Regulation of meiotic chromosome segregation by cohesin Rec8

粘连蛋白 Rec8 对减数分裂染色体分离的调节

• 批准号: 12480207
• 负责人: WATANABE Yoshinori
• 金额: $ 10.56万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12480207/
• 关键词: chromosome cohesion factor; centromere; heterochromatin; HP1; Swi6; equational division; Rec8; Rad21; 還元分裂; 減数分裂

Fission yeast centromeres, like that of higher eukaryotes, are comprised of repeated DNA structures and associated heterochromatin protein complexes, that play a critical role in faithful segregation of chromosomes during cell division. Cohesin protein complexes, which are essential for sister chromatid cohesion and proper chromosome segregation, are enriched at centromeric repeats. We have identified functional and physical link, between heterochromatin and cohesin. We find that the preferential localization of cohesins at the centromeric repeats is dependent on Swi6, a conserved heterochromatin protein that is required for proper kinetochore, function. Cohesin is also enriched at the mating type heterochromatic region in a Swi6 dependent manner and is required to preserve genomic integrity of this locus. We provide evidence that a cohesin subunit Psc3 interacts with Swi6 and its mouse homolog HP1. These data define a conserved function of Swi6/HP1 in recruitment of cohesin to heterochromatic regions, promoting proper segregation of chromosomes.

与高等真核生物一样，分裂酵母着丝粒由重复的DNA结构和相关的异染色质蛋白复合物组成，在细胞分裂过程中染色体的忠实分离中起着关键作用。内聚蛋白复合物是姐妹染色单体内聚和染色体正确分离所必需的，它在着丝粒重复上富集。我们已经确定了异染色质和内聚蛋白之间的功能和物理联系。我们发现内聚蛋白在着丝粒重复序列上的优先定位依赖于Swi6，这是一种保守的异染色质蛋白，是着丝粒正常功能所必需的。内聚蛋白也以Swi6依赖的方式富集在交配型异色区，这是保持该位点基因组完整性所必需的。我们提供的证据表明，一个内聚蛋白亚基Psc3与Swi6及其小鼠同源物HP1相互作用。这些数据确定了Swi6/HP1在募集内聚蛋白到异染色质区，促进染色体适当分离方面的保守功能。

项目成果

Sato, M., Watanabe, Y., Akiyoshi, Y., Yarnamoto, M.: "14-3-3 protein interferes with the binding of RNA to the phosphorylated form of fission yeast meiotic regulator Mei2n"Curr. Biol.. 12. 141-145 (2002)

Sato, M.、Watanabe, Y.、Akiyoshi, Y.、Yarnamoto, M.：“14-3-3 蛋白干扰 RNA 与裂殖酵母减数分裂调节因子 Mei2n 磷酸化形式的结合”Curr。

Higuchi, T., Watanabe, Y., Yamamoto, M.: "Protein kinase A regulates sexual development and gluconeogenesis through phosphorylation of a Zn-finger transcriptional activator Rst2p in fission yeast"Mol.Cell.Biol.. 22. 1-11 (2002)

Higuchi, T.、Watanabe, Y.、Yamamoto, M.：“蛋白质激酶 A 通过裂殖酵母中锌指转录激活因子 Rst2p 的磷酸化调节性发育和糖异生”Mol.Cell.Biol.. 22. 1-11

Sato, M., Shinozaki-Yabana, S., Yamashita A., Watanabe, Y., Yamamoto, M.: "The fission yeast meiotic regulator Mei2p undergoes nucleocytoplasmic shuttling"FEBS Lett.. 499. 251-255 (2001)

Sato, M.、Shinozaki-Yabana, S.、Yamashita A.、Watanabe, Y.、Yamamoto, M.：“裂变酵母减数分裂调节因子 Mei2p 经历核细胞质穿梭”FEBS Lett.. 499. 251-255 (2001)

Ura,S.: "MST1-JNK promotes apoptosis via caspase-dependent and-independent pathways"Genes to Cells. (in press). (2001)

Ura,S.：“MST1-JNK 通过半胱天冬酶依赖性和独立途径促进细胞凋亡”基因到细胞。

Kitanura, K., Katayama, S., Dhut, S., Sato, M., Watanabe, Y., Yamamoto, M., Toda, T.: "Phosphorylation of Mei2 and Stell by Pat1 Kinase Inhibits Sexual Differentiation via Ubiquitin Proteolysis and 14-3-3 Protein in Fission Yeast"Dev.Cell. 1. 389-399 (200

Kitanura, K.、Katayama, S.、Dhut, S.、Sato, M.、Watanabe, Y.、Yamamoto, M.、Toda, T.：“Pat1 激酶对 Mei2 和 Stell 的磷酸化通过泛素蛋白水解抑制性别分化