Molecular Biology and Engineering of Biosynthesis of Sulfur-containing Metabolites in Plants

植物含硫代谢物生物合成的分子生物学与工程

基本信息

  • 批准号:
    08458170
  • 负责人:
  • 金额:
    $ 4.42万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    1996
  • 资助国家:
    日本
  • 起止时间:
    1996 至 1998
  • 项目状态:
    已结题

项目摘要

Cysteine is the first organic precursor for the formation of sulfur-containing metabolites in plants such as methionine and glutathione. Two regulatory mechanisms for cysteine formation have been clarified by our recent studies ; one at the level of enzyme activity and the other at the steady-state mRNA level. The enzyme activity of serine acetyltransferase catalyzing the formation of O -acetylserine from serine and acetyl - CoA is inhibited by L-cysteine at the concentration of less 10 _ M.This inhibition was observed in the isoforms from watermelon, spinach and Arabidopsis thaliana. However, two isozymes from A.thaliana presumably localizing in organelles were insensitive to L-cysteine, suggesting different regulation among isozymes of different subcellular localization. Northern hybridization analysis of sulfate starved plants indicated that the steady-state mRNA level of an isoform of sulfate transporter (AST68) increased specifically in roots up to -9 folds by sulfate starvation Among all the structural genes encoding the proteins for sulfate assimilation, sulfate transporter (AST68) and APS reductase are inducible by sulfate starvation in A.thaliana. The experiments of in situ hybridization and transgenic plants with the promoter-gus fusion indicated that AST68 is expressed in the central cylinder of roots and the vascular tissues of shoots. The transgenic plants of AST68 anti-sense construct exhibited sulfur deficient symptom in cotyledons and also tolerance to selenate. These results indicating that AST68 plays a central role in sulfate assimilation in A.thaliana. The cDNA encoding alliinase responsible for conversion of alliin to sulfenic acid was isolated from A Ilium tuberosum. The recombinant protein expressed in yeast cells exhibited the alliinase activity.
半胱氨酸是植物中形成含硫代谢物如蛋氨酸和谷胱甘肽的第一个有机前体。半胱氨酸形成的两种调节机制已被我们最近的研究所阐明;一种是在酶活性水平,另一种是在稳态mRNA水平。丝氨酸乙酰转移酶催化丝氨酸和乙酰辅酶A形成O -乙酰丝氨酸的酶活性在小于10 μ M的浓度下被L-半胱氨酸抑制。在来自西瓜、菠菜和拟南芥的同工型中观察到这种抑制。然而,两种可能定位于细胞器的拟南芥同工酶对L-半胱氨酸不敏感,表明不同亚细胞定位的同工酶之间存在不同的调节。北方杂交分析表明,稳态mRNA水平的硫酸盐转运蛋白(AST 68)的异构体增加,特别是在根中的硫酸盐饥饿高达-9倍。在所有的结构基因编码的蛋白质的硫酸盐同化,硫酸盐转运蛋白(AST 68)和APS还原酶是诱导硫酸盐饥饿在拟南芥。原位杂交和转基因植株的实验表明,AST 68基因在根的中心柱和茎的维管组织中表达。AST 68反义构建体的转基因植株表现出子叶缺硫症状,同时也表现出对硒的耐受性。这些结果表明,AST 68起着核心作用,在拟南芥的硫酸盐同化。从马铃薯中分离到编码蒜氨酸酶的cDNA,该酶负责蒜氨酸转化为次磺酸。重组蛋白在酵母细胞中的表达具有蒜氨酸酶活性。

