayashi T: "Targeting Hyperthermia for Renal Cell Carcinoma UsingHumanMNAntgen specific Magntoliposomes"Jpn.J.Cancer Res. 92. 1138-1145 (2001)

7.Shinkai M、Biao Le、Honda H、Yoshikawa K、Shimizu K、Saga S、Wakabayashi T、Yohida J 和 Kobayashi T：“使用人 MNA 抗原特异性磁脂质体靶向热疗治疗肾细胞癌”Jpn.J.Cancer Res。

Bouhon I: "Heat Induction of reporter gene expression via the gadd 153 promoter and its possible application to hyperthermia treatment of cancer"Cytechnology. 33. 131-137 (2000)

Bouhon I：“通过 gadd 153 启动子热诱导报告基因表达及其在癌症热疗治疗中的可能应用”Cytechnology。