Transgenic Plants Absorbing Environmental Sulfur Pollutants

吸收环境硫污染物的转基因植物

• 批准号: 08557127
• 负责人: SAITO Kazuki
• 金额: $ 3.78万
• 依托单位: CHIBA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08557127/
• 关键词: Genetic engineering; Environment; Moleculart cloning; Sulfur resistance; Sulfur dioxide; Sulfur metabolism; Acid rain; クローニング

Cysteine synthase [O-acetyl-L-serine(thiol)-lyase, EC 4.2.99.8] (CSase), which is responsible for the final step in biosynthesis of cysteine, catalyzes the formation of L- cysteine from O-acetyl-L-serine and hydrogen sulfide. Transgenic tobacco plants carrying either spinach cytosolic Csase A cDNA (3F) or chimeric CSase A cDNA fused with the sequence for chloroplast-targeting transit peptide of pea ribulose- 1,5- bisphosphate carboxylase small subunit (4F) driven by the cauliflower mosaic virus 35S promoter have already been constructed. The enhanced CSase activities of transgenic plants of 3F and 4F have been shown in cytosol and in chloroplasts, respectively (1). We crossed 3F transgenic tobacco with 4F transgenic tobacco, and generated F1 transgenic tobacco in which Csase activities were enhanced both in cytosol and chloroplasts. Both cysteine and reduced glutathione (GSH) contents in leaves of F1 transformants were higher than those of 3F, 4F transformants and control plants, indicating that high activities of CSase both in cytosol and chloroplasts caused an efficient assimilation of sulfur into cysteine and GSH.Furthermore, F1 transgenic tobacco showed more resistance to sulfur dioxide (SO2) gas and sodium sulfite than 3F, 4F transformants and control tobacco.

半胱氨酸合酶[0-乙酰基-L-丝氨酸（硫醇）-裂解酶，EC 4.2.99.8]（CSase）负责半胱氨酸生物合成的最后一步，催化由0-乙酰基-L-丝氨酸和硫化氢形成L-半胱氨酸。已经构建了携带菠菜胞质Csase A cDNA（3F）或嵌合Csase A cDNA的转基因烟草植物，所述嵌合Csase A cDNA与由花椰菜花叶病毒35 S启动子驱动的豌豆核酮糖-1，5-二磷酸羧化酶小亚基（4F）的叶绿体靶向转运肽序列融合。3F和4F的转基因植物的CSase活性增强已分别在细胞质和叶绿体中显示（1）。将3F转基因烟草与4F转基因烟草杂交，获得了F1代转基因烟草，其胞质和叶绿体中的Csase活性都得到了提高。F1代转基因烟草叶片中半胱氨酸和还原型谷胱甘肽（GSH）含量均高于3F、4F和对照，表明细胞质和叶绿体中CSase活性高，硫被有效同化为半胱氨酸和GSH，且F1代转基因烟草对SO2气体和亚硫酸钠的抗性高于3F，4F转化体和对照烟草。

项目成果

Hideki Takahashi, Mami Yamazaki, Noriko Sasakura, Akiko Watanabe, Thomas Leustek, Janice de Almeida Engler, Gilbert Engler, Marc Van Montagu and Kazuki Saito: "Regulation of sulfur assimilation in higher plants : A sulfate transporter induced in sulfate-s

Hideki Takahashi、Mami Yamazaki、Noriko Sasakura、Akiko Watanabe、Thomas Leustek、Janice de Almeida Engler、Gilbert Engler、Marc Van Montagu 和 Kazuki Saito：“高等植物中硫同化的调节：硫酸盐诱导的硫酸盐转运蛋白

Kazuki Saito: "Molecular aspects of sulfur assimilation and acclimation to sulfur supply in plants" Stress Responses of Photosynthetic Organisms. K.Satoh and N.Murata, eds.(Elsevier Science B.V., Amsterdam). 215-226 (1998)

Kazuki Saito：“植物中硫同化和适应硫供应的分子方面”光合生物的应激反应。

Kazuki Saito, et.al.: "Genomic structure and expression analyses of serine acetyltransferase gene in citrullus vulqaris(watermelon)" Gene. 189. 57-63 (1997)

Kazuki Saito 等：“西瓜丝氨酸乙酰转移酶基因的基因组结构和表达分析”基因。

M Noji: "Isoform-dependent differences in feedback regulation and subcellular localization of serine acetyltransferase involved in cysteine biosynthesis from Arabidopsis thaliana." J.Biol.Chem.273. 32739-32745 (1998)

M Noji：“参与拟南芥半胱氨酸生物合成的丝氨酸乙酰转移酶的反馈调节和亚细胞定位存在异构体依赖性差异。”

Mami Yamazaki,et.al.,: "Transgenic fertile Scoparia dulcis L.,a folk medicinal plant,conterred with a herbicide-resistant trait using an Ri binary vector" Plant Cell Reports. 15. 317-321 (1996)

Mami Yamazaki 等人：“转基因可育 Sco​​paria dulcis L.，一种民间药用植物，使用 Ri 二元载体对抗除草剂抗性性状”《植物细胞报告》。