项目成果

期刊论文数量(12)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Hideaki Takahashi, Mami Yamazaki, Noriko Sasakura, Akiko Watanabe, Thomas Leustek, Janice de Almeida Engler, Gilbert Engler, Marc Van Montagu and Kazuki Saito: "Regulation of sulfur assimilation in higher plants : A sulfate transporter induced in sulfate-
Hideaki Takahashi、Mami Yamazaki、Noriko Sasakura、Akiko Watanabe、Thomas Leustek、Janice de Almeida Engler、Gilbert Engler、Marc Van Montagu 和 Kazuki Saito:“高等植物中硫同化的调节:在硫酸盐中诱导的硫酸盐转运蛋白
  • DOI:
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  • 期刊:
  • 影响因子:
    0
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Kazuki Saito, et al.,: "Production of plant non-protein amino acids by recombinant enzymes of sequential biosynthetic reactions in bacteria" Biol.Pharm.Bull.20. 47-53 (1997)
Kazuki Saito 等人:“通过细菌中连续生物合成反应的重组酶生产植物非蛋白质氨基酸”Biol.Pharm.Bull.20。
  • DOI:
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  • 影响因子:
    0
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  • 通讯作者:
M Noji: "Isoform-dependent differences in feedback regulation and subcellular localization of serine acetyltransferase involved in cysteine biosynthesis from Arabidopsis thaliana." J.Biol.Chem.273. 32739-32745 (1998)
M Noji:“参与拟南芥半胱氨酸生物合成的丝氨酸乙酰转移酶的反馈调节和亚细胞定位存在异构体依赖性差异。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
Tomotumi MANABE et al.: "Alliinase[S-alk(en)yl-L-cysteine sulfoxide lyase]from Allium tuberosum (Chinese chive)" Eur.J.Biochem.257. 21-30 (1998)
Tomotumi MANABE 等人:“来自葱的蒜氨酸酶[S-烷(烯)基-L-半胱氨酸亚砜裂解酶]”Eur.J.Biochem.257。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
C-L.Ho: "Molecular characterization of plastidic phosphoserine aminotransferase in serine biosynthesis from Arabidopsis." Plant J.16. 443-452 (1998)
C-L.Ho:“拟南芥丝氨酸生物合成中质体磷酸丝氨酸转氨酶的分子特征。”
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  • 影响因子:
    0
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SAITO Kazuki其他文献

SAITO Kazuki的其他文献

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{{ truncateString('SAITO Kazuki', 18)}}的其他基金

Establishment of molecular basis on ligand recognition by the insect prothoracicotropic hormone receptor
昆虫促前胸腺激素受体识别配体的分子基础的建立
  • 批准号:
    15K14720
  • 财政年份:
    2015
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Systematic structure optimization of the EGF receptor-inhibiting peptide through use of a screening technology
利用筛选技术系统优化EGF受体抑制肽的结构
  • 批准号:
    24590134
  • 财政年份:
    2012
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Integrated analysis of transcriptome and metabolome in Japanese medicinal plants
日本药用植物转录组和代谢组的综合分析
  • 批准号:
    24390030
  • 财政年份:
    2012
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of peptide inhibitors against the EGF receptor based on its structural properties.
根据 EGF 受体的结构特性开发肽抑制剂。
  • 批准号:
    21590129
  • 财政年份:
    2009
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Phytochemical genomics : Genomic basis for phytochemical diversity
植物化学基因组学:植物化学多样性的基因组基础
  • 批准号:
    19201043
  • 财政年份:
    2007
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Metabolomics-based post-genome science in plants
基于代谢组学的植物后基因组科学
  • 批准号:
    15201045
  • 财政年份:
    2003
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Molecular metabolic engineering of clinically-used anti-cancer plant products
临床抗癌植物产品的分子代谢工程
  • 批准号:
    12558075
  • 财政年份:
    2000
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Phytoremediation by transgenic plants with enhanced ability of sulfur assimilation
具有增强硫同化能力的转基因植物的植物修复
  • 批准号:
    12470510
  • 财政年份:
    2000
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular regulation of sulfur transport, assimilation and conversion in storage function in plants
植物储存功能中硫运输、同化和转化的分子调控
  • 批准号:
    12138202
  • 财政年份:
    2000
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
Transgenic Plants Absorbing Environmental Sulfur Pollutants
吸收环境硫污染物的转基因植物
  • 批准号:
    08557127
  • 财政年份:
    1996
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

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Finding the targets of natural products in complex botanical extracts.
寻找复杂植物提取物中天然产物的靶标。
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通过大数据分析和生物合成研究获取和扩大天然产物化学多样性
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