Development of hybrid bio-fuelcell system using biomass

利用生物质开发混合生物燃料电池系统

• 批准号: 12555230
• 负责人: NISHIO Naomichi
• 金额: $ 8.26万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12555230/
• 关键词: hydrogen; methane; two-step process; biomass; UAFP reactor; UASB reactor; fuel cell; facultative annerobe; 生ゴミ; Enterobacter aerogenes; グリセロール; キシロース

In order to utilize biomass effectively, Bio-fuel cell system was studied. In this process, hydrogen which is direct substrate for fuel cell was firstly produced in hydrogen-acid fermentation by pure culture of microorganism or by microbial consortia, and then the produced acids and alcohols in wastewater was converted to methane by methane fermentation such as UASB aad UAFP reactor. At first, facultative microorganism that had high productivity of hydrogen was searched, and Enterobacter aerogenes HU-101 was found as the suitable microorganism for hydrogen production from glucose. Hydrogen production by this bacterium depended on redox state of substrates. The higher redox state of substrate gave, the higher hydrogen production. Besides hydrogen, this bacterium produced ethanol, 2,3-butanediol, acetate and lactate, which were able to be used for methane production with UASB reactor. Furthermore, It was tested whether this hydrogen-methane two-stage process would be applied to actual biomass such as gavages and bread manufacture wastes. As the results of several experiments, it was concluded that the developed two-stage process was really applicable to actual organic wastes.

为了有效地利用生物质，对生物燃料电池系统进行了研究。该工艺首先利用纯培养的微生物或微生物菌群进行氢酸发酵生产氢气，然后利用UASB和UAFP反应器将废水中产生的酸和醇转化为甲烷。首先，寻找具有高产氢能力的兼性微生物，并发现产气肠杆菌HU-101是用于从葡萄糖生产氢的合适微生物。该菌产氢依赖于底物的氧化还原状态。底物的氧化还原态越高，产氢量越高。该菌除产氢外，还能产乙醇、2，3-丁二醇、乙酸和乳酸，可用于UASB反应器产甲烷。此外，还测试了这种氢-甲烷两段工艺是否适用于实际的生物质，如灌胃和面包制造废物。实验结果表明，两段法处理实际有机废弃物是可行的。

项目成果

H.Nagai, M.Kobayashi, Y.Tsuji, Y.Nakashimada, T.Kakizono, N.Nishio: "Biological and chemical treatment of the solid waste from the process of soy sauce manufacture"Water Sciences and Technology. 45・12. 335-338 (2002)

H.Nagai、M.Kobayashi、Y.Tsuji、Y.Nakashimada、T.Kakizono、N.Nishio：“酱油制造过程中固体废物的生物和化学处理”水科学与技术45・12。 335-338 (2002)

Y.Nakashimada, M.A.Rachman, T.Kakizono, N.Nishio: "H_2 production of Enterobacter aero genes altered by extracellular and intracellular redox states"International Journal of Hydrogen Energy. 27. 1399-1405 (2002)

Y.Nakashimada、M.A.Rachman、T.Kakizono、N.Nishio：“胞外和胞内氧化还原状态改变的产气肠杆菌基因的 H_2 生产”国际氢能杂志。

N.Nishio, Y.Nakashimada: "Hydrogen-methane two-stage process for anaerobic wastewater treatment"Biotechnology for sustainable utilization of biological resources in the tropics. 16(印刷中). (2003)

N.Nishio，Y.Nakashimada：“厌氧废水处理的氢-甲烷两阶段工艺”热带生物资源可持续利用的生物技术16（印刷中）。

Y.Nakashimada, M.A.Rachman, T.Kakizono, N.Nishio: "H_2 production of Enterobacter aerogenes altered by extracellular and intracellular redox states"International Journal of Hydrogen Energy. 27・11/12. 1399-1405 (2002)

Y.Nakashimada、M.A.Rachman、T.Kakizono、N.Nishio：“细胞外和细胞内氧化还原状态改变产气肠杆菌的 H_2 生产”国际氢能杂志 27・11/12（2002）。

H.Nagai, M.Kobayashi, Y.Tsuji, Y.Nakashimada, T.Kakizono, N.Nishio: "Biological and chemical treatment of the solid waste from the process of soy sauce manufacture"Water Science and Technology. 45. 335-338 (2002)

H.Nagai、M.Kobayashi、Y.Tsuji、Y.Nakashimada、T.Kakizono、N.Nishio：“酱油制造过程中固体废物的生物化学处理”水科学与技术。