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Anaerobic digestion and energy recovery of high salt solid wastes using marine methanogens

海洋产甲烷菌对高盐固体废物的厌氧消化及能量回收

基本信息

批准号：
12839011
负责人：
NISHIO Naomichi
金额：
$ 2.3万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12839011/
关键词：
Marine sediments Methane halotolerance Methanogen Acetic acid Methanosaeta UASB reactor soy sauce refuse 低級脂肪酸集積培養ギ酸 Methanocalculus

项目摘要

When marine sediment collected from Hiroshima Bay was cultured in artificial seawater containing 3% NaCl at 37 C, active methane production was observed. The well-acclimated methanogens could grow at even 9% NaCl. Novel halo-tolerant acetoclastic methanogen was estimated to belong to Methanosaeta sp. based on rDNA sequence analysis since the homology of 16S rDNA between isolated methanogen and Methanosaeta concilii was 90% but it might be new genera. The acclimated acetoclastic methanogens was applied to continuous methane fermentation of acetate with an UASB reactor, acetate (77 mM from 200g wet mud) which was released from organic matters in sea sediment mud by anaerobic treatment was successfully converted to CH_4 giving 90 mM/d at 3.0 d^<-1> of dilution rate. Furthermore, using marine sediments as culture seed, soybean solid waste (25g wet/l) was converted to CH_4 at 25 mmol/m^3/d of maximum CH_4 production rate giving 15% solid reduction after 30 d, indicating that for the treatment of soybean solid waste, two step methane fermentation process consisting of hydrolytic and acid formation reactor, and methane formation reactor in which halo-tolerant methanogen can be used would be feasible for more efficient solid reduction.

从广岛湾采集的海洋沉积物在37℃含3%氯化钠的人工海水中培养时，观察到活性甲烷的产生。驯化良好的产甲烷菌即使在9%的氯化钠下也能生长。新的耐晕的乙酰裂解产甲烷菌被鉴定为甲烷菌属。根据rDNA序列分析，分离到的产甲烷菌与致密甲烷杆菌的16S rDNA序列同源性为90%，但可能是新属。将驯化后的裂解产甲烷菌应用于UASB反应器进行乙酸酯甲烷连续发酵，成功地将厌氧处理后的海洋底泥中有机物释放出的乙酸乙酯(200 g湿泥中77 mm)转化为CH4，稀释率为3.0d^~(-1)~(-1)。此外，以海洋沉积物为培养种子，大豆固体废物(25g湿/L)在最大CH4产生率为25 mmol/m~3/d的条件下转化为CH4，30d后固体降解率为15%，表明对于大豆固体废物的处理，采用由水解和产酸反应器组成的两级甲烷发酵工艺和可利用耐卤性产甲烷菌的甲烷形成反应器进行固体还原是可行的。