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Phytoremediation by transgenic plants with enhanced ability of sulfur assimilation

Phytoremediation by transgenic plants with enhanced ability of sulfur assimilation
具有增强硫同化能力的转基因植物的植物修复
批准号:
12470510
负责人:
SAITO Kazuki
金额:
$8.51万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2000
资助国家:
日本
项目状态:
已结题
起止时间:
2000 至 2002

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中文摘要
翻译
含硫化合物对环境的污染是全球环境面临的严重问题。特别是气态SO2,主要由自然来源和人类活动排放,即化石燃料的燃烧、含硫矿石的工业精炼等,对人类健康和全球动植物生态系统都有影响。利用植物从环境中去除污染物的植物修复技术受到了广泛的关注。半胱氨酸合成酶[ο-乙酰-L-丝氨酸(硫醇)-裂解酶,EC 4.2.99.8](Csase)负责半胱氨酸生物合成的最后一步。利用胞质Csase和叶绿体Csase两个转化子进行杂交,获得了胞质Csase活性和叶绿体Csase活性均较高的烟草转基因植株(F1)。转基因F_1代植株对有毒的二氧化硫和亚硫酸盐具有高度的耐受性。熏蒸时用0。LμL1<-1>二氧化硫处理后,F_1代植株叶片中的半胱氨酸和谷胱甘肽含量均显著增加,而未转化对照植株叶片中的半胱氨酸和谷胱甘肽含量没有显著增加。此外,F_1代植株叶片对产生活性氧的除草剂百草枯的抗性增强。还获得了高表达丝氨酸乙酰转移酶的转基因拟南芥植株,丝氨酸乙酰转移酶是硫同化的关键调节酶,用来检测对重金属Cd等的抗性。
英文摘要
Environmental pollution by sulfur-containing compounds is a serious problem for the global environment. In particular, gaseous SO_2, which is emitted mainly by both natural sources and human activities, I. E. combustion of fossil fuels, industrial refining of sulfur-containing ores, influences human health and the global ecological system of animals and plants. Phytoremediation, which uses plants to remove pollutants from the environment, has received wide attention. Cysteine synthase [ο-acetyl-L-serine(thiol)-lyase, EC 4.2.99.8] (Csase) is responsible for the final step in biosynthesis of cysteine. Transgenic tobacco(Nicotiana tabacum) (F_1)plants with enhanced Csase activities both in the cytosol and in the chloroplasts were generated by cross-fertilization of two transformants expressing either cytosolic Csase or chloroplastic Csase. The F_1 transgenic plants were highly tolerant to toxic sulfur dioxide and sulfite. Upon fumigation with 0. lμLL^<-1> sulfur dioxide, both the cysteine and glutathione contents in leaves of F_1 plants were increased significantly, but not in leaves of non-transformed control plants. Furthermore, the leaves of F_1 plants exhibited the increased resistance to paraquat, a herbicide generating active oxygen species. The transgenic Arabidopsis plants over expressing serine acetyltransferase, a key regulatory enzyme of sulfur assimilation, were also generated to examine the resistance to a heavy metal, Cd etc.
期刊论文(26)
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会议论文
Masaaki Noji, et al.: "Cysteine synthase overexpression in tobacco confers tolerance to sulfur-containing environmental pollutants"Plant Physiol.. 126. 973-980 (2001)
Masaaki Noji 等人:“烟草中半胱氨酸合成酶的过度表达赋予对含硫环境污染物的耐受性”Plant Physiol.. 126. 973-980 (2001)
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Masaaki Noji, et al.: "Molecular and biochemical analysis of serine acetyltransferase and cysteine synthase towards sulfur metabolic engineering in plants"Amino Acids. 22. 231-243 (2002)
Masaaki Noji 等人:“植物中硫代谢工程的丝氨酸乙酰转移酶和半胱氨酸合酶的分子和生化分析”氨基酸。
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通讯作者:
Masaaki Noji, Maiko Saito, Michimi Nakamura, Mitsuyo Aono, Hikaru Saji and Kazuki Saito: "Cysteine synthase overexpression in tobacco confers tolerance to sulfur-containing environmental pollutants"Plant Physiol. 126. 973-980 (2001)
Masaaki Noji、Maiko Saito、Michimi Nakamura、Mitsuyo Aono、Hikaru Saji 和 Kazuki Saito:“烟草中半胱氨酸合酶的过度表达赋予对含硫环境污染物的耐受性”Plant Physiol。
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Masami Yokota Hirai, Toru Fujiwara, Motoko Awazuhara, Tomoko Kimura, Masaaki Noji and Kazuki Saito: "Global expression profiling of sulfur-starved Arabidopsis by DMA macroarray reveals the role of ο-acetyl-L-serine as a general regulator of gene expressio
Masami Yokota Hirai、Toru Fujiwara、Motoko Awazuhara、Tomoko Kimura、Masaaki Noji 和 Kazuki Saito:“通过 DMA 宏阵列对硫饥饿的拟南芥进行全局表达谱分析,揭示了 ο-乙酰基-L-丝氨酸作为基因表达通用调节因子的作用。
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13
    Establishment of molecular basis on ligand recognition by the insect prothoracicotropic hormone receptor
    Systematic structure optimization of the EGF receptor-inhibiting peptide through use of a screening technology
    • 批准号:
      24590134
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $3.49万
    • 财政年份:
      2012
    • 负责人:
      SAITO Kazuki
    • 依托单位:
    Integrated analysis of transcriptome and metabolome in Japanese medicinal plants
    • 批准号:
      24390030
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $11.65万
    • 财政年份:
      2012
    • 负责人:
      SAITO Kazuki
    • 依托单位:
    Development of peptide inhibitors against the EGF receptor based on its structural properties.
    海外基金