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Regulation of histamine H1 receptor functions by ribozyme-induced isozyme-specific suppression

通过核酶诱导的同工酶特异性抑制调节组胺 H1 受体功能

基本信息

批准号：
12557231
负责人：
FUKUI Hiroyuki
金额：
$ 8.58万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Histamine H1 receptors mediate type-1 allergy in peripheral tissues and histaminergic neurotransmission in CNS. Activation of the protein kinase C (PKC) induced histamine H1 receptor desensitization, leading to the suppression of the receptor signaling. PKC activation also induced histamine H1 receptor up-regulation through gene expression, enhancing the receptor signaling. Different protein kinase C isozymes were considered to be involved in the two regulatory mechanisms of histamine H1 receptor signaling. In order to identify PKC isozymes in the two mechanisms, specific ribozyme to knock out each PKC isozyme was designed using a vector containing cDNA for ribozyme, pcDNA3. 1 Zeo+CMV(-). Initially, five ribozyme inserts selected from PKC-α sequences at 5'-site, Ribo-PKC-α1 〜 Ribo-PKCα5 were made. Ribozyme generating plasmids, pcDNA3. 1 Zeo+CMV(-)-PKC-α1 〜 pcDNA3. 1 Zeo+CMV(-)-PKC-α5, were constructed using pcDNA3. 1 Zeo+CMV(-). The plasmid was transfected with histamine H1 receptor expressing U373 astrocytoma cells. High transfection rate was essential for the purpose. The transfection rate to U373 cells, however, was not high. Stable transfection was achieved to obtain the PKCα knock out cell line expressing the ribozyme. U373 cells expressing ribozyme, however, failed in knocking out PKCα. Effective transfection reagent with low toxicity, PolyFect Transfection Reagent, showed very high transfection rate (70-80%) to HeLa cells. Transfection of the plasmid and expression of the ribozyme could not knock out PKCα in HeLa cells. Then the plasmid was transfected to HeLa cells with very low level of PKC by the treatment of PKC-activating phorbol ester. The effect of the ribozyme on PKCα generation was very limited in the PMA-treated HeLa cells. In conclusion, establishment of the PKC knock out was not successful due to the difficulties of condition settings and several unknown reasons.

组胺H1受体介导外周组织中的1型变态反应和CNS中的组胺能神经传递。蛋白激酶C（PKC）的激活诱导组胺H1受体脱敏，导致受体信号转导的抑制。PKC激活还通过基因表达诱导组胺H1受体上调，增强受体信号传导。不同的蛋白激酶C同工酶被认为参与了组胺H1受体信号的两种调节机制。为了鉴定这两种机制中的PKC同工酶，使用含有核酶cDNA的载体pcDNA3设计特异性核酶以敲除每种PKC同工酶。1 Zeo + CMV（-）。首先，从PKC-α序列的5 ′-位上选择5个核酶插入片段，即Ribo-PKC-α 1和Ribo-PKC α 5。核酶产生质粒，pcDNA3. 1 Zeo + CMV（-）-PKC-α 1 〜 pcDNA3。1用pcDNA3. 0构建Zeo + CMV（-）-PKC-α 5。1 Zeo + CMV（-）。用表达组胺H1受体的U373星形细胞瘤细胞转染该质粒。高转染率是实现这一目的的关键。但对U373细胞的转染率不高。稳定转染获得表达核酶的PKC α基因敲除细胞株。但表达核酶的U373细胞不能敲除PKC α。高效低毒的转染试剂PolyFect Transfection Reagent对HeLa细胞的转染率高达70 - 80%。该质粒的转染和核酶的表达不能敲除HeLa细胞中的PKC α。然后用PKC激活的佛波酯将该质粒转染到PKC水平很低的HeLa细胞中。在PMA处理的HeLa细胞中，核酶对PKC α产生的影响非常有限。总之，由于条件设置的困难和几个未知原因，PKC敲除的建立并未成功。