Immunodiagnosis for echinococcosis: international standardization, early diagnosis and evaluation of prognosis

本研究对肺泡棘球蚴病（AE）患者进行血清学鉴定的主要结果如下。利用重组Em18 （RecEm18）建立一种新的肺泡包虫病的血清诊断方法（Sako et al. 2002）。Journal of Clinical Microbiology, 40, 2769-2765)和亲和纯化的Em18 （Xiao et al.提交发表），并证明Em18是半胱氨酸蛋白酶降解EM10的产物，与人类EMR蛋白的同源性最低，预计在所有其他候选蛋白抗原中具有最高的B细胞表位活性。RecEm18的有效性已经通过法国小组的盲测进行了评估（Ito et al. 2002）。临床微生物学杂志，40(4):461 - 465。法国和瑞士的研究小组刚刚开始使用RecEm18来确认AE。另一种棘球蚴病，囊性棘球蚴病（CE）的血清学分化也很先进（Mamuti et al. 2002）。临床和诊断实验室免疫学9,573 -576)。重组抗原B （RecAgB）的生产已经成功（Mamuti等人在制备中）。利用重组嵌合抗原建立了猪带绦虫神经囊虫病（NCC）的血清学鉴定方法（Sako et al. 2000）。5.中华临床微生物学杂志（英文版），2004,18(4):444 -444。这些鉴定AE、CE和NCC的血清学方法是世界上最好的，已申请国际合作项目，并在多家期刊和书籍中发表。棘球绦虫和带绦虫的分子鉴别工作已经建立。近三年来，我们在三本英文专著中发表了29篇原创论文，14篇论文集和5章。建立快速的血清诊断和分子诊断系统是我们下一步的研究方向。

The main results from this project to identify patients of alveolar echinococcosis (AE) serologically are as followings.1. The establishment of a new serodiagnosis for identification of alveolar echinococcosis using a recombinant Em18 (RecEm18) (Sako et al. 2002. Journal of Clinical Microbiology 40, 2769-2765) and affinity-purified Em18 (Xiao et al. submitted for publication) and demonstration that Em18 is the product of degradation of EM10 by cysteine proteinase and has the least homology with human EMR proteins and be expected to have the highest B cell epitope activity among all other candidate protein antigens.2. The usefulness of RecEm18 has been evaluated as a blind test with French group (Ito et al. 2002. Journal of Clinical Microbiology 40, 4161-4165). Additional work to confirm AE using RecEm18 has just started with French and Swiss groups.3. Serological differentiation of another echinococcosis, cystic echinococcosis (CE) has also been well advanced (Mamuti et al. 2002. Clinical and Diagnostic Laboratory Immunology 9, 573-576). Production of a recombinant Antigen B (RecAgB) has been succeeded (Mamuti et al. in preparation).4. Serology to identify neurocysticercosis (NCC) of Taenia solium has been established using a recombinant chimeric antigen (Sako et al. 2000. Journal of Clinical Microbiology 38, 4439-4444).5. These serological methods to identify AE, CE and NCC are the best in the world and have been applied for international collaboration projects and have been reviewed in several journals and books.6. Molecular work to differentiate Echinococcus species and Taenia species has been established.7. We have published 29 original papers, 14 proceedings and 5 chapters in three books in English last three years. To establish rapid system for these serodiagnosis and molecular diagnosis is the next step of our research